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Q5Z8T3

- MIOX_ORYSJ

UniProt

Q5Z8T3 - MIOX_ORYSJ

Protein

Probable inositol oxygenase

Gene

Os06g0561000

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis By similarity.By similarity

    Catalytic activityi

    Myo-inositol + O2 = D-glucuronate + H2O.

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491SubstrateBy similarity
    Metal bindingi119 – 1191Iron 1By similarity
    Metal bindingi144 – 1441Iron 1By similarity
    Metal bindingi145 – 1451Iron 1By similarity
    Metal bindingi145 – 1451Iron 2By similarity
    Binding sitei148 – 1481SubstrateBy similarity
    Metal bindingi217 – 2171Iron 2By similarity
    Metal bindingi243 – 2431Iron 2By similarity
    Metal bindingi276 – 2761Iron 1By similarity

    GO - Molecular functioni

    1. inositol oxygenase activity Source: UniProtKB-EC
    2. iron ion binding Source: InterPro

    GO - Biological processi

    1. inositol catabolic process Source: InterPro
    2. L-ascorbic acid biosynthetic process Source: UniProtKB-KW
    3. syncytium formation Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Ascorbate biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00111; UER00527.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable inositol oxygenase (EC:1.13.99.1)
    Alternative name(s):
    Myo-inositol oxygenase
    Short name:
    MI oxygenase
    Gene namesi
    Ordered Locus Names:Os06g0561000, LOC_Os06g36560
    ORF Names:P0456F09.5, P0528E12.30
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ5Z8T3.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 308308Probable inositol oxygenasePRO_0000079158Add
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 1083Substrate bindingBy similarity
    Regioni165 – 1662Substrate bindingBy similarity
    Regioni243 – 2442Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the myo-inositol oxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG135479.
    KOiK00469.
    OMAiNFYREQH.

    Family and domain databases

    InterProiIPR007828. Inositol_oxygenase.
    [Graphical view]
    PANTHERiPTHR12588. PTHR12588. 1 hit.
    PfamiPF05153. DUF706. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5Z8T3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTITIEQPHL DAIADRKVAG GGGGDNAAEL VLDGGFVVPD SNAFGNAFRN    50
    YEAESERKET VEEFYRVNHI NQTYDFVRRM REEYGRVDKT EMGIWECIEL 100
    LNEFIDDSDP DLDMPQIEHL LQTAEAIRKD FPDEDWLHLT GLIHDLGKVL 150
    LHPSFGELPQ WSVVGDTFPV GCAFDECNVH FKYFKENPDY LNPKLNTKFG 200
    AYSEGCGLDN VLMSWGHDDY MYLVAKENKT TLPSAGLFII RYHSFYPLHK 250
    HGAYMHLMND EDKENLKWLR VFNKYDLYSK SNERIDVEKV KPYYMSLIEK 300
    YFPAKLRW 308
    Length:308
    Mass (Da):35,805
    Last modified:November 23, 2004 - v1
    Checksum:i7612DA5BFA7DE5FF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003713 Genomic DNA. Translation: BAD53740.1.
    AP003762 Genomic DNA. Translation: BAD53821.1.
    AK068862 mRNA. No translation available.
    RefSeqiNP_001057871.1. NM_001064406.1.
    UniGeneiOs.6345.

    Genome annotation databases

    EnsemblPlantsiOS06T0561000-01; OS06T0561000-01; OS06G0561000.
    GeneIDi4341305.
    KEGGiosa:4341305.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP003713 Genomic DNA. Translation: BAD53740.1 .
    AP003762 Genomic DNA. Translation: BAD53821.1 .
    AK068862 mRNA. No translation available.
    RefSeqi NP_001057871.1. NM_001064406.1.
    UniGenei Os.6345.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0561000-01 ; OS06T0561000-01 ; OS06G0561000 .
    GeneIDi 4341305.
    KEGGi osa:4341305.

    Organism-specific databases

    Gramenei Q5Z8T3.

    Phylogenomic databases

    eggNOGi NOG135479.
    KOi K00469.
    OMAi NFYREQH.

    Enzyme and pathway databases

    UniPathwayi UPA00111 ; UER00527 .

    Family and domain databases

    InterProi IPR007828. Inositol_oxygenase.
    [Graphical view ]
    PANTHERi PTHR12588. PTHR12588. 1 hit.
    Pfami PF05153. DUF706. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiMIOX_ORYSJ
    AccessioniPrimary (citable) accession number: Q5Z8T3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 8, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 60 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3