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Q5Z9J0 (MPK12_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 12

Short name=MAP kinase 12
EC=2.7.11.24
Alternative name(s):
Blast- and wound-induced MAP kinase 1
MAP kinase 1
OsBWMK1
OsMAPK1
Gene names
Name:MPK12
Synonyms:BWMK1, MAPK1
Ordered Locus Names:Os06g0708000, LOC_Os06g49430
ORF Names:P0621D05.40-1, P0621D05.40-2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length580 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be involved in defense signaling pathway. Phosphorylates EREBP1 transcriptional activator in vitro. Enhances DNA-binding activity of EREBP1 to the GCC box element of pathogenesis-related (PR) gene promoters. Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to hydrogen peroxide, salicylic acid, jasmonic acid, ethylene, fungal elicitor and infection with rice blast fungus (M.grisea). Ref.1 Ref.4

Subunit structure

Interacts with EREBP1. Ref.4

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation Probable. Ref.4

Induction

By hydrogen peroxide, salicylic acid (SA), jasmonic acid (JA), ethylene, abscisic acid (ABA), fungal elicitor, infection with rice blast fungus (M.grisea) and wounding. Ref.1 Ref.4 Ref.5

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-249 and Tyr-251, which activates the enzyme By similarity. Phosphorylated on tyrosine residue. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAF23902.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5Z9J0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5Z9J0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 580580Mitogen-activated protein kinase 12
PRO_0000239755

Regions

Domain87 – 378292Protein kinase
Nucleotide binding93 – 1019ATP By similarity
Region325 – 506182Required for kinase activity and nuclear localization
Motif249 – 2513TXY

Sites

Active site2131Proton acceptor By similarity
Binding site1161ATP By similarity

Amino acid modifications

Modified residue2491Phosphothreonine By similarity
Modified residue2511Phosphotyrosine Probable

Natural variations

Alternative sequence1 – 7474Missing in isoform 2.
VSP_019264

Experimental info

Sequence conflict1961Q → R in AK066531. Ref.3
Sequence conflict3671S → P in AAD52659. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: 793AA85445857EF6

FASTA58065,945
        10         20         30         40         50         60 
MGGGGTLVDG FRRLFHRRTA SGSNQSSNAG EEAASSDLEV ADDPDLVALR SIRIRVPKRK 

        70         80         90        100        110        120 
MPLPVESHKK NTVEMEFFTE YGEASQYQIQ EVIGKGSYGV VAAAVDTRTG ERVAIKKIND 

       130        140        150        160        170        180 
VFEHVSDATR ILREIKLLRL LRHPDIVEIK HIMLPPSRRE FQDIYVVFEL MESDLHQVIR 

       190        200        210        220        230        240 
ANDDLTPEHY QFFLYQLLRA LKYIHAANVF HRDLKPKNIL ANSDCKLKIC DFGLARASFN 

       250        260        270        280        290        300 
DAPSAIFWTD YVATRWYRAP ELCGSFFSKY TPAIDIWSIG CIFAELLTGR PLFPGKNVVH 

       310        320        330        340        350        360 
QLDIITDLLG TPSSETLSRI RNEKARRYLS TMRKKHAVPF SQKFRNTDPL ALRLLERLLA 

       370        380        390        400        410        420 
FDPKDRSSAE EALADPYFAS LANVEREPSR HPISKLEFEF ERRKLTKDDV RELIYREILE 

       430        440        450        460        470        480 
YHPQMLQEYM KGGEQISFLY PSGVDRFKRQ FAHLEENYSK GERGSPLQRK HASLPRERVG 

       490        500        510        520        530        540 
VSKDGYNQQN TNDQERSADS VARTTVSPPM SQDAQQHGSA GQNGVTSTDL SSRSYLKSAS 

       550        560        570        580 
ISASKCVAVK DNKEPEDDYI SEEMEGSVDG LSEQVSRMHS 

« Hide

Isoform 2 [UniParc].

Checksum: B05509010B2673C4
Show »

FASTA50657,946

References

« Hide 'large scale' references
[1]"BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice."
He C., Fong S.H.T., Yang D., Wang G.-L.
Mol. Plant Microbe Interact. 12:1064-1073(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME REGULATION, INDUCTION, PHOSPHORYLATION.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Nipponbare.
[4]"BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor."
Cheong Y.H., Moon B.C., Kim J.K., Kim C.Y., Kim M.C., Kim I.H., Park C.Y., Kim J.C., Park B.O., Koo S.C., Yoon H.W., Chung W.S., Lim C.O., Lee S.Y., Cho M.J.
Plant Physiol. 132:1961-1972(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-580 (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH EREBP1.
[5]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF177392 mRNA. Translation: AAD52659.1.
AP003621 Genomic DNA. Translation: BAD53616.1.
AP003621 Genomic DNA. Translation: BAD53617.1.
AK066531 mRNA. No translation available.
AK067925 mRNA. No translation available.
AF194415 mRNA. Translation: AAF23902.1. Different initiation.
RefSeqNP_001058530.1. NM_001065065.1. [Q5Z9J0-1]
UniGeneOs.7913.

3D structure databases

ProteinModelPortalQ5Z9J0.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ5Z9J0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0708000-01; OS06T0708000-01; OS06G0708000. [Q5Z9J0-1]
GeneID4342017.
KEGGosa:4342017.

Organism-specific databases

GrameneQ5Z9J0.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
OMAFTHKFRN.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK12_ORYSJ
AccessionPrimary (citable) accession number: Q5Z9J0
Secondary accession number(s): Q5Z9J1, Q9SE23, Q9SPF0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: May 14, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations