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Q5Z8V9 (HEM2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic

Short name=ALADH
EC=4.2.1.24
Alternative name(s):
Porphobilinogen synthase
Gene names
Name:HEMB
Ordered Locus Names:Os06g0704600, LOC_Os06g49110
ORF Names:OsJ_22561, P0018H04.2
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activity

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactor

Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.

Subunit structure

Homooctamer By similarity.

Subcellular location

Plastidchloroplast Probable.

Sequence similarities

Belongs to the ALADH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4545Chloroplast Potential
Chain46 – 426381Delta-aminolevulinic acid dehydratase, chloroplastic
PRO_0000376065

Sites

Active site2931Schiff-base intermediate with substrate By similarity
Active site3461Schiff-base intermediate with substrate By similarity
Metal binding3311Magnesium By similarity
Binding site3031Substrate 1 By similarity
Binding site3151Substrate 1 By similarity
Binding site3721Substrate 2 By similarity
Binding site4111Substrate 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z8V9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 04A43F6AE5E51DAF

FASTA42646,286
        10         20         30         40         50         60 
MASTVSFSPA NVQMLQGRSC HGHAAFGGCS AVPRTGPRMR SVAVRVSSEQ EAAPAVRAPS 

        70         80         90        100        110        120 
GRTIEECEAD AVAGRFPAPP PLVRPKAPEG TPQIRPLDLT KRPRRNRRSP ALRAAFQETT 

       130        140        150        160        170        180 
ISPANLVLPL FIHEGEDDAP IGAMPGCYRL GWRHGLLDEV YKSRDVGVNS FVLFPKVPDA 

       190        200        210        220        230        240 
LKSQSGDEAY NDNGLVPRTI RLLKDKFPDI VVYTDVALDP YSSDGHDGIV REDGVIMNDE 

       250        260        270        280        290        300 
TVYQLCKQAV SQARAGADVV SPSDMMDGRV GAIRAALDAE GFHDVSIMSY TAKYASSFYG 

       310        320        330        340        350        360 
PFREALDSNP RFGDKKTYQM NPANYREALL ETAADEAEGA DILLVKPGLP YLDVIRLLRD 

       370        380        390        400        410        420 
NSALPIAAYQ VSGEYSMIKA GGALNMIDEE KVMMESLMCL RRAGADIILT YFARQAANVL 


CGMRSN 

« Hide

References

« Hide 'large scale' references
[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[3]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP003761 Genomic DNA. Translation: BAD53795.1.
AP008212 Genomic DNA. Translation: BAF20424.1.
CM000143 Genomic DNA. Translation: EAZ38208.1.
AK065784 mRNA. Translation: BAG89678.1.
AK119551 mRNA. Translation: BAG99687.1.
RefSeqNP_001058510.1. NM_001065045.1.
UniGeneOs.7836.

3D structure databases

ProteinModelPortalQ5Z8V9.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ5Z8V9.
PRIDEQ5Z8V9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
GeneID4341997.
KEGGosa:4341997.

Organism-specific databases

GrameneQ5Z8V9.

Phylogenomic databases

eggNOGCOG0113.
HOGENOMHOG000020323.
KOK01698.
OMAISYHAKE.
ProtClustDBCLSN2679842.

Enzyme and pathway databases

UniPathwayUPA00251; UER00318.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERPTHR11458. PTHR11458. 1 hit.
PfamPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSPR00144. DALDHYDRTASE.
SMARTSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM2_ORYSJ
AccessionPrimary (citable) accession number: Q5Z8V9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways