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Q5Z8V9

- HEM2_ORYSJ

UniProt

Q5Z8V9 - HEM2_ORYSJ

Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei293 – 2931Schiff-base intermediate with substrateBy similarity
    Binding sitei303 – 3031Substrate 1By similarity
    Binding sitei315 – 3151Substrate 1By similarity
    Metal bindingi331 – 3311MagnesiumBy similarity
    Active sitei346 – 3461Schiff-base intermediate with substrateBy similarity
    Binding sitei372 – 3721Substrate 2By similarity
    Binding sitei411 – 4111Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-KW
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:HEMB
    Ordered Locus Names:Os06g0704600, LOC_Os06g49110
    ORF Names:OsJ_22561, P0018H04.2
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ5Z8V9.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast stroma Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4545ChloroplastSequence AnalysisAdd
    BLAST
    Chaini46 – 426381Delta-aminolevulinic acid dehydratase, chloroplasticPRO_0000376065Add
    BLAST

    Proteomic databases

    PaxDbiQ5Z8V9.
    PRIDEiQ5Z8V9.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5Z8V9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0113.
    HOGENOMiHOG000020323.
    KOiK01698.
    OMAiMTILTHR.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5Z8V9-1 [UniParc]FASTAAdd to Basket

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    MASTVSFSPA NVQMLQGRSC HGHAAFGGCS AVPRTGPRMR SVAVRVSSEQ    50
    EAAPAVRAPS GRTIEECEAD AVAGRFPAPP PLVRPKAPEG TPQIRPLDLT 100
    KRPRRNRRSP ALRAAFQETT ISPANLVLPL FIHEGEDDAP IGAMPGCYRL 150
    GWRHGLLDEV YKSRDVGVNS FVLFPKVPDA LKSQSGDEAY NDNGLVPRTI 200
    RLLKDKFPDI VVYTDVALDP YSSDGHDGIV REDGVIMNDE TVYQLCKQAV 250
    SQARAGADVV SPSDMMDGRV GAIRAALDAE GFHDVSIMSY TAKYASSFYG 300
    PFREALDSNP RFGDKKTYQM NPANYREALL ETAADEAEGA DILLVKPGLP 350
    YLDVIRLLRD NSALPIAAYQ VSGEYSMIKA GGALNMIDEE KVMMESLMCL 400
    RRAGADIILT YFARQAANVL CGMRSN 426
    Length:426
    Mass (Da):46,286
    Last modified:November 23, 2004 - v1
    Checksum:i04A43F6AE5E51DAF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003761 Genomic DNA. Translation: BAD53795.1.
    AP008212 Genomic DNA. Translation: BAF20424.1.
    CM000143 Genomic DNA. Translation: EAZ38208.1.
    AK065784 mRNA. Translation: BAG89678.1.
    AK119551 mRNA. Translation: BAG99687.1.
    RefSeqiNP_001058510.1. NM_001065045.1.
    UniGeneiOs.7836.

    Genome annotation databases

    EnsemblPlantsiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
    OS06T0704600-02; OS06T0704600-02; OS06G0704600.
    GeneIDi4341997.
    KEGGiosa:4341997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003761 Genomic DNA. Translation: BAD53795.1 .
    AP008212 Genomic DNA. Translation: BAF20424.1 .
    CM000143 Genomic DNA. Translation: EAZ38208.1 .
    AK065784 mRNA. Translation: BAG89678.1 .
    AK119551 mRNA. Translation: BAG99687.1 .
    RefSeqi NP_001058510.1. NM_001065045.1.
    UniGenei Os.7836.

    3D structure databases

    ProteinModelPortali Q5Z8V9.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q5Z8V9.
    PRIDEi Q5Z8V9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0704600-01 ; OS06T0704600-01 ; OS06G0704600 .
    OS06T0704600-02 ; OS06T0704600-02 ; OS06G0704600 .
    GeneIDi 4341997.
    KEGGi osa:4341997.

    Organism-specific databases

    Gramenei Q5Z8V9.

    Phylogenomic databases

    eggNOGi COG0113.
    HOGENOMi HOG000020323.
    KOi K01698.
    OMAi MTILTHR.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    3. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiHEM2_ORYSJ
    AccessioniPrimary (citable) accession number: Q5Z8V9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3