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Protein

Respiratory burst oxidase homolog protein B

Gene

RBOHB

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent NADPH oxidase that generates superoxide.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi242 – 2421Calcium
Metal bindingi244 – 2441Calcium
Metal bindingi246 – 2461Calcium
Metal bindingi248 – 2481Calcium; via carbonyl oxygen
Metal bindingi253 – 2531Calcium

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi242 – 25312Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • NAD(P)H oxidase activity Source: InterPro
  • peroxidase activity Source: UniProtKB-KW
  • protein self-association Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Calcium, FAD, Flavoprotein, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-OSA-209968. Thyroxine biosynthesis.
R-OSA-3299685. Detoxification of Reactive Oxygen Species.

Protein family/group databases

PeroxiBasei5564. OsRboh01.

Names & Taxonomyi

Protein namesi
Recommended name:
Respiratory burst oxidase homolog protein B (EC:1.11.1.-, EC:1.6.3.-)
Alternative name(s):
NADPH oxidase RBOHB
Short name:
OsrbohB
Gene namesi
Name:RBOHB
Ordered Locus Names:Os01g0360200, LOC_Os01g25820
ORF Names:B1164G11.26, OsJ_01746
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 355355CytoplasmicSequence analysisAdd
BLAST
Transmembranei356 – 37621Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini377 – 44064ExtracellularSequence analysisAdd
BLAST
Transmembranei441 – 46121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini462 – 49635CytoplasmicSequence analysisAdd
BLAST
Transmembranei497 – 51721Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini518 – 53922ExtracellularSequence analysisAdd
BLAST
Transmembranei540 – 56021Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini561 – 5688CytoplasmicSequence analysis
Transmembranei569 – 58618Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini587 – 717131ExtracellularSequence analysisAdd
BLAST
Transmembranei718 – 73821Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini739 – 905167CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi253 – 2531E → A: Impaired calcium binding. 1 Publication
Mutagenesisi260 – 27415Missing : Loss of RAC1-binding. 1 PublicationAdd
BLAST
Mutagenesisi273 – 2731R → N: Reduced RAC1-binding. 1 Publication
Mutagenesisi277 – 2771Y → Q: Reduced RAC1-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 905905Respiratory burst oxidase homolog protein BPRO_0000403420Add
BLAST

Proteomic databases

PaxDbiQ5ZAJ0.

Expressioni

Gene expression databases

ExpressionAtlasiQ5ZAJ0. baseline and differential.
GenevisibleiQ5ZAJ0. OS.

Interactioni

Subunit structurei

Monomer and homodimer, stabilized by swapping the EF-hand motifs. Interacts with GTP-bound RAC1.1 Publication

GO - Molecular functioni

  • protein self-association Source: UniProtKB

Protein-protein interaction databases

STRINGi39947.LOC_Os01g25820.1.

Structurei

Secondary structure

1
905
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi139 – 15618Combined sources
Helixi161 – 17111Combined sources
Beta strandi176 – 1783Combined sources
Helixi179 – 1813Combined sources
Helixi182 – 1865Combined sources
Helixi192 – 20514Combined sources
Beta strandi210 – 2134Combined sources
Helixi215 – 22612Combined sources
Helixi230 – 24112Combined sources
Helixi251 – 26313Combined sources
Helixi266 – 28520Combined sources
Beta strandi291 – 2933Combined sources
Helixi295 – 3028Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A8RX-ray2.40A/B138-313[»]
ProteinModelPortaliQ5ZAJ0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5ZAJ0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini229 – 26436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini273 – 30836EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini395 – 551157Ferric oxidoreductaseAdd
BLAST
Domaini587 – 715129FAD-binding FR-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 1809EF-hand-like 1
Regioni206 – 21712EF-hand-like 2Add
BLAST

