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Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase, chloroplastic (HEMB)
  2. Porphobilinogen deaminase, chloroplastic (HEMC)
  3. Uroporphyrinogen-III synthase, chloroplastic (UROS)
  4. Uroporphyrinogen decarboxylase 2, chloroplastic (Os03g0337600), Uroporphyrinogen decarboxylase 1, chloroplastic (Os01g0622300)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei293Schiff-base intermediate with substrateBy similarity1
Binding sitei303Substrate 1By similarity1
Binding sitei315Substrate 1By similarity1
Metal bindingi331MagnesiumBy similarity1
Active sitei346Schiff-base intermediate with substrateBy similarity1
Binding sitei372Substrate 2By similarity1
Binding sitei411Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-OSA-189451. Heme biosynthesis.
R-OSA-6798695. Neutrophil degranulation.
UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Ordered Locus Names:Os06g0704600, LOC_Os06g49110
ORF Names:OsJ_22561, P0018H04.2
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 45ChloroplastSequence analysisAdd BLAST45
ChainiPRO_000037606546 – 426Delta-aminolevulinic acid dehydratase, chloroplasticAdd BLAST381

Proteomic databases

PaxDbiQ5Z8V9.
PRIDEiQ5Z8V9.

Expressioni

Gene expression databases

GenevisibleiQ5Z8V9. OS.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os06g49110.1.

Structurei

3D structure databases

ProteinModelPortaliQ5Z8V9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2794. Eukaryota.
COG0113. LUCA.
HOGENOMiHOG000020323.
InParanoidiQ5Z8V9.
KOiK01698.
OMAiMDPANSN.
OrthoDBiEOG09360FP1.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5Z8V9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTVSFSPA NVQMLQGRSC HGHAAFGGCS AVPRTGPRMR SVAVRVSSEQ
60 70 80 90 100
EAAPAVRAPS GRTIEECEAD AVAGRFPAPP PLVRPKAPEG TPQIRPLDLT
110 120 130 140 150
KRPRRNRRSP ALRAAFQETT ISPANLVLPL FIHEGEDDAP IGAMPGCYRL
160 170 180 190 200
GWRHGLLDEV YKSRDVGVNS FVLFPKVPDA LKSQSGDEAY NDNGLVPRTI
210 220 230 240 250
RLLKDKFPDI VVYTDVALDP YSSDGHDGIV REDGVIMNDE TVYQLCKQAV
260 270 280 290 300
SQARAGADVV SPSDMMDGRV GAIRAALDAE GFHDVSIMSY TAKYASSFYG
310 320 330 340 350
PFREALDSNP RFGDKKTYQM NPANYREALL ETAADEAEGA DILLVKPGLP
360 370 380 390 400
YLDVIRLLRD NSALPIAAYQ VSGEYSMIKA GGALNMIDEE KVMMESLMCL
410 420
RRAGADIILT YFARQAANVL CGMRSN
Length:426
Mass (Da):46,286
Last modified:November 23, 2004 - v1
Checksum:i04A43F6AE5E51DAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003761 Genomic DNA. Translation: BAD53795.1.
AP008212 Genomic DNA. Translation: BAF20424.1.
AP014962 Genomic DNA. Translation: BAS99386.1.
CM000143 Genomic DNA. Translation: EAZ38208.1.
AK065784 mRNA. Translation: BAG89678.1.
AK119551 mRNA. Translation: BAG99687.1.
RefSeqiXP_015643005.1. XM_015787519.1.
XP_015643007.1. XM_015787521.1.
XP_015643008.1. XM_015787522.1.
XP_015643009.1. XM_015787523.1.
UniGeneiOs.7836.

Genome annotation databases

EnsemblPlantsiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
GeneIDi4341997.
GrameneiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
KEGGiosa:4341997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP003761 Genomic DNA. Translation: BAD53795.1.
AP008212 Genomic DNA. Translation: BAF20424.1.
AP014962 Genomic DNA. Translation: BAS99386.1.
CM000143 Genomic DNA. Translation: EAZ38208.1.
AK065784 mRNA. Translation: BAG89678.1.
AK119551 mRNA. Translation: BAG99687.1.
RefSeqiXP_015643005.1. XM_015787519.1.
XP_015643007.1. XM_015787521.1.
XP_015643008.1. XM_015787522.1.
XP_015643009.1. XM_015787523.1.
UniGeneiOs.7836.

3D structure databases

ProteinModelPortaliQ5Z8V9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g49110.1.

Proteomic databases

PaxDbiQ5Z8V9.
PRIDEiQ5Z8V9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
GeneIDi4341997.
GrameneiOS06T0704600-01; OS06T0704600-01; OS06G0704600.
OS06T0704600-02; OS06T0704600-02; OS06G0704600.
KEGGiosa:4341997.

Phylogenomic databases

eggNOGiKOG2794. Eukaryota.
COG0113. LUCA.
HOGENOMiHOG000020323.
InParanoidiQ5Z8V9.
KOiK01698.
OMAiMDPANSN.
OrthoDBiEOG09360FP1.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.
ReactomeiR-OSA-189451. Heme biosynthesis.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

GenevisibleiQ5Z8V9. OS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR001731. ALAD.
IPR030656. ALAD_AS.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEM2_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z8V9
Secondary accession number(s): A0A0P0X108
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.