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Q5Z859 (MPK4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 4

Short name=MAP kinase 4
EC=2.7.11.24
Alternative name(s):
Multiple stress-responsive MAP kinase 3
OsMAP2
OsMSRMK3
Gene names
Name:MPK4
Synonyms:MAP2, MSRMK3
Ordered Locus Names:Os06g0699400, LOC_Os06g48590
ORF Names:P0468G03.11
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Tissue specificity

Expressed in leaves and panicles. Ref.2

Induction

By stresses, hormones, heavy metals, phosphatase inhibitors and infection with rice blast fungus (M.grisea). Down-regulated by drought, high/low temperature and UV-C. Ref.2 Ref.5

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Biological processStress response
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to hydrogen peroxide

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

MAP kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Mitogen-activated protein kinase 4
PRO_0000239747

Regions

Domain32 – 319288Protein kinase
Nucleotide binding38 – 469ATP By similarity
Motif191 – 1933TXY

Sites

Active site1581Proton acceptor By similarity
Binding site611ATP By similarity

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity

Experimental info

Sequence conflict21A → V Ref.2
Sequence conflict21A → V Ref.3
Sequence conflict321Y → V in CAB61889. Ref.3
Sequence conflict3311A → V in CAB61889. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q5Z859 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: D21EBE45865CE08B

FASTA36942,244
        10         20         30         40         50         60 
MAMMVDPPNG MGNQGKHYYT MWQTLFEIDT KYVPIKPIGR GAYGIVCSSI NRATNEKVAI 

        70         80         90        100        110        120 
KKINNVFDNR VDALRTLREL KLLRHLRHEN VIALKDIMMP VHRRSFKDVY LVYELMDTDL 

       130        140        150        160        170        180 
HQIIKSSQPL SNDHCQYFLF QLLRGLKYLH SAGILHRDLK PGNLLVNANC DLKICDFGLA 

       190        200        210        220        230        240 
RTNNTKGQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFAELLGR KPIFPGTECL 

       250        260        270        280        290        300 
NQLKLIVNVL GTMSEADIEF IDNPKARKYI KTLPYTPGIP LTSMYPQAHP LAIDLLQKML 

       310        320        330        340        350        360 
VFDPSKRISV TEALEHPYMS PLYDPSANPP AQVPIDLDID ENLGVDMIRE MMWQEMLHYH 


PEVVAGVNM 

« Hide

References

« Hide 'large scale' references
[1]"Two novel mitogen-activated protein signaling components, OsMEK1 and OsMAP1, are involved in a moderate low-temperature signaling pathway in rice."
Wen J.-Q., Oono K., Imai R.
Plant Physiol. 129:1880-1891(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Nipponbare.
Tissue: Leaf.
[3]"Molecular cloning and expression of a MAP kinase homologue from rice."
Huang H.-J., Fu S.-F., Tai Y.-H., Huang D.-D., Kuo T.T.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF216316 mRNA. Translation: AAG40580.1.
AJ512642 mRNA. Translation: CAD54741.1.
AJ251330 mRNA. Translation: CAB61889.1.
AP004278 Genomic DNA. Translation: BAD53997.1.
RefSeqNP_001058471.1. NM_001065006.1.
UniGeneOs.4602.

3D structure databases

ProteinModelPortalQ5Z859.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0699400-01; OS06T0699400-01; OS06G0699400.
GeneID4341956.
KEGGosa:4341956.

Organism-specific databases

GrameneQ5Z859.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK04371.
OMAMYPQAHP.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK4_ORYSJ
AccessionPrimary (citable) accession number: Q5Z859
Secondary accession number(s): Q8H0P1, Q9FQM2, Q9SMA9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations