Q5ZEJ0 (PAS2B_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxyacyl-CoA dehydratase PASTICCINO 2B
Short name=HACD
Short name=PAS2B
EC=4.2.1.-

Alternative name(s):
Protein tyrosine phosphatase-like protein

Gene names

Name:	PAS2B
Ordered Locus Names:	Os01g0150200, LOC_Os01g05694
ORF Names:	P0009G03.5, OsJ_000378, OsJ_00388

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. May be an anti-phosphatase that prevents CDKA-1 dephosphorylation and activation. Involved in the hormonal control of cell division and differentiation. Required for proliferation control of meristematic and non-meristematic cells. Negative regulator of the cell cycle By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the very long-chain fatty acids dehydratase HACD family.
Sequence caution	The sequence EEE53880.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Developmental protein Lyase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	3-hydroxyacyl-CoA dehydratase PASTICCINO 2B		PRO_0000372482
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 51	19	Lumenal Potential
	Transmembrane	52 – 70	19	Helical; Potential
	Topological domain	71 – 76	6	Cytoplasmic Potential
	Transmembrane	77 – 95	19	Helical; Potential
	Topological domain	96 – 100	5	Lumenal Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Topological domain	122 – 141	20	Cytoplasmic Potential
	Transmembrane	142 – 165	24	Helical; Potential
	Topological domain	166 – 184	19	Lumenal Potential
	Transmembrane	185 – 209	25	Helical; Potential
	Topological domain	210 – 221	12	Cytoplasmic Potential
Sites
	Active site	147	1	By similarity
	Active site	154	1	By similarity
Experimental info
	Sequence conflict	31	1	A → T in AK099465. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q5ZEJ0 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 92C56253A92265FC	FASTA	221	25,017
	10 20 30 40 50 60 MTGVGSAVRR LYLSVYNWAV FFGWAQVLYY AVTTLLESGH EAVYAAVERP LQFAQTAAFL 70 80 90 100 110 120 EILHGLVGLV RSPVSATLPQ IGSRLFLTWG ILWSFPETHS HILVTSLVIS WSITEIIRYS 130 140 150 160 170 180 FFGMKETFGF APSWLLWLRY STFMVLYPTG ISSEVGLIYI ALPYMKATEK YCLRMPNKWN 190 200 210 220 FSFDFSYASI LSLAVYVPGS PHMFTYMLAQ RKKALAKAKA A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP002522 Genomic DNA. Translation: BAD61100.1. AP008207 Genomic DNA. Translation: BAF03946.1. CM000138 Genomic DNA. Translation: EEE53880.1. Sequence problems. AK060935 mRNA. Translation: BAG87629.1. AK099465 mRNA. No translation available.
RefSeq	NP_001042032.1. NM_001048567.1.
UniGene	Os.12757.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4324518.
KEGG	osa:4324518.
Organism-specific databases
Gramene	Q5ZEJ0.
Phylogenomic databases
HOGENOM	HBG736553.
PhylomeDB	Q5ZEJ0.
ProtClustDB	PLN02838.
Family and domain databases
InterPro	IPR007482. Tyr_Pase-like_PTPLA. [Graphical view]
KO	K10703.
PANTHER	PTHR11035. PTPLA. 1 hit.
Pfam	PF04387. PTPLA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAS2B_ORYSJ

Accession

Primary (citable) accession number: Q5ZEJ0
Secondary accession number(s): A2ZPA6, B9ESW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program