Q5Z6B1 (SEN2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA-splicing endonuclease subunit Sen2
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease Sen2

Gene names

Ordered Locus Names:	Os06g0530700, LOC_Os06g33980
ORF Names:	P0410C01.17, P0438E12.38

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron.
Subunit structure	tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.
Subcellular location	Nucleus By similarity. Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the tRNA-intron endonuclease family.

Ontologies

Keywords
Biological process	mRNA processing tRNA processing
Cellular component	Membrane Nucleus
Domain	Transmembrane Transmembrane helix
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 293	293	Probable tRNA-splicing endonuclease subunit Sen2		PRO_0000109458
Regions
	Transmembrane	267 – 287	21	Helical; Potential
	Compositional bias	170 – 173	4	Poly-Val
	Compositional bias	212 – 215	4	Poly-Ser
Sites
	Active site	157	1	By similarity
	Active site	165	1	By similarity
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z6B1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 673DE4101F5408C1	FASTA	293	32,737
	10 20 30 40 50 60 MDLPGPRWKK GKDGKDFASL AAANPMSAIV SELKASFISS KPVAILSGPG GSAVLGVGPE 70 80 90 100 110 120 QAVILNRAAF GHAIENATAQ KHWFQLSPEE VFYLCHALNC IRVDSLDNKQ MSEIELWDYF 130 140 150 160 170 180 RSGSESFPEM YKAYAHLRLK NWVVRSGLQY GADFVAYRHH PALVHSEFAV VVVPEGAEFG 190 200 210 220 230 240 NRCGRLEVWS DLLCALRASG SVAKTLLVLT ISSSSKCELS SPDCLEQLVV HERTITRWIL 250 260 270 280 290 QQCREQRCEP SRDEVNREEL IIEKESVVFN HWGVILGFTV LSGLLVYRLK FRQ

References

[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP004749 Genomic DNA. Translation: BAD54220.1. AP005450 Genomic DNA. Translation: BAD54480.1. AK059057 mRNA. No translation available. AK102915 mRNA. No translation available.
RefSeq	NP_001057781.1. NM_001064316.1.
UniGene	Os.24713.
3D structure databases
ProteinModelPortal	Q5Z6B1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os06g33980.1; LOC_Os06g33980.1; LOC_Os06g33980. LOC_Os06g33980.2; LOC_Os06g33980.2; LOC_Os06g33980.
GeneID	4341203.
KEGG	osa:4341203.
Organism-specific databases
Gramene	Q5Z6B1.
Phylogenomic databases
eggNOG	COG1676.
KO	K15322.
OMA	CLENEVD.
ProtClustDB	CLSN2684758.
Gene expression databases
ArrayExpress	Q5Z6B1.
Family and domain databases
Gene3D	3.40.1350.10. 1 hit.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 1 hit. PF02778. tRNA_int_endo_N. 1 hit. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SEN2_ORYSJ

Accession

Primary (citable) accession number: Q5Z6B1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	November 23, 2004
Last modified:	April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program