Q5Z8Q9 (PRP2A_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly [ADP-ribose] polymerase 2-A
Short name=PARP-2-A
EC=2.4.2.30

Alternative name(s):
NAD(+) ADP-ribosyltransferase 2-A
Short name=ADPRT-2-A
Poly[ADP-ribose] synthase 2-A

Gene names

Name:	PARP2-A
Ordered Locus Names:	Os01g0351200, LOC_Os01g24940
ORF Names:	B1051E10.55, P0463A02.25

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 SAP domains.
Sequence caution	The sequence BAD52929.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAD53855.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAF04898.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding NAD
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Poly [ADP-ribose] polymerase 2-A		PRO_0000260503
Regions
	Domain	2 – 36	35	SAP 1
	Domain	91 – 125	35	SAP 2
	Domain	308 – 426	119	PARP alpha-helical
	Domain	434 – 660	227	PARP catalytic
	Motif	69 – 75	7	Nuclear localization signal Potential
	Compositional bias	39 – 43	5	Poly-Glu

Sequences

	Sequence		Length	Mass (Da)
	Q5Z8Q9 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 94FA8F38B72470A9	FASTA	660	74,297
	10 20 30 40 50 60 MSARLRVEEL RAELQRRGLD ASGNKPVLVR RLDAAIRKEE EEEAAVSAAA KEEADAGGVV 70 80 90 100 110 120 DGEGNGEDKR KRKRRGDGED VDNSESDAAK LEGMSYRELQ ALAKSRGLAA NGSKKEVIER 130 140 150 160 170 180 LLCAPSDTDG GVQDKKKIAK GFADGDDRVE ECRKEKIVTA TRKGAAVLDQ HIPDHIKMTY 190 200 210 220 230 240 HVLQVWFLLK GDEIYDATMN QTNVGDNNNK FYIIQALESD AGGSFMVYNR WGRVGARGQD 250 260 270 280 290 300 KLHGPFSSRE QAIYEFEGKF HGKTNNHWSD RKSFECYARK YTWLEMDYGE ADRETNKKVS 310 320 330 340 350 360 PSTDQIKETK LETRIASFIS LICNISMMKQ QMVEIGYNSD KLPLGKLSKS TIFKGYDVLK 370 380 390 400 410 420 RISNVISRAD RRQLEQLTGE FYTVIPHDFG FKKMREFIID TPQKLKAKLE MVEALGEIEI 430 440 450 460 470 480 ATKLLEDDST DQDDPLYARY KQLSCDFTPL EVGSEEYSMI KTYLANTHGK THTSYTVDVV 490 500 510 520 530 540 QIFKVSRHGE MERFQKFATA GNRMLLWHGS RLTNWAGILS QGLRIAPPEA PVTGYMFGKG 550 560 570 580 590 600 VYFADMFSKS ANYCYASEAC RSGVLLLCEV ALGEMNELLN ADYDANNLPK GKLSTKGVGQ 610 620 630 640 650 660 TEPNTAESKI TDDGVVVPLG KPKAEPSKRG SLLYNEFIVY NVDQIRMRYV LHVSFNFKKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003258 Genomic DNA. Translation: BAD52929.1. Sequence problems. AP003764 Genomic DNA. Translation: BAD53855.1. Sequence problems. AP008207 Genomic DNA. Translation: BAF04898.2. Sequence problems.
RefSeq	NP_001042984.2. NM_001049519.2.
UniGene	Os.24852.
3D structure databases
HSSP	HSSP built from PDB template 1GS0 based on UniProtKB O88554.
ProteinModelPortal	Q5Z8Q9.
SMR	Q5Z8Q9. Positions 3-40.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os01g24940.1; LOC_Os01g24940.1; LOC_Os01g24940.
GeneID	4326479.
KEGG	osa:4326479.
Organism-specific databases
Gramene	Q5Z8Q9.
Phylogenomic databases
GeneTree	EPGT00070000029473.
OMA	CICDEDV.
PhylomeDB	Q5Z8Q9.
Family and domain databases
InterPro	IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004102. Poly(ADP-ribose)pol_reg_dom. IPR003034. SAP_DNA-bd. IPR008893. WGR_domain. [Graphical view]
Gene3D	G3DSA:1.10.720.30. G3DSA:1.10.720.30. 2 hits. G3DSA:2.20.140.10. G3DSA:2.20.140.10. 1 hit. G3DSA:1.20.142.10. PARP_reg. 1 hit.
KO	K10798.
Pfam	PF00644. PARP. 1 hit. PF02877. PARP_reg. 1 hit. PF02037. SAP. 2 hits. PF05406. WGR. 1 hit. [Graphical view]
SMART	SM00513. SAP. 2 hits. SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF47587. PARP_reg. 1 hit. SSF142921. SSF142921. 1 hit.
PROSITE	PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50800. SAP. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRP2A_ORYSJ

Accession

Primary (citable) accession number: Q5Z8Q9
Secondary accession number(s): Q0JMY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	December 14, 2011
This is version 50 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program