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Protein

Endoglucanase 18

Gene

Os06g0715300

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei436PROSITE-ProRule annotation1
Active sitei482PROSITE-ProRule annotation1
Active sitei491PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH9. Glycoside Hydrolase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 18 (EC:3.2.1.4)
Alternative name(s):
Endo-1,4-beta glucanase 18
Gene namesi
Ordered Locus Names:Os06g0715300, LOC_Os06g50140
ORF Names:P0481E08.13
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 35CytoplasmicSequence analysisAdd BLAST35
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Topological domaini57 – 518ExtracellularSequence analysisAdd BLAST462

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002492951 – 518Endoglucanase 18Add BLAST518

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi214N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi251N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi272N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi477N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5Z9P8.

Expressioni

Gene expression databases

GenevisibleiQ5Z9P8. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g50140.1.

Structurei

3D structure databases

ProteinModelPortaliQ5Z9P8.
SMRiQ5Z9P8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IDXW. Eukaryota.
ENOG410XPC9. LUCA.
HOGENOMiHOG000021033.
InParanoidiQ5Z9P8.
OMAiGHKWLET.
OrthoDBiEOG093609RT.

Family and domain databases

InterProiView protein in InterPro
IPR008928. 6-hairpin_glycosidase-like.
IPR001701. Glyco_hydro_9.
IPR018221. Glyco_hydro_9_His_AS.
PfamiView protein in Pfam
PF00759. Glyco_hydro_9. 1 hit.
SUPFAMiSSF48208. SSF48208. 1 hit.
PROSITEiView protein in PROSITE
PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5Z9P8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANCVRCCCW LLVLMLMALA ITAAVVFVRY KNGEGVFPFP GVPGAVDHKY
60 70 80 90 100
ADALAVALQF FQVQKSGKLV NNTIHWRGDS ALDDGKEAGI DLSKGMYDAG
110 120 130 140 150
DHMKFGFPMA FTATMLSWSV LEYGDAMRAA DQRDSAIDAL NWIMDYLVNA
160 170 180 190 200
HPSDDVLYIQ VGDPKADHKC WERPEKMKEK RPLTKITPKS PGSDVAAETA
210 220 230 240 250
AAMAAASLVY KTINKTYSSS LLDHGERLFA FADKHRGSYT RTFPELSAFY
260 270 280 290 300
NSTTYQDELL WAASWLYHAT GNHSYLAYAT GKNKDFADLG NPRYFSWDDK
310 320 330 340 350
RAGTEVLLSR VSFFASQGSD VAQDDVLGMY KQTADAVMCI LLPDSETAAF
360 370 380 390 400
RTEGGLLYVA EWNSLQHPVA SAFLAAVYSD YMQSSGKTEL SCSGQGFSPA
410 420 430 440 450
DLRKFAKSQA DYLLGSNPMK ISYLVGYGDR YPEKVHHRGA SIPEDVDTGC
460 470 480 490 500
DGHKWLETSK PNPNVATGAL VGGPYKNDSF VDERDNVMQN EATTYNSALV
510
AGLLSALVST SSLARSLS
Length:518
Mass (Da):56,741
Last modified:November 23, 2004 - v1
Checksum:iF6DA8EC0D7951B8C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti450C → Y in AK121369 (PubMed:12869764).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003614 Genomic DNA. Translation: BAD53575.1.
AP008212 Genomic DNA. Translation: BAF20497.1.
AP014962 Genomic DNA. Translation: BAS99495.1.
AK121369 mRNA. No translation available.
RefSeqiXP_015642677.1. XM_015787191.1.
UniGeneiOs.50481.

Genome annotation databases

EnsemblPlantsiOS06T0715300-01; OS06T0715300-01; OS06G0715300.
GeneIDi4342070.
GrameneiOS06T0715300-01; OS06T0715300-01; OS06G0715300.
KEGGiosa:4342070.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGUN18_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z9P8
Secondary accession number(s): A0A0P0X0U4, Q0D9H6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families