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Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in defense signaling pathway. Phosphorylates EREBP1 transcriptional activator in vitro. Enhances DNA-binding activity of EREBP1 to the GCC box element of pathogenesis-related (PR) gene promoters.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to hydrogen peroxide, salicylic acid, jasmonic acid, ethylene, fungal elicitor and infection with rice blast fungus (M.grisea).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116ATPPROSITE-ProRule annotation1
Active sitei213Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi93 – 101ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-4086398 Ca2+ pathway
R-OSA-5687128 MAPK6/MAPK4 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
MAP kinase 12
Alternative name(s):
Blast- and wound-induced MAP kinase 1
MAP kinase 1
OsBWMK1
OsMAPK1
Gene namesi
Name:MPK12
Synonyms:BWMK1, MAPK1
Ordered Locus Names:Os06g0708000, LOC_Os06g49430
ORF Names:P0621D05.40-1, P0621D05.40-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397551 – 580Mitogen-activated protein kinase 12Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei249Phosphothreonine1 Publication1
Modified residuei251Phosphotyrosine1 Publication1

Post-translational modificationi

Dually phosphorylated on Thr-249 and Tyr-251, which activates the enzyme (By similarity). Phosphorylated on tyrosine residue.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5Z9J0

PTM databases

iPTMnetiQ5Z9J0

Expressioni

Inductioni

By hydrogen peroxide, salicylic acid (SA), jasmonic acid (JA), ethylene, abscisic acid (ABA), fungal elicitor, infection with rice blast fungus (M.grisea) and wounding.3 Publications

Gene expression databases

ExpressionAtlasiQ5Z9J0 differential
GenevisibleiQ5Z9J0 OS

Interactioni

Subunit structurei

Interacts with EREBP1.1 Publication

Protein-protein interaction databases

BioGridi811054, 2 interactors
STRINGi39947.LOC_Os06g49430.1

Structurei

3D structure databases

ProteinModelPortaliQ5Z9J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 378Protein kinasePROSITE-ProRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni325 – 506Required for kinase activity and nuclear localizationAdd BLAST182

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi249 – 251TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0660 Eukaryota
ENOG410XNY0 LUCA
HOGENOMiHOG000233024
InParanoidiQ5Z9J0
OMAiCEATRIL
OrthoDBiEOG093605CF

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5Z9J0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGGGTLVDG FRRLFHRRTA SGSNQSSNAG EEAASSDLEV ADDPDLVALR
60 70 80 90 100
SIRIRVPKRK MPLPVESHKK NTVEMEFFTE YGEASQYQIQ EVIGKGSYGV
110 120 130 140 150
VAAAVDTRTG ERVAIKKIND VFEHVSDATR ILREIKLLRL LRHPDIVEIK
160 170 180 190 200
HIMLPPSRRE FQDIYVVFEL MESDLHQVIR ANDDLTPEHY QFFLYQLLRA
210 220 230 240 250
LKYIHAANVF HRDLKPKNIL ANSDCKLKIC DFGLARASFN DAPSAIFWTD
260 270 280 290 300
YVATRWYRAP ELCGSFFSKY TPAIDIWSIG CIFAELLTGR PLFPGKNVVH
310 320 330 340 350
QLDIITDLLG TPSSETLSRI RNEKARRYLS TMRKKHAVPF SQKFRNTDPL
360 370 380 390 400
ALRLLERLLA FDPKDRSSAE EALADPYFAS LANVEREPSR HPISKLEFEF
410 420 430 440 450
ERRKLTKDDV RELIYREILE YHPQMLQEYM KGGEQISFLY PSGVDRFKRQ
460 470 480 490 500
FAHLEENYSK GERGSPLQRK HASLPRERVG VSKDGYNQQN TNDQERSADS
510 520 530 540 550
VARTTVSPPM SQDAQQHGSA GQNGVTSTDL SSRSYLKSAS ISASKCVAVK
560 570 580
DNKEPEDDYI SEEMEGSVDG LSEQVSRMHS
Length:580
Mass (Da):65,945
Last modified:June 13, 2006 - v2
Checksum:i793AA85445857EF6
GO
Isoform 2 (identifier: Q5Z9J0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:506
Mass (Da):57,946
Checksum:iB05509010B2673C4
GO

Sequence cautioni

The sequence AAF23902 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti196Q → R in AK066531 (PubMed:12869764).Curated1
Sequence conflicti367S → P in AAD52659 (PubMed:10624015).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0192641 – 74Missing in isoform 2. 1 PublicationAdd BLAST74

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177392 mRNA Translation: AAD52659.1
AP003621 Genomic DNA Translation: BAD53616.1
AP003621 Genomic DNA Translation: BAD53617.1
AP008212 Genomic DNA Translation: BAF20444.1
AP014962 Genomic DNA Translation: BAS99425.1
AK066531 mRNA No translation available.
AK067925 mRNA No translation available.
AF194415 mRNA Translation: AAF23902.1 Different initiation.
RefSeqiXP_015643068.1, XM_015787582.1 [Q5Z9J0-1]
UniGeneiOs.7913

Genome annotation databases

EnsemblPlantsiOs06t0708000-01; Os06t0708000-01; Os06g0708000 [Q5Z9J0-1]
Os06t0708000-02; Os06t0708000-02; Os06g0708000 [Q5Z9J0-2]
Os06t0708000-03; Os06t0708000-03; Os06g0708000 [Q5Z9J0-2]
GeneIDi4342017
GrameneiOs06t0708000-01; Os06t0708000-01; Os06g0708000 [Q5Z9J0-1]
Os06t0708000-02; Os06t0708000-02; Os06g0708000 [Q5Z9J0-2]
Os06t0708000-03; Os06t0708000-03; Os06g0708000 [Q5Z9J0-2]
KEGGiosa:4342017

Keywords - Coding sequence diversityi

Alternative splicing

Entry informationi

Entry nameiMPK12_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z9J0
Secondary accession number(s): Q0D9M9
, Q5Z9J1, Q9SE23, Q9SPF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: May 23, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

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