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Protein

Respiratory burst oxidase homolog protein B

Gene

RBOHB

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi242Calcium1
Metal bindingi244Calcium1
Metal bindingi246Calcium1
Metal bindingi248Calcium; via carbonyl oxygen1
Metal bindingi253Calcium1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi242 – 253Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • NAD(P)H oxidase activity Source: InterPro
  • peroxidase activity Source: UniProtKB-KW
  • protein self-association Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-OSA-209968. Thyroxine biosynthesis.
R-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-6798695. Neutrophil degranulation.

Protein family/group databases

PeroxiBasei5564. OsRboh01.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein B (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHB
Short name:
OsrbohB
Gene namesi
Name:RBOHB
Ordered Locus Names:Os01g0360200, LOC_Os01g25820
ORF Names:B1164G11.26, OsJ_01746
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 355CytoplasmicSequence analysisAdd BLAST355
Transmembranei356 – 376Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini377 – 440ExtracellularSequence analysisAdd BLAST64
Transmembranei441 – 461Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini462 – 496CytoplasmicSequence analysisAdd BLAST35
Transmembranei497 – 517Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini518 – 539ExtracellularSequence analysisAdd BLAST22
Transmembranei540 – 560Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini561 – 568CytoplasmicSequence analysis8
Transmembranei569 – 586Helical; Name=5Sequence analysisAdd BLAST18
Topological domaini587 – 717ExtracellularSequence analysisAdd BLAST131
Transmembranei718 – 738Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini739 – 905CytoplasmicSequence analysisAdd BLAST167

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253E → A: Impaired calcium binding. 1 Publication1
Mutagenesisi260 – 274Missing : Loss of RAC1-binding. 1 PublicationAdd BLAST15
Mutagenesisi273R → N: Reduced RAC1-binding. 1 Publication1
Mutagenesisi277Y → Q: Reduced RAC1-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034201 – 905Respiratory burst oxidase homolog protein BAdd BLAST905

Proteomic databases

PaxDbiQ5ZAJ0.
PRIDEiQ5ZAJ0.

Expressioni

Gene expression databases

GenevisibleiQ5ZAJ0. OS.

Interactioni

Subunit structurei

Monomer and homodimer, stabilized by swapping the EF-hand motifs. Interacts with GTP-bound RAC1.1 Publication

GO - Molecular functioni

  • protein self-association Source: UniProtKB

Protein-protein interaction databases

STRINGi39947.LOC_Os01g25820.1.

Structurei

Secondary structure

1905
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi139 – 156Combined sources18
Helixi161 – 171Combined sources11
Beta strandi176 – 178Combined sources3
Helixi179 – 181Combined sources3
Helixi182 – 186Combined sources5
Helixi192 – 205Combined sources14
Beta strandi210 – 213Combined sources4
Helixi215 – 226Combined sources12
Helixi230 – 241Combined sources12
Helixi251 – 263Combined sources13
Helixi266 – 285Combined sources20
Beta strandi291 – 293Combined sources3
Helixi295 – 302Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A8RX-ray2.40A/B138-313[»]
ProteinModelPortaliQ5ZAJ0.
SMRiQ5ZAJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5ZAJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini229 – 264EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini273 – 308EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini395 – 551Ferric oxidoreductaseAdd BLAST157
Domaini587 – 715FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST129

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 180EF-hand-like 19
Regioni206 – 217EF-hand-like 2Add BLAST12

