Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5Z8T3 (MIOX_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable inositol oxygenase

EC=1.13.99.1
Alternative name(s):
Myo-inositol oxygenase
Short name=MI oxygenase
Gene names
Ordered Locus Names:Os06g0561000, LOC_Os06g36560
ORF Names:P0456F09.5, P0528E12.30
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis By similarity.

Catalytic activity

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactor

Binds 2 iron ions per subunit By similarity.

Pathway

Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the myo-inositol oxygenase family.

Ontologies

Keywords
   Biological processAscorbate biosynthesis
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-ascorbic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

inositol catabolic process

Inferred from electronic annotation. Source: InterPro

syncytium formation

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioninositol oxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 308308Probable inositol oxygenase
PRO_0000079158

Regions

Region106 – 1083Substrate binding By similarity
Region165 – 1662Substrate binding By similarity
Region243 – 2442Substrate binding By similarity

Sites

Metal binding1191Iron 1 By similarity
Metal binding1441Iron 1 By similarity
Metal binding1451Iron 1 By similarity
Metal binding1451Iron 2 By similarity
Metal binding2171Iron 2 By similarity
Metal binding2431Iron 2 By similarity
Metal binding2761Iron 1 By similarity
Binding site491Substrate By similarity
Binding site1481Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z8T3 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 7612DA5BFA7DE5FF

FASTA30835,805
        10         20         30         40         50         60 
MTITIEQPHL DAIADRKVAG GGGGDNAAEL VLDGGFVVPD SNAFGNAFRN YEAESERKET 

        70         80         90        100        110        120 
VEEFYRVNHI NQTYDFVRRM REEYGRVDKT EMGIWECIEL LNEFIDDSDP DLDMPQIEHL 

       130        140        150        160        170        180 
LQTAEAIRKD FPDEDWLHLT GLIHDLGKVL LHPSFGELPQ WSVVGDTFPV GCAFDECNVH 

       190        200        210        220        230        240 
FKYFKENPDY LNPKLNTKFG AYSEGCGLDN VLMSWGHDDY MYLVAKENKT TLPSAGLFII 

       250        260        270        280        290        300 
RYHSFYPLHK HGAYMHLMND EDKENLKWLR VFNKYDLYSK SNERIDVEKV KPYYMSLIEK 


YFPAKLRW 

« Hide

References

[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP003713 Genomic DNA. Translation: BAD53740.1.
AP003762 Genomic DNA. Translation: BAD53821.1.
AK068862 mRNA. No translation available.
RefSeqNP_001057871.1. NM_001064406.1.
UniGeneOs.6345.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0561000-01; OS06T0561000-01; OS06G0561000.
GeneID4341305.
KEGGosa:4341305.

Organism-specific databases

GrameneQ5Z8T3.

Phylogenomic databases

eggNOGNOG135479.
KOK00469.
OMANFYREQH.
ProtClustDBCLSN2687348.

Enzyme and pathway databases

UniPathwayUPA00111; UER00527.

Family and domain databases

InterProIPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERPTHR12588. PTHR12588. 1 hit.
PfamPF05153. DUF706. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIOX_ORYSJ
AccessionPrimary (citable) accession number: Q5Z8T3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways