Reviewed, UniProtKB/Swiss-Prot Q5Z8T3 (MIOX_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable inositol oxygenase
    EC=1.13.99.1
Alternative name(s):
    Myo-inositol oxygenase
      Short name=MI oxygenase

Gene names

Ordered Locus Names:	Os06g0561000, LOC_Os06g36560
ORF Names:	P0456F09.5, P0528E12.30

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the biosynthesis of UDP-glucuronic acid (UDP-GlcA), providing nucleotide sugars for cell-wall polymers. May be also involved in plant ascorbate biosynthesis By similarity.
Catalytic activity	Myo-inositol + O(2) = D-glucuronate + H(2)O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the myo-inositol oxygenase family.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW inositol catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	inositol oxygenase activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Probable inositol oxygenase		PRO_0000079158
Sites
	Metal binding	119	1	Iron 1 By similarity
	Metal binding	144	1	Iron 1 By similarity
	Metal binding	145	1	Iron 1 By similarity
	Metal binding	145	1	Iron 2 By similarity
	Metal binding	217	1	Iron 2 By similarity
	Metal binding	243	1	Iron 2 By similarity
	Metal binding	276	1	Iron 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z8T3-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 7612DA5BFA7DE5FF	FASTA	308	35,805
	10 20 30 40 50 60 MTITIEQPHL DAIADRKVAG GGGGDNAAEL VLDGGFVVPD SNAFGNAFRN YEAESERKET 70 80 90 100 110 120 VEEFYRVNHI NQTYDFVRRM REEYGRVDKT EMGIWECIEL LNEFIDDSDP DLDMPQIEHL 130 140 150 160 170 180 LQTAEAIRKD FPDEDWLHLT GLIHDLGKVL LHPSFGELPQ WSVVGDTFPV GCAFDECNVH 190 200 210 220 230 240 FKYFKENPDY LNPKLNTKFG AYSEGCGLDN VLMSWGHDDY MYLVAKENKT TLPSAGLFII 250 260 270 280 290 300 RYHSFYPLHK HGAYMHLMND EDKENLKWLR VFNKYDLYSK SNERIDVEKV KPYYMSLIEK YFPAKLRW

References

[1]	"Oryza sativa nipponbare chromosome 6 genomic DNA sequence." The international rice genome sequencing project (IRGSP) consortium Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP003713 Genomic DNA. Translation: BAD53740.1. AP003762 Genomic DNA. Translation: BAD53821.1. AK068862 mRNA. No translation available.
RefSeq	NP_001057871.1.
UniGene	Os.6345
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4341305.
KEGG	osa:4341305.
Organism-specific databases
Gramene	Q5Z8T3.
Family and domain databases
InterPro	IPR007828. DUF706. [Graphical view]
PANTHER	PTHR12588. DUF706. 1 hit.
Pfam	PF05153. DUF706. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIOX_ORYSJ

Accession

Primary (citable) accession number: Q5Z8T3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	November 23, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)