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Q5Z6B1 (SEN2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable tRNA-splicing endonuclease subunit Sen2

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease Sen2
Gene names
Ordered Locus Names:Os06g0530700, LOC_Os06g33980
ORF Names:P0410C01.17, P0438E12.38
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Probably carries the active site for 5'-splice site cleavage By similarity.

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.

Subunit structure

tRNA splicing endonuclease is a heterotetramer composed of SEN2, SEN15, SEN34/LENG5 and SEN54 By similarity.

Subcellular location

Nucleus By similarity. Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the tRNA-intron endonuclease family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293Probable tRNA-splicing endonuclease subunit Sen2
PRO_0000109458

Regions

Transmembrane267 – 28721Helical; Potential
Compositional bias170 – 1734Poly-Val
Compositional bias212 – 2154Poly-Ser

Sites

Active site1571 By similarity
Active site1651 By similarity
Active site2041 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z6B1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 673DE4101F5408C1

FASTA29332,737
        10         20         30         40         50         60 
MDLPGPRWKK GKDGKDFASL AAANPMSAIV SELKASFISS KPVAILSGPG GSAVLGVGPE 

        70         80         90        100        110        120 
QAVILNRAAF GHAIENATAQ KHWFQLSPEE VFYLCHALNC IRVDSLDNKQ MSEIELWDYF 