Q5ZCL8 (CRS2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic

Alternative name(s):
CRS2-like protein
Chloroplastic RNA splicing factor 2

Gene names

Ordered Locus Names:	Os01g0132800, LOC_Os01g04130
ORF Names:	OSJNBa0083M16.12

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for the splicing of group IIB introns in chloroplasts By similarity.
Subunit structure	Part of large ribonucleo-protein complexes that include group IIB introns and either CAF1 or CAF2 By similarity.
Subcellular location	Plastid › chloroplast stroma By similarity.
Sequence similarities	Belongs to the PTH family. CRS2 subfamily.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Ribonucleoprotein
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell ribonucleoprotein complex Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminoacyl-tRNA hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 259	210	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic		PRO_0000280533

Sequences

	Sequence		Length	Mass (Da)
	Q5ZCL8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F990B1ED5206FB01	FASTA	259	28,480
	10 20 30 40 50 60 MSLAVATPAS ARLSPLTTSS PEPCRRRRLL LSAAAPLRRT RLRRRIAVVA SVPDPAARPA 70 80 90 100 110 120 EYTPWLIAGL GNPGSKYHGT RHNVGFEMVD RIARDEGITM NTIQSKSLLG IGSIGEVPVL 130 140 150 160 170 180 LVKPQSYINY SGEAIGPLAA YYQVPLRHIL VMYDEMSLPN GVLRLQRKGG HGRHNGLQNV 190 200 210 220 230 240 MECLDSSREL PRLSIGIGSP PGKMDTRAFL LQKFSSEERL QIDTALEQGV DAVRTLVLKG 250 FSGSIERFNL VQKYKFHSV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003214 Genomic DNA. Translation: BAD52792.1. AP008207 Genomic DNA. Translation: BAF03849.1. AK068422 mRNA. No translation available.
RefSeq	NP_001041935.1. NM_001048470.1.
UniGene	Os.35230.
3D structure databases
HSSP	HSSP built from PDB template 2PTH based on UniProtKB P0A7D1.
ProteinModelPortal	Q5ZCL8.
SMR	Q5ZCL8. Positions 62-252.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ZCL8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4325801.
KEGG	osa:4325801.
Organism-specific databases
Gramene	Q5ZCL8.
Phylogenomic databases
HOGENOM	HBG610927.
PhylomeDB	Q5ZCL8.
ProtClustDB	CLSN2686133.
Family and domain databases
InterPro	IPR001328. Pept_tRNA_hydro. IPR018171. Pept_tRNA_hydro_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.1470. Pept_tRNA_hydro. 1 hit.
KO	K01056.
PANTHER	PTHR17224. Pept_tRNA_hydro. 1 hit.
Pfam	PF01195. Pept_tRNA_hydro. 1 hit. [Graphical view]
SUPFAM	SSF53178. Pept_tRNA_hydro. 1 hit.
TIGRFAMs	TIGR00447. Pth. 1 hit.
PROSITE	PS01195. PEPT_TRNA_HYDROL_1. 1 hit. PS01196. PEPT_TRNA_HYDROL_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRS2_ORYSJ

Accession

Primary (citable) accession number: Q5ZCL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 20, 2007
Last sequence update:	November 23, 2004
Last modified:	November 16, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program