Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5Z859

- MPK4_ORYSJ

UniProt

Q5Z859 - MPK4_ORYSJ

Protein

Mitogen-activated protein kinase 4

Gene

MPK4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activated by threonine and tyrosine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ATPPROSITE-ProRule annotation
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. response to hydrogen peroxide Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 4 (EC:2.7.11.24)
    Short name:
    MAP kinase 4
    Alternative name(s):
    Multiple stress-responsive MAP kinase 3
    OsMAP2
    OsMSRMK3
    Gene namesi
    Name:MPK4
    Synonyms:MAP2, MSRMK3
    Ordered Locus Names:Os06g0699400, LOC_Os06g48590
    ORF Names:P0468G03.11
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 6

    Organism-specific databases

    GrameneiQ5Z859.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Mitogen-activated protein kinase 4PRO_0000239747Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Expressed in leaves and panicles.1 Publication

    Inductioni

    By stresses, hormones, heavy metals, phosphatase inhibitors and infection with rice blast fungus (M.grisea). Down-regulated by drought, high/low temperature and UV-C.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ5Z859.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK04371.
    OMAiMYPQAHP.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5Z859-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMMVDPPNG MGNQGKHYYT MWQTLFEIDT KYVPIKPIGR GAYGIVCSSI    50
    NRATNEKVAI KKINNVFDNR VDALRTLREL KLLRHLRHEN VIALKDIMMP 100
    VHRRSFKDVY LVYELMDTDL HQIIKSSQPL SNDHCQYFLF QLLRGLKYLH 150
    SAGILHRDLK PGNLLVNANC DLKICDFGLA RTNNTKGQFM TEYVVTRWYR 200
    APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFPGTECL NQLKLIVNVL 250
    GTMSEADIEF IDNPKARKYI KTLPYTPGIP LTSMYPQAHP LAIDLLQKML 300
    VFDPSKRISV TEALEHPYMS PLYDPSANPP AQVPIDLDID ENLGVDMIRE 350
    MMWQEMLHYH PEVVAGVNM 369
    Length:369
    Mass (Da):42,244
    Last modified:November 23, 2004 - v1
    Checksum:iD21EBE45865CE08B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21A → V(PubMed:12507518)Curated
    Sequence conflicti2 – 21A → V1 PublicationCurated
    Sequence conflicti32 – 321Y → V in CAB61889. 1 PublicationCurated
    Sequence conflicti331 – 3311A → V in CAB61889. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF216316 mRNA. Translation: AAG40580.1.
    AJ512642 mRNA. Translation: CAD54741.1.
    AJ251330 mRNA. Translation: CAB61889.1.
    AP004278 Genomic DNA. Translation: BAD53997.1.
    RefSeqiNP_001058471.1. NM_001065006.1.
    UniGeneiOs.4602.

    Genome annotation databases

    EnsemblPlantsiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
    GeneIDi4341956.
    KEGGiosa:4341956.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF216316 mRNA. Translation: AAG40580.1 .
    AJ512642 mRNA. Translation: CAD54741.1 .
    AJ251330 mRNA. Translation: CAB61889.1 .
    AP004278 Genomic DNA. Translation: BAD53997.1 .
    RefSeqi NP_001058471.1. NM_001065006.1.
    UniGenei Os.4602.

    3D structure databases

    ProteinModelPortali Q5Z859.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS06T0699400-01 ; OS06T0699400-01 ; OS06G0699400 .
    GeneIDi 4341956.
    KEGGi osa:4341956.

    Organism-specific databases

    Gramenei Q5Z859.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K04371.
    OMAi MYPQAHP.

    Enzyme and pathway databases

    Reactomei REACT_205014. CREB phosphorylation through the activation of Ras.
    REACT_214549. Signalling to ERK5.
    REACT_215626. ERK2 activation.
    REACT_221522. ERKs are inactivated.
    REACT_221537. ERK1 activation.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
      Wen J.-Q., Oono K., Imai R.
      Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
      Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
      Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    3. "Molecular cloning and expression of a MAP kinase homologue from rice."
      Huang H.-J., Fu S.-F., Tai Y.-H., Huang D.-D., Kuo T.T.
      Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
      Reyna N.S., Yang Y.
      Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, NOMENCLATURE.

    Entry informationi

    Entry nameiMPK4_ORYSJ
    AccessioniPrimary (citable) accession number: Q5Z859
    Secondary accession number(s): Q8H0P1, Q9FQM2, Q9SMA9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3