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Protein

Mitogen-activated protein kinase 4

Gene

MPK4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 4 (EC:2.7.11.24)
Short name:
MAP kinase 4
Alternative name(s):
Multiple stress-responsive MAP kinase 3
OsMAP2
OsMSRMK3
Gene namesi
Name:MPK4
Synonyms:MAP2, MSRMK3
Ordered Locus Names:Os06g0699400, LOC_Os06g48590
ORF Names:OsJ_22520Imported, P0468G03.11
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002397471 – 369Mitogen-activated protein kinase 4Add BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5Z859.

Expressioni

Tissue specificityi

Expressed in leaves and panicles.1 Publication

Inductioni

By stresses, hormones, heavy metals, phosphatase inhibitors and infection with rice blast fungus (M.grisea). Down-regulated by drought, high/low temperature and UV-C.2 Publications

Gene expression databases

GenevisibleiQ5Z859. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g48590.1.

Structurei

3D structure databases

ProteinModelPortaliQ5Z859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ5Z859.
KOiK20535.
OMAiQEMIHYH.
OrthoDBiEOG09360CTC.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5Z859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMMVDPPNG MGNQGKHYYT MWQTLFEIDT KYVPIKPIGR GAYGIVCSSI
60 70 80 90 100
NRATNEKVAI KKINNVFDNR VDALRTLREL KLLRHLRHEN VIALKDIMMP
110 120 130 140 150
VHRRSFKDVY LVYELMDTDL HQIIKSSQPL SNDHCQYFLF QLLRGLKYLH
160 170 180 190 200
SAGILHRDLK PGNLLVNANC DLKICDFGLA RTNNTKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFPGTECL NQLKLIVNVL
260 270 280 290 300
GTMSEADIEF IDNPKARKYI KTLPYTPGIP LTSMYPQAHP LAIDLLQKML
310 320 330 340 350
VFDPSKRISV TEALEHPYMS PLYDPSANPP AQVPIDLDID ENLGVDMIRE
360
MMWQEMLHYH PEVVAGVNM
Length:369
Mass (Da):42,244
Last modified:November 23, 2004 - v1
Checksum:iD21EBE45865CE08B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → V (PubMed:12507518).Curated1
Sequence conflicti2A → V (Ref. 3) Curated1
Sequence conflicti32Y → V in CAB61889 (Ref. 3) Curated1
Sequence conflicti331A → V in CAB61889 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216316 mRNA. Translation: AAG40580.1.
AJ512642 mRNA. Translation: CAD54741.1.
AJ251330 mRNA. Translation: CAB61889.1.
AP004278 Genomic DNA. Translation: BAD53997.1.
AP008212 Genomic DNA. Translation: BAF20385.1.
AP014962 Genomic DNA. Translation: BAS99326.1.
CM000143 Genomic DNA. Translation: EAZ38166.1.
AK071376 mRNA. Translation: BAG92461.1.
RefSeqiXP_015643564.1. XM_015788078.1.
XP_015643565.1. XM_015788079.1.
XP_015643566.1. XM_015788080.1.
UniGeneiOs.4602.

Genome annotation databases

EnsemblPlantsiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
GeneIDi4341956.
GrameneiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
KEGGiosa:4341956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216316 mRNA. Translation: AAG40580.1.
AJ512642 mRNA. Translation: CAD54741.1.
AJ251330 mRNA. Translation: CAB61889.1.
AP004278 Genomic DNA. Translation: BAD53997.1.
AP008212 Genomic DNA. Translation: BAF20385.1.
AP014962 Genomic DNA. Translation: BAS99326.1.
CM000143 Genomic DNA. Translation: EAZ38166.1.
AK071376 mRNA. Translation: BAG92461.1.
RefSeqiXP_015643564.1. XM_015788078.1.
XP_015643565.1. XM_015788079.1.
XP_015643566.1. XM_015788080.1.
UniGeneiOs.4602.

3D structure databases

ProteinModelPortaliQ5Z859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g48590.1.

Proteomic databases

PaxDbiQ5Z859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
GeneIDi4341956.
GrameneiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
KEGGiosa:4341956.

Phylogenomic databases

eggNOGiKOG0660. Eukaryota.
ENOG410XNY0. LUCA.
HOGENOMiHOG000233024.
InParanoidiQ5Z859.
KOiK20535.
OMAiQEMIHYH.
OrthoDBiEOG09360CTC.

Enzyme and pathway databases

ReactomeiR-OSA-110056. MAPK3 (ERK1) activation.
R-OSA-112409. RAF-independent MAPK1/3 activation.
R-OSA-112411. MAPK1 (ERK2) activation.
R-OSA-198753. ERK/MAPK targets.
R-OSA-198765. Signalling to ERK5.
R-OSA-202670. ERKs are inactivated.
R-OSA-3371453. Regulation of HSF1-mediated heat shock response.
R-OSA-442742. CREB phosphorylation through the activation of Ras.
R-OSA-445144. Signal transduction by L1.
R-OSA-5673001. RAF/MAP kinase cascade.
R-OSA-5674135. MAP2K and MAPK activation.
R-OSA-5674499. Negative feedback regulation of MAPK pathway.
R-OSA-6798695. Neutrophil degranulation.

Gene expression databases

GenevisibleiQ5Z859. OS.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK4_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z859
Secondary accession number(s): Q0D9T8
, Q8H0P1, Q9FQM2, Q9SMA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 23, 2004
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.