Q5Z9P8 (GUN18_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 18
EC=3.2.1.4

Alternative name(s):
Endo-1,4-beta glucanase 18

Gene names

Ordered Locus Names:	Os06g0715300, LOC_Os06g50140
ORF Names:	P0481E08.13

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Endoglucanase 18		PRO_0000249295
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Potential
	Topological domain	57 – 518	462	Extracellular Potential
Sites
	Active site	436	1	By similarity
	Active site	482	1	By similarity
	Active site	491	1	By similarity
Amino acid modifications
	Glycosylation	71	1	N-linked (GlcNAc...) Potential
	Glycosylation	214	1	N-linked (GlcNAc...) Potential
	Glycosylation	251	1	N-linked (GlcNAc...) Potential
	Glycosylation	272	1	N-linked (GlcNAc...) Potential
	Glycosylation	477	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	450	1	C → Y in AK121369. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5Z9P8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F6DA8EC0D7951B8C	FASTA	518	56,741
	10 20 30 40 50 60 MANCVRCCCW LLVLMLMALA ITAAVVFVRY KNGEGVFPFP GVPGAVDHKY ADALAVALQF 70 80 90 100 110 120 FQVQKSGKLV NNTIHWRGDS ALDDGKEAGI DLSKGMYDAG DHMKFGFPMA FTATMLSWSV 130 140 150 160 170 180 LEYGDAMRAA DQRDSAIDAL NWIMDYLVNA HPSDDVLYIQ VGDPKADHKC WERPEKMKEK 190 200 210 220 230 240 RPLTKITPKS PGSDVAAETA AAMAAASLVY KTINKTYSSS LLDHGERLFA FADKHRGSYT 250 260 270 280 290 300 RTFPELSAFY NSTTYQDELL WAASWLYHAT GNHSYLAYAT GKNKDFADLG NPRYFSWDDK 310 320 330 340 350 360 RAGTEVLLSR VSFFASQGSD VAQDDVLGMY KQTADAVMCI LLPDSETAAF RTEGGLLYVA 370 380 390 400 410 420 EWNSLQHPVA SAFLAAVYSD YMQSSGKTEL SCSGQGFSPA DLRKFAKSQA DYLLGSNPMK 430 440 450 460 470 480 ISYLVGYGDR YPEKVHHRGA SIPEDVDTGC DGHKWLETSK PNPNVATGAL VGGPYKNDSF 490 500 510 VDERDNVMQN EATTYNSALV AGLLSALVST SSLARSLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003614 Genomic DNA. Translation: BAD53575.1. AP008212 Genomic DNA. Translation: BAF20497.1. AK121369 mRNA. No translation available.
RefSeq	NP_001058583.1. NM_001065118.1.
UniGene	Os.50481.
3D structure databases
HSSP	HSSP built from PDB template 1TF4 based on UniProtKB P26221.
ProteinModelPortal	Q5Z9P8.
ModBase	Search...
Protein family/group databases
CAZy	GH9. Glycoside Hydrolase Family 9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g50140.1; LOC_Os06g50140.1; LOC_Os06g50140.
GeneID	4342070.
KEGG	osa:4342070.
Organism-specific databases
Gramene	Q5Z9P8.
Phylogenomic databases
eggNOG	NOG05134.
HOGENOM	HOG000021033.
OMA	TTYQDEL.
ProtClustDB	PLN02345.
Gene expression databases
ArrayExpress	Q5Z9P8.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Pfam	PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN18_ORYSJ

Accession

Primary (citable) accession number: Q5Z9P8
Secondary accession number(s): Q0D9H6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program