Q5ZHX7 (NB5R2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-cytochrome b5 reductase 2
Short name=b5R.2
EC=1.6.2.2

Gene names

Name:	CYB5R2
ORF Names:	RCJMB04_32e2

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	NADH-cytochrome b5 reductase 2		PRO_0000287551
Regions
	Transmembrane	6 – 26	21	Helical; Potential
	Domain	43 – 155	113	FAD-binding FR-type
	Nucleotide binding	135 – 165	31	FAD By similarity
	Nucleotide binding	174 – 209	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5ZHX7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E454143F776A803A	FASTA	304	33,607
	10 20 30 40 50 60 MEAFSGTPVV VAVAAVAATV LLLLLLRGAG RRPGRPVTLR DPQAKYPLPL VGKEEISHDT 70 80 90 100 110 120 KKFRFGLPSP DHVLGLPVGQ HVYLSAKING NLVIRAYTPV SSDETKGYVD LIIKVYYKNV 130 140 150 160 170 180 NPKFPEGGKM SQYLDSMKIG DVIDFRGPNG LLVYKGSGTF MIKPDKKSEA QRKFAKHLGV 190 200 210 220 230 240 IAGGTGITPM LQLIRHITSD PKDSTKCYLL FANQTEKDIL LRAELEDIAK RHPDQVRLWY 250 260 270 280 290 300 TLDRPPQDWK YSSGFVTADM IKTHLPPPGG ETLILMCGPP PMIQFACQPN LDKLGYPKSS TFSY

References

[1]

"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis." Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M. Genome Biol. 6:R6.1-R6.9(2005) [PubMed: 15642098] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: CB. Tissue: Bursa of Fabricius.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ721007 mRNA. Translation: CAG32666.1.
IPI	IPI00588834.
UniGene	Gga.4373.
3D structure databases
HSSP	HSSP built from PDB template 1NDH based on UniProtKB P83686.
ProteinModelPortal	Q5ZHX7.
SMR	Q5ZHX7. Positions 37-304.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	veNOG10667.
HOGENOM	HBG591994.
HOVERGEN	HBG052580.
InParanoid	Q5ZHX7.
OrthoDB	EOG4WDDCG.
PhylomeDB	Q5ZHX7.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
SUPFAM	SSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R2_CHICK

Accession

Primary (citable) accession number: Q5ZHX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	November 23, 2004
Last modified:	September 21, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program