Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5Z859

- MPK4_ORYSJ

UniProt

Q5Z859 - MPK4_ORYSJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Mitogen-activated protein kinase 4

Gene

MPK4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATPPROSITE-ProRule annotation
Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203586. ERK/MAPK targets.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.
REACT_224658. Regulation of HSF1-mediated heat shock response.
REACT_233484. Signal transduction by L1.
REACT_248492. ISG15 antiviral mechanism.
REACT_261734. Signaling by FGFR.
REACT_268490. Signalling to ERK5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 4 (EC:2.7.11.24)
Short name:
MAP kinase 4
Alternative name(s):
Multiple stress-responsive MAP kinase 3
OsMAP2
OsMSRMK3
Gene namesi
Name:MPK4
Synonyms:MAP2, MSRMK3
Ordered Locus Names:Os06g0699400, LOC_Os06g48590
ORF Names:P0468G03.11
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 6

Organism-specific databases

GrameneiQ5Z859.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Mitogen-activated protein kinase 4PRO_0000239747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911PhosphothreonineBy similarity
Modified residuei193 – 1931PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in leaves and panicles.1 Publication

Inductioni

By stresses, hormones, heavy metals, phosphatase inhibitors and infection with rice blast fungus (M.grisea). Down-regulated by drought, high/low temperature and UV-C.2 Publications

Gene expression databases

ExpressionAtlasiQ5Z859. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ5Z859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5Z859.
KOiK04371.
OMAiMYPQAHP.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5Z859-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAMMVDPPNG MGNQGKHYYT MWQTLFEIDT KYVPIKPIGR GAYGIVCSSI
60 70 80 90 100
NRATNEKVAI KKINNVFDNR VDALRTLREL KLLRHLRHEN VIALKDIMMP
110 120 130 140 150
VHRRSFKDVY LVYELMDTDL HQIIKSSQPL SNDHCQYFLF QLLRGLKYLH
160 170 180 190 200
SAGILHRDLK PGNLLVNANC DLKICDFGLA RTNNTKGQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFPGTECL NQLKLIVNVL
260 270 280 290 300
GTMSEADIEF IDNPKARKYI KTLPYTPGIP LTSMYPQAHP LAIDLLQKML
310 320 330 340 350
VFDPSKRISV TEALEHPYMS PLYDPSANPP AQVPIDLDID ENLGVDMIRE
360
MMWQEMLHYH PEVVAGVNM
Length:369
Mass (Da):42,244
Last modified:November 23, 2004 - v1
Checksum:iD21EBE45865CE08B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → V(PubMed:12507518)Curated
Sequence conflicti2 – 21A → V1 PublicationCurated
Sequence conflicti32 – 321Y → V in CAB61889. 1 PublicationCurated
Sequence conflicti331 – 3311A → V in CAB61889. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216316 mRNA. Translation: AAG40580.1.
AJ512642 mRNA. Translation: CAD54741.1.
AJ251330 mRNA. Translation: CAB61889.1.
AP004278 Genomic DNA. Translation: BAD53997.1.
RefSeqiNP_001058471.1. NM_001065006.1.
UniGeneiOs.4602.

Genome annotation databases

EnsemblPlantsiOS06T0699400-01; OS06T0699400-01; OS06G0699400.
GeneIDi4341956.
KEGGiosa:4341956.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF216316 mRNA. Translation: AAG40580.1 .
AJ512642 mRNA. Translation: CAD54741.1 .
AJ251330 mRNA. Translation: CAB61889.1 .
AP004278 Genomic DNA. Translation: BAD53997.1 .
RefSeqi NP_001058471.1. NM_001065006.1.
UniGenei Os.4602.

3D structure databases

ProteinModelPortali Q5Z859.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS06T0699400-01 ; OS06T0699400-01 ; OS06G0699400 .
GeneIDi 4341956.
KEGGi osa:4341956.

Organism-specific databases

Gramenei Q5Z859.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi Q5Z859.
KOi K04371.
OMAi MYPQAHP.

Enzyme and pathway databases

Reactomei REACT_203586. ERK/MAPK targets.
REACT_215626. ERK2 activation.
REACT_221522. ERKs are inactivated.
REACT_221537. ERK1 activation.
REACT_224658. Regulation of HSF1-mediated heat shock response.
REACT_233484. Signal transduction by L1.
REACT_248492. ISG15 antiviral mechanism.
REACT_261734. Signaling by FGFR.
REACT_268490. Signalling to ERK5.

Gene expression databases

ExpressionAtlasi Q5Z859. baseline.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
    Wen J.-Q., Oono K., Imai R.
    Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
    Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
    Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  3. "Molecular cloning and expression of a MAP kinase homologue from rice."
    Huang H.-J., Fu S.-F., Tai Y.-H., Huang D.-D., Kuo T.T.
    Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK4_ORYSJ
AccessioniPrimary (citable) accession number: Q5Z859
Secondary accession number(s): Q8H0P1, Q9FQM2, Q9SMA9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 23, 2004
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3