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Protein

Tubulin-specific chaperone D

Gene

TBCD

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Tubulin-folding protein implicated in the first step of the tubulin folding pathway and required for tubulin complex assembly. Involved in the regulation of microtubule polymerization or depolymerization, it modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). Its ability to interact with beta tubulin is regulated via its interaction with ARL2. Acts as a GTPase-activating protein (GAP) for ARL2. Induces microtubule disruption in absence of ARL2. Increases degradation of beta tubulin, when overexpressed in polarized cells. Promotes epithelial cell detachment, a process antagonized by ARL2. Induces tight adherens and tight junctions disassembly at the lateral cell membrane. Required for correct assembly and maintenance of the mitotic spindle, and proper progression of mitosis. Involved in neuron morphogenesis.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin-specific chaperone D
Alternative name(s):
Beta-tubulin cofactor D
Tubulin-folding cofactor D
Gene namesi
Name:TBCD
ORF Names:RCJMB04_29e8
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cell junctiontight junction By similarity
  • Lateral cell membrane By similarity
  • Cytoplasm By similarity
  • Cell junctionadherens junction By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Localized in cell-cell contacts.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000800511 – 1019Tubulin-specific chaperone DAdd BLAST1019

Proteomic databases

PaxDbiQ5ZI87.

Interactioni

Subunit structurei

Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PPP2CB (By similarity). Part of a supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Interacts with ARL2; interaction is enhanced with the GDP-bound form of ARL2. Does not interact with ARL3, ARL4A and ARL4D. Interacts with beta tubulin. Interacts with TBCE (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000002281.

Structurei

3D structure databases

ProteinModelPortaliQ5ZI87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati364 – 402HEAT 1Add BLAST39
Repeati856 – 898HEAT 2Add BLAST43

Sequence similaritiesi

Belongs to the TBCD family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1943. Eukaryota.
COG5234. LUCA.
HOGENOMiHOG000231203.
HOVERGENiHBG053297.
InParanoidiQ5ZI87.
PhylomeDBiQ5ZI87.

Family and domain databases

Gene3Di1.25.10.10. 3 hits.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR033162. TBCD.
IPR022577. Tubulin_specific_chaperoneD_C.
PANTHERiPTHR12658:SF3. PTHR12658:SF3. 1 hit.
PfamiView protein in Pfam
PF12612. TFCD_C. 1 hit.
SUPFAMiSSF48371. SSF48371. 3 hits.

Sequencei

Sequence statusi: Complete.

Q5ZI87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGETDGAG SEESGSREAD VISRGNILES FTESQEVRAL LGNLRTVYGD
60 70 80 90 100
PVAQEVIVEK FIVIMDKYQE QPHLLDRHLE WMMNMLLDII RDSGSPPVLF
110 120 130 140 150
HLAFKFLYII TKVRGYKLFL RLFPHEVTDL QPVLDMIVDQ NPKDCETWET
160 170 180 190 200
RYMLLLWLSM ICLIPFDLAR FDGNILSEEG HTRMPTMDRI LEIAKCYLVV
210 220 230 240 250
SDKARDAAAV LVSKFIVRPD VRQKRMADFL DWTLSMLSKS SFQSMEGTVV
260 270 280 290 300
MNGMLQALAQ LFKHGKREDC LPYAATVLEC LDNCKLSESN QMVLRKLGMK
310 320 330 340 350
LVQRLGLTFV KPKVAKWRYQ RGCRSLAANL QAQSSVMQSQ KITVAANEAE
360 370 380 390 400
DDEEYDIPGE IENVVEQLLV GLKDKDTIVR WSAAKGIGRI TGRLPKELAD
410 420 430 440 450
DVVGSLLDCF SFQETDNAWH GGCLALAELG RRGLLLPSRI SDVVPVILKA
460 470 480 490 500
LTYDEKRGSC SVGSNLRDAA CYLSWAFARA YDPSELIPFI NQISSALVIA
510 520 530 540 550
AVFDRDVNCR RAASAAFQEN VGRQGTFPHG IDILTAADYF AVGNRVNCYL
560 570 580 590 600
TISVYIAGFP EYTQPMIDHL VNMKINHWDS VIRELSTKAL HNLTPRAPEY
610 620 630 640 650
MANVVLPRLL PLSVGTDLHT RHGAILACAE ITHALCKLAE ENNRSITYYF
660 670 680 690 700
NGKSLEGLKQ IHQELCSRQL YRGLGGELMR PAVCTLIEKL SLSKMPFKGD
710 720 730 740 750
PIIEGWQWLI NDSLRSLPLA SCAARQHVKE SAVSALSALC NEYYINENGE
760 770 780 790 800
ADPALQGELV TQYISELQST EQMIRCGFSL ALGALPRFLL KGRLQQVLEG
810 820 830 840 850
LRKVTLITPR DVSFAESRRD ALIAIAEICQ TVGVKGEGSQ EEYICKDNVA
860 870 880 890 900
QIYATLLNCV TDYTTDSRGD VGGWVREAAM TSLMKVTLLL VQNEAELINA
910 920 930 940 950
NICKQIMCWL AQQSAEKIDK FRAHAGSVFL TLLHFDSPPV PHIPHREELE
960 970 980 990 1000
RIFPRSEAET LNWNAASEAF PRITQLLALP AYQYYVLLGL SVSVGGLTET
1010
TGVCAPPDNA GPNAGKRLF
Length:1,019
Mass (Da):113,662
Last modified:November 23, 2004 - v1
Checksum:iEF2FBBE80E159BB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720897 mRNA. Translation: CAG32556.1.
RefSeqiNP_001012819.1. NM_001012801.1.
UniGeneiGga.21815.

Genome annotation databases

GeneIDi417334.
KEGGigga:417334.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTBCD_CHICK
AccessioniPrimary (citable) accession number: Q5ZI87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families