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Q5Z9J0

- MPK12_ORYSJ

UniProt

Q5Z9J0 - MPK12_ORYSJ

Protein

Mitogen-activated protein kinase 12

Gene

MPK12

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    May be involved in defense signaling pathway. Phosphorylates EREBP1 transcriptional activator in vitro. Enhances DNA-binding activity of EREBP1 to the GCC box element of pathogenesis-related (PR) gene promoters.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation By similarity. Activated in response to hydrogen peroxide, salicylic acid, jasmonic acid, ethylene, fungal elicitor and infection with rice blast fungus (M.grisea).By similarity2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161ATPPROSITE-ProRule annotation
    Active sitei213 – 2131Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi93 – 1019ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Plant defense

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 12 (EC:2.7.11.24)
    Short name:
    MAP kinase 12
    Alternative name(s):
    Blast- and wound-induced MAP kinase 1
    MAP kinase 1
    OsBWMK1
    OsMAPK1
    Gene namesi
    Name:MPK12
    Synonyms:BWMK1, MAPK1
    Ordered Locus Names:Os06g0708000, LOC_Os06g49430
    ORF Names:P0621D05.40-1, P0621D05.40-2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ5Z9J0.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Translocated into the nucleus in response to phosphorylation.Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 580580Mitogen-activated protein kinase 12PRO_0000239755Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei249 – 2491PhosphothreonineBy similarity
    Modified residuei251 – 2511Phosphotyrosine1 Publication

    Post-translational modificationi

    Dually phosphorylated on Thr-249 and Tyr-251, which activates the enzyme By similarity. Phosphorylated on tyrosine residue.By similarity1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ5Z9J0.

    Expressioni

    Inductioni

    By hydrogen peroxide, salicylic acid (SA), jasmonic acid (JA), ethylene, abscisic acid (ABA), fungal elicitor, infection with rice blast fungus (M.grisea) and wounding.3 Publications

    Interactioni

    Subunit structurei

    Interacts with EREBP1.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ5Z9J0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini87 – 378292Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni325 – 506182Required for kinase activity and nuclear localizationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi249 – 2513TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    OMAiFTHKFRN.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5Z9J0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGGGGTLVDG FRRLFHRRTA SGSNQSSNAG EEAASSDLEV ADDPDLVALR    50
    SIRIRVPKRK MPLPVESHKK NTVEMEFFTE YGEASQYQIQ EVIGKGSYGV 100
    VAAAVDTRTG ERVAIKKIND VFEHVSDATR ILREIKLLRL LRHPDIVEIK 150
    HIMLPPSRRE FQDIYVVFEL MESDLHQVIR ANDDLTPEHY QFFLYQLLRA 200
    LKYIHAANVF HRDLKPKNIL ANSDCKLKIC DFGLARASFN DAPSAIFWTD 250
    YVATRWYRAP ELCGSFFSKY TPAIDIWSIG CIFAELLTGR PLFPGKNVVH 300
    QLDIITDLLG TPSSETLSRI RNEKARRYLS TMRKKHAVPF SQKFRNTDPL 350
    ALRLLERLLA FDPKDRSSAE EALADPYFAS LANVEREPSR HPISKLEFEF 400
    ERRKLTKDDV RELIYREILE YHPQMLQEYM KGGEQISFLY PSGVDRFKRQ 450
    FAHLEENYSK GERGSPLQRK HASLPRERVG VSKDGYNQQN TNDQERSADS 500
    VARTTVSPPM SQDAQQHGSA GQNGVTSTDL SSRSYLKSAS ISASKCVAVK 550
    DNKEPEDDYI SEEMEGSVDG LSEQVSRMHS 580
    Length:580
    Mass (Da):65,945
    Last modified:June 13, 2006 - v2
    Checksum:i793AA85445857EF6
    GO
    Isoform 2 (identifier: Q5Z9J0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-74: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:506
    Mass (Da):57,946
    Checksum:iB05509010B2673C4
    GO

    Sequence cautioni

    The sequence AAF23902.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti196 – 1961Q → R in AK066531. (PubMed:12869764)Curated
    Sequence conflicti367 – 3671S → P in AAD52659. (PubMed:10624015)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7474Missing in isoform 2. 1 PublicationVSP_019264Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177392 mRNA. Translation: AAD52659.1.
    AP003621 Genomic DNA. Translation: BAD53616.1.
    AP003621 Genomic DNA. Translation: BAD53617.1.
    AK066531 mRNA. No translation available.
    AK067925 mRNA. No translation available.
    AF194415 mRNA. Translation: AAF23902.1. Different initiation.
    RefSeqiNP_001058530.1. NM_001065065.1. [Q5Z9J0-1]
    UniGeneiOs.7913.

    Genome annotation databases

    EnsemblPlantsiOS06T0708000-01; OS06T0708000-01; OS06G0708000. [Q5Z9J0-1]
    GeneIDi4342017.
    KEGGiosa:4342017.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF177392 mRNA. Translation: AAD52659.1 .
    AP003621 Genomic DNA. Translation: BAD53616.1 .
    AP003621 Genomic DNA. Translation: BAD53617.1 .
    AK066531 mRNA. No translation available.
    AK067925 mRNA. No translation available.
    AF194415 mRNA. Translation: AAF23902.1 . Different initiation.
    RefSeqi NP_001058530.1. NM_001065065.1. [Q5Z9J0-1 ]
    UniGenei Os.7913.

    3D structure databases

    ProteinModelPortali Q5Z9J0.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5Z9J0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0708000-01 ; OS06T0708000-01 ; OS06G0708000 . [Q5Z9J0-1 ]
    GeneIDi 4342017.
    KEGGi osa:4342017.

    Organism-specific databases

    Gramenei Q5Z9J0.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    OMAi FTHKFRN.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "BWMK1, a novel MAP kinase induced by fungal infection and mechanical wounding in rice."
      He C., Fong S.H.T., Yang D., Wang G.-L.
      Mol. Plant Microbe Interact. 12:1064-1073(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME REGULATION, INDUCTION, PHOSPHORYLATION.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Nipponbare.
    4. "BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor."
      Cheong Y.H., Moon B.C., Kim J.K., Kim C.Y., Kim M.C., Kim I.H., Park C.Y., Kim J.C., Park B.O., Koo S.C., Yoon H.W., Chung W.S., Lim C.O., Lee S.Y., Cho M.J.
      Plant Physiol. 132:1961-1972(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 37-580 (ISOFORM 1), FUNCTION, ENZYME REGULATION, SUBCELLULAR LOCATION, INDUCTION, INTERACTION WITH EREBP1.
    5. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
      Reyna N.S., Yang Y.
      Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, NOMENCLATURE.

    Entry informationi

    Entry nameiMPK12_ORYSJ
    AccessioniPrimary (citable) accession number: Q5Z9J0
    Secondary accession number(s): Q5Z9J1, Q9SE23, Q9SPF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3