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ5ZAJ0.
OMAiENARFPK.
OrthoDBiEOG093601IF.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5ZAJ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADLEAGMVA AATDQGNSTR SQDDAATLIP NSGNLGSSNR STKTARFKDD
60 70 80 90 100
DELVEITLDV QRDSVAIQEV RGVDEGGSGH GTGFDGLPLV SPSSKSGKLT
110 120 130 140 150
SKLRQVTNGL KMKSSSRKAP SPQAQQSAKR VRKRLDRTKS SAAVALKGLQ
160 170 180 190 200
FVTAKVGNDG WAAVEKRFNQ LQVDGVLLRS RFGKCIGMDG SDEFAVQMFD
210 220 230 240 250
SLARKRGIVK QVLTKDELKD FYEQLTDQGF DNRLRTFFDM VDKNADGRLT
260 270 280 290 300
AEEVKEIIAL SASANKLSKI KERADEYTAL IMEELDPTNL GYIEMEDLEA
310 320 330 340 350
LLLQSPSEAA ARSTTTHSSK LSKALSMKLA SNKEMSPVRH YWQQFMYFLE
360 370 380 390 400
ENWKRSWVMT LWISICIALF IWKFIQYRNR AVFGIMGYCV TTAKGAAETL
410 420 430 440 450
KFNMALVLLP VCRNTITWIR SKTQVGAVVP FNDNINFHKV IAAGVAVGVA
460 470 480 490 500
LHAGAHLTCD FPRLLHASDA QYELMKPFFG EKRPPNYWWF VKGTEGWTGV
510 520 530 540 550
VMVVLMAIAF TLAQPWFRRN KLKDSNPLKK MTGFNAFWFT HHLFVIVYTL
560 570 580 590 600
LFVHGTCLYL SRKWYKKTTW MYLAVPVVLY VSERILRLFR SHDAVGIQKV
610 620 630 640 650
AVYPGNVLAL YMSKPPGFRY RSGQYIFIKC TAVSPYEWHP FSITSAPGDD
660 670 680 690 700
YLSVHIRTRG DWTSRLRTVF SEACRPPTEG ESGLLRADLS KGITDEKARF
710 720 730 740 750
PKLLVDGPYG APAQDYREYD VLLLIGLGIG ATPLISIVKD VLNHIQGEGS
760 770 780 790 800
VGTTEPESSS KAKKKPFMTK RAYFYWVTRE EGSFEWFRGV MNEVSEKDKD
810 820 830 840 850
GVIELHNHCS SVYQEGDARS ALIVMLQELQ HAKKGVDILS GTSVKTHFAR
860 870 880 890 900
PNWRSVFKKV AVSHENQRVG VFYCGEPVLV PQLRQLSADF THKTNTRFDF

HKENF
Length:905
Mass (Da):101,759
Last modified:November 23, 2004 - v1
Checksum:iCF3187424F7BA16A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003444 Genomic DNA. Translation: BAD53393.1.
AP008207 Genomic DNA. Translation: BAF04934.1.
AP014957 Genomic DNA. Translation: BAS72095.1.
CM000138 Genomic DNA. Translation: EEE54557.1.
AY603975 mRNA. No translation available.
RefSeqiXP_015620905.1. XM_015765419.1.
UniGeneiOs.10600.

Genome annotation databases

EnsemblPlantsiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
GeneIDi4326027.
GrameneiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003444 Genomic DNA. Translation: BAD53393.1.
AP008207 Genomic DNA. Translation: BAF04934.1.
AP014957 Genomic DNA. Translation: BAS72095.1.
CM000138 Genomic DNA. Translation: EEE54557.1.
AY603975 mRNA. No translation available.
RefSeqiXP_015620905.1. XM_015765419.1.
UniGeneiOs.10600.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3A8RX-ray2.40A/B138-313[»]
ProteinModelPortaliQ5ZAJ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os01g25820.1.

Protein family/group databases

PeroxiBasei5564. OsRboh01.

Proteomic databases

PaxDbiQ5ZAJ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.
GeneIDi4326027.
GrameneiOS01T0360200-01; OS01T0360200-01; OS01G0360200.
OS01T0360200-03; OS01T0360200-03; OS01G0360200.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000216670.
InParanoidiQ5ZAJ0.
OMAiENARFPK.
OrthoDBiEOG093601IF.

Enzyme and pathway databases

ReactomeiR-OSA-209968. Thyroxine biosynthesis.
R-OSA-3299685. Detoxification of Reactive Oxygen Species.

Miscellaneous databases

EvolutionaryTraceiQ5ZAJ0.

Gene expression databases

ExpressionAtlasiQ5ZAJ0. baseline and differential.
GenevisibleiQ5ZAJ0. OS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR013623. NADPH_Ox.
IPR029654. RBOHB.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERiPTHR11972:SF57. PTHR11972:SF57. 1 hit.
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PF08414. NADPH_Ox. 1 hit.
[Graphical view]
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRBOHB_ORYSJ
AccessioniPrimary (citable) accession number: Q5ZAJ0
Secondary accession number(s): A0A0P0V2T2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.