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Q5Z9J0

- MPK12_ORYSJ

UniProt

Q5Z9J0 - MPK12_ORYSJ

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Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in defense signaling pathway. Phosphorylates EREBP1 transcriptional activator in vitro. Enhances DNA-binding activity of EREBP1 to the GCC box element of pathogenesis-related (PR) gene promoters.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation (By similarity). Activated in response to hydrogen peroxide, salicylic acid, jasmonic acid, ethylene, fungal elicitor and infection with rice blast fungus (M.grisea).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161ATPPROSITE-ProRule annotation
Active sitei213 – 2131Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi93 – 1019ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 12 (EC:2.7.11.24)
Short name:
MAP kinase 12
Alternative name(s):
Blast- and wound-induced MAP kinase 1
MAP kinase 1
OsBWMK1
OsMAPK1
Gene namesi
Name:MPK12
Synonyms:BWMK1, MAPK1
Ordered Locus Names:Os06g0708000, LOC_Os06g49430
ORF Names:P0621D05.40-1, P0621D05.40-2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ5Z9J0.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication
Note: Translocated into the nucleus in response to phosphorylation.Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 580580Mitogen-activated protein kinase 12PRO_0000239755Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei249 – 2491PhosphothreonineBy similarity
Modified residuei251 – 2511Phosphotyrosine1 Publication

Post-translational modificationi

Dually phosphorylated on Thr-249 and Tyr-251, which activates the enzyme (By similarity). Phosphorylated on tyrosine residue.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5Z9J0.

Expressioni

Inductioni

By hydrogen peroxide, salicylic acid (SA), jasmonic acid (JA), ethylene, abscisic acid (ABA), fungal elicitor, infection with rice blast fungus (M.grisea) and wounding.3 Publications

Gene expression databases

ExpressionAtlasiQ5Z9J0. baseline.

Interactioni

Subunit structurei

Interacts with EREBP1.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5Z9J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 378292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni325 – 506182Required for kinase activity and nuclear localizationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi249 – 2513TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5Z9J0.
OMAiFTHKFRN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5Z9J0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGGGGTLVDG FRRLFHRRTA SGSNQSSNAG EEAASSDLEV ADDPDLVALR
60 70 80 90 100
SIRIRVPKRK MPLPVESHKK NTVEMEFFTE YGEASQYQIQ EVIGKGSYGV
110 120 130 140 150
VAAAVDTRTG ERVAIKKIND VFEHVSDATR ILREIKLLRL LRHPDIVEIK
160 170 180 190 200
HIMLPPSRRE FQDIYVVFEL MESDLHQVIR ANDDLTPEHY QFFLYQLLRA
210 220 230 240 250
LKYIHAANVF HRDLKPKNIL ANSDCKLKIC DFGLARASFN DAPSAIFWTD
260 270 280 290 300
YVATRWYRAP ELCGSFFSKY TPAIDIWSIG CIFAELLTGR PLFPGKNVVH
310 320 330 340 350
QLDIITDLLG TPSSETLSRI RNEKARRYLS TMRKKHAVPF SQKFRNTDPL
360 370 380 390 400
ALRLLERLLA FDPKDRSSAE EALADPYFAS LANVEREPSR HPISKLEFEF
410 420 430 440 450
ERRKLTKDDV RELIYREILE YHPQMLQEYM KGGEQISFLY PSGVDRFKRQ
460 470 480 490 500
FAHLEENYSK GERGSPLQRK HASLPRERVG VSKDGYNQQN TNDQERSADS
510 520 530 540 550
VARTTVSPPM SQDAQQHGSA GQNGVTSTDL SSRSYLKSAS ISASKCVAVK
560 570 580
DNKEPEDDYI SEEMEGSVDG LSEQVSRMHS
Length:580
Mass (Da):65,945
Last modified:June 13, 2006 - v2
Checksum:i793AA85445857EF6
GO
Isoform 2 (identifier: Q5Z9J0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.

Show »
Length:506
Mass (Da):57,946
Checksum:iB05509010B2673C4
GO

Sequence cautioni

The sequence AAF23902.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti196 – 1961Q → R in AK066531. (PubMed:12869764)Curated
Sequence conflicti367 – 3671S → P in AAD52659. (PubMed:10624015)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7474Missing in isoform 2. 1 PublicationVSP_019264Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177392 mRNA. Translation: AAD52659.1.
AP003621 Genomic DNA. Translation: BAD53616.1.
AP003621 Genomic DNA. Translation: BAD53617.1.
AK066531 mRNA. No translation available.
AK067925 mRNA. No translation available.
AF194415 mRNA. Translation: AAF23902.1. Different initiation.
RefSeqiNP_001058530.1. NM_001065065.1. [Q5Z9J0-1]
UniGeneiOs.7913.

Genome annotation databases

EnsemblPlantsiOS06T0708000-01; OS06T0708000-01; OS06G0708000. [Q5Z9J0-1]
GeneIDi4342017.
KEGGiosa:4342017.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF177392 mRNA. Translation: AAD52659.1 .
AP003621 Genomic DNA. Translation: BAD53616.1 .
AP003621 Genomic DNA. Translation: BAD53617.1 .
AK066531 mRNA. No translation available.
AK067925 mRNA. No translation available.
AF194415 mRNA. Translation: AAF23902.1 . Different initiation.
RefSeqi NP_001058530.1. NM_001065065.1. [Q5Z9J0-1 ]
UniGenei Os.7913.

3D structure databases

ProteinModelPortali Q5Z9J0.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5Z9J0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0708000-01 ; OS06T0708000-01 ; OS06G0708000 . [Q5Z9J0-1 ]
GeneIDi 4342017.
KEGGi osa:4342017.

Organism-specific databases

Gramenei Q5Z9J0.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q5Z9J0.
OMAi FTHKFRN.

Gene expression databases

ExpressionAtlasi Q5Z9J0. baseline.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice."
    He C., Fong S.H.T., Yang D., Wang G.-L.
    Mol. Plant Microbe Interact. 12:1064-1073(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME REGULATION, INDUCTION, PHOSPHORYLATION.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: cv. Nipponbare.
  4. "BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor."
    Cheong Y.H., Moon B.C., Kim J.K., Kim C.Y., Kim M.C., Kim I.H., Park C.Y., Kim J.C., Park B.O., Koo S.C., Yoon H.W., Chung W.S., Lim C.O., Lee S.Y., Cho M.J.
    Plant Physiol. 132:1961-1972(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-580 (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH EREBP1.
  5. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK12_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z9J0
Secondary accession number(s): Q5Z9J1, Q9SE23, Q9SPF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: October 29, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3