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Q5Z8V9

- HEM2_ORYSJ

UniProt

Q5Z8V9 - HEM2_ORYSJ

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Protein
Delta-aminolevulinic acid dehydratase, chloroplastic
Gene
HEMB, Os06g0704600, LOC_Os06g49110, OsJ_22561, P0018H04.2
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei293 – 2931Schiff-base intermediate with substrate By similarity
Binding sitei303 – 3031Substrate 1 By similarity
Binding sitei315 – 3151Substrate 1 By similarity
Metal bindingi331 – 3311Magnesium By similarity
Active sitei346 – 3461Schiff-base intermediate with substrate By similarity
Binding sitei372 – 3721Substrate 2 By similarity
Binding sitei411 – 4111Substrate 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Ordered Locus Names:Os06g0704600, LOC_Os06g49110
ORF Names:OsJ_22561, P0018H04.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ5Z8V9.

Subcellular locationi

Plastidchloroplast Inferred

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4545Chloroplast Reviewed prediction
Add
BLAST
Chaini46 – 426381Delta-aminolevulinic acid dehydratase, chloroplastic
PRO_0000376065Add
BLAST

Proteomic databases

PaxDbiQ5Z8V9.
PRIDEiQ5Z8V9.

Interactioni

Subunit structurei

Homooctamer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5Z8V9.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0113.
HOGENOMiHOG000020323.
KOiK01698.
OMAiMTILTHR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5Z8V9-1 [UniParc]FASTAAdd to Basket

« Hide

MASTVSFSPA NVQMLQGRSC HGHAAFGGCS AVPRTGPRMR SVAVRVSSEQ    50
EAAPAVRAPS GRTIEECEAD AVAGRFPAPP PLVRPKAPEG TPQIRPLDLT 100
KRPRRNRRSP ALRAAFQETT ISPANLVLPL FIHEGEDDAP IGAMPGCYRL 150
GWRHGLLDEV YKSRDVGVNS FVLFPKVPDA LKSQSGDEAY NDNGLVPRTI 200
RLLKDKFPDI VVYTDVALDP YSSDGHDGIV REDGVIMNDE TVYQLCKQAV 250
SQARAGADVV SPSDMMDGRV GAIRAALDAE GFHDVSIMSY TAKYASSFYG 300
PFREALDSNP RFGDKKTYQM NPANYREALL ETAADEAEGA DILLVKPGLP 350
YLDVIRLLRD NSALPIAAYQ VSGEYSMIKA GGALNMIDEE KVMMESLMCL 400
RRAGADIILT YFARQAANVL CGMRSN 426
Length:426
Mass (Da):46,286
Last modified:November 23, 2004 - v1
Checksum:i04A43F6AE5E51DAF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003761 Genomic DNA. Translation: BAD53795.1.
AP008212 Genomic DNA. Translation: BAF20424.1.
CM000143 Genomic DNA. Translation: EAZ38208.1.
AK065784 mRNA. Translation: BAG89678.1.
AK119551 mRNA. Translation: BAG99687.1.
RefSeqiNP_001058510.1. NM_001065045.1.
UniGeneiOs.7836.

Genome annotation databases

EnsemblPlantsiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
GeneIDi4341997.
KEGGiosa:4341997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP003761 Genomic DNA. Translation: BAD53795.1 .
AP008212 Genomic DNA. Translation: BAF20424.1 .
CM000143 Genomic DNA. Translation: EAZ38208.1 .
AK065784 mRNA. Translation: BAG89678.1 .
AK119551 mRNA. Translation: BAG99687.1 .
RefSeqi NP_001058510.1. NM_001065045.1.
UniGenei Os.7836.

3D structure databases

ProteinModelPortali Q5Z8V9.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q5Z8V9.
PRIDEi Q5Z8V9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0704600-01 ; OS06T0704600-01 ; OS06G0704600 .
OS06T0704600-02 ; OS06T0704600-02 ; OS06G0704600 .
GeneIDi 4341997.
KEGGi osa:4341997.

Organism-specific databases

Gramenei Q5Z8V9.

Phylogenomic databases

eggNOGi COG0113.
HOGENOMi HOG000020323.
KOi K01698.
OMAi MTILTHR.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  3. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiHEM2_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z8V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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