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Protein

WD repeat domain phosphoinositide-interacting protein 2

Gene

WIPI2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Omegasomes are derived from ER and serve as intermediates for genesis of isolation membrane (also called preautophagosome) from ER cisternae membranes.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy

Enzyme and pathway databases

ReactomeiR-GGA-1632852. Macroautophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat domain phosphoinositide-interacting protein 2
Short name:
WIPI-2
Gene namesi
Name:WIPI2
ORF Names:RCJMB04_35d18
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 14

Subcellular locationi

  • Preautophagosomal structure membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 436436WD repeat domain phosphoinositide-interacting protein 2PRO_0000051443Add
BLAST

Proteomic databases

PaxDbiQ5ZHN3.

Expressioni

Gene expression databases

BgeeiENSGALG00000004557.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007240.

Structurei

3D structure databases

ProteinModelPortaliQ5ZHN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati182 – 22241WD 1Sequence analysisAdd
BLAST
Repeati228 – 26740WD 2Sequence analysisAdd
BLAST
Repeati311 – 34939WD 3Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi223 – 2264FRRG motifBy similarity

Domaini

The FRRG motif is required for recruitment to PtdIns3P.

Sequence similaritiesi

Belongs to the WD repeat SVP1 family.Curated
Contains 3 WD repeats.Sequence analysis

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ5ZHN3.
KOiK17908.
OMAiCNPQGLC.
OrthoDBiEOG091G08ZS.
PhylomeDBiQ5ZHN3.
TreeFamiTF314879.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032911. WIPI2.
[Graphical view]
PANTHERiPTHR11227:SF27. PTHR11227:SF27. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.

Sequencei

Sequence statusi: Complete.

Q5ZHN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLAGQSGDA GSGHLLFANF NQDNTSLAVG SKSGYKFFSL SSVDKLEQIY
60 70 80 90 100
ECTDTEDVCI VERLFSSSLV AIVSLKAPRK LKVCHFKKGT EICNYSYSNT
110 120 130 140 150
ILAVKLNRQR LIVCLEESLY IHNIRDMKVL HTIRETPPNP AGLCALSINN
160 170 180 190 200
DNCYLAYPGS ATIGEVQVFD TINLRAANMI PAHDSPLAAL AFDASGTKLA
210 220 230 240 250
TASEKGTVIR VFSIPEGQKL FEFRRGVKRC VSICSLAFSM DGMFLSASSN
260 270 280 290 300
TETVHIFKLE TVKEKPQEEP TTWTGYFGKV LMASTSYLPS QVTEMFNQGR
310 320 330 340 350
AFATVRLPFC GHKNICALAT IQKIPRLLVG AADGYLYMYN LDPQEGGECT
360 370 380 390 400
LMKQHKLDGS MEPANEILES ASHDRPLVAQ TYSAAVTKGT YVPSSPTRHA
410 420 430
YTEDLGAVGG ACLEDETNSL RLDEDSEHPP MILRTD
Length:436
Mass (Da):47,775
Last modified:November 23, 2004 - v1
Checksum:i8848FD63D5C49EE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ721101 mRNA. Translation: CAG32760.1.
RefSeqiNP_001006162.1. NM_001006162.1.
UniGeneiGga.17346.

Genome annotation databases

EnsembliENSGALT00000007252; ENSGALP00000007240; ENSGALG00000004557.
GeneIDi416481.
KEGGigga:416481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ721101 mRNA. Translation: CAG32760.1.
RefSeqiNP_001006162.1. NM_001006162.1.
UniGeneiGga.17346.

3D structure databases

ProteinModelPortaliQ5ZHN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000007240.

Proteomic databases

PaxDbiQ5ZHN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000007252; ENSGALP00000007240; ENSGALG00000004557.
GeneIDi416481.
KEGGigga:416481.

Organism-specific databases

CTDi26100.

Phylogenomic databases

eggNOGiKOG2110. Eukaryota.
ENOG410XP6Y. LUCA.
GeneTreeiENSGT00730000110845.
HOGENOMiHOG000217544.
HOVERGENiHBG056639.
InParanoidiQ5ZHN3.
KOiK17908.
OMAiCNPQGLC.
OrthoDBiEOG091G08ZS.
PhylomeDBiQ5ZHN3.
TreeFamiTF314879.

Enzyme and pathway databases

ReactomeiR-GGA-1632852. Macroautophagy.

Miscellaneous databases

PROiQ5ZHN3.

Gene expression databases

BgeeiENSGALG00000004557.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
IPR032911. WIPI2.
[Graphical view]
PANTHERiPTHR11227:SF27. PTHR11227:SF27. 2 hits.
SMARTiSM00320. WD40. 3 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiWIPI2_CHICK
AccessioniPrimary (citable) accession number: Q5ZHN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.