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ5ZAJ0.
KOiK13447.
OMAiENARFPK.
OrthoDBiEOG093601IF.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZAJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAGMVA AATDQGNSTR SQDDAATLIP NSGNLGSSNR STKTARFKDD
60 70 80 90 100
DELVEITLDV QRDSVAIQEV RGVDEGGSGH GTGFDGLPLV SPSSKSGKLT
110 120 130 140 150
SKLRQVTNGL KMKSSSRKAP SPQAQQSAKR VRKRLDRTKS SAAVALKGLQ
160 170 180 190 200
FVTAKVGNDG WAAVEKRFNQ LQVDGVLLRS RFGKCIGMDG SDEFAVQMFD
210 220 230 240 250
SLARKRGIVK QVLTKDELKD FYEQLTDQGF DNRLRTFFDM VDKNADGRLT
260 270 280 290 300
AEEVKEIIAL SASANKLSKI KERADEYTAL IMEELDPTNL GYIEMEDLEA
310 320 330 340 350
LLLQSPSEAA ARSTTTHSSK LSKALSMKLA SNKEMSPVRH YWQQFMYFLE
360 370 380 390 400
ENWKRSWVMT LWISICIALF IWKFIQYRNR AVFGIMGYCV TTAKGAAETL
410 420 430 440 450
KFNMALVLLP VCRNTITWIR SKTQVGAVVP FNDNINFHKV IAAGVAVGVA
460 470 480 490 500
LHAGAHLTCD FPRLLHASDA QYELMKPFFG EKRPPNYWWF VKGTEGWTGV
510 520 530 540 550
VMVVLMAIAF TLAQPWFRRN KLKDSNPLKK MTGFNAFWFT HHLFVIVYTL
560 570 580 590 600
LFVHGTCLYL SRKWYKKTTW MYLAVPVVLY VSERILRLFR SHDAVGIQKV
610 620 630 640 650
AVYPGNVLAL YMSKPPGFRY RSGQYIFIKC TAVSPYEWHP FSITSAPGDD
660 670 680 690 700
YLSVHIRTRG DWTSRLRTVF SEACRPPTEG ESGLLRADLS KGITDEKARF
710 720 730 740 750
PKLLVDGPYG APAQDYREYD VLLLIGLGIG ATPLISIVKD VLNHIQGEGS
760 770 780 790 800
VGTTEPESSS KAKKKPFMTK RAYFYWVTRE EGSFEWFRGV MNEVSEKDKD
810 820 830 840 850
GVIELHNHCS SVYQEGDARS ALIVMLQELQ HAKKGVDILS GTSVKTHFAR
860 870 880 890 900
PNWRSVFKKV AVSHENQRVG VFYCGEPVLV PQLRQLSADF THKTNTRFDF

HKENF
Length:905
Mass (Da):101,759
Last modified:November 23, 2004 - v1
Checksum:iCF3187424F7BA16A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003444 Genomic DNA. Translation: BAD53393.1.
AP008207 Genomic DNA. Translation: BAF04934.1.
AP014957 Genomic DNA. Translation: BAS72095.1.
CM000138 Genomic DNA. Translation: EEE54557.1.
AY603975 mRNA. No translation available.
RefSeqiXP_015620905.1. XM_015765419.1.
UniGeneiOs.10600.

Genome annotation databases

EnsemblPlantsiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
GeneIDi4326027.
GrameneiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
KEGGiosa:4326027.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003444 Genomic DNA. Translation: BAD53393.1.
AP008207 Genomic DNA. Translation: BAF04934.1.
AP014957 Genomic DNA. Translation: BAS72095.1.
CM000138 Genomic DNA. Translation: EEE54557.1.
AY603975 mRNA. No translation available.
RefSeqiXP_015620905.1. XM_015765419.1.
UniGeneiOs.10600.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3A8RX-ray2.40A/B138-313[»]
ProteinModelPortaliQ5ZAJ0.
SMRiQ5ZAJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g25820.1.

Protein family/group databases

PeroxiBasei5564. OsRboh01.

Proteomic databases

PaxDbiQ5ZAJ0.
PRIDEiQ5ZAJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
GeneIDi4326027.
GrameneiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
KEGGiosa:4326027.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ5ZAJ0.
KOiK13447.
OMAiENARFPK.
OrthoDBiEOG093601IF.

Enzyme and pathway databases

ReactomeiR-OSA-209968. Thyroxine biosynthesis.
R-OSA-3299685. Detoxification of Reactive Oxygen Species.
R-OSA-6798695. Neutrophil degranulation.

Miscellaneous databases

EvolutionaryTraceiQ5ZAJ0.

Gene expression databases

GenevisibleiQ5ZAJ0. OS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHB_ORYSJ
AccessioniPrimary (citable) accession number: Q5ZAJ0
Secondary accession number(s): A0A0P0V2T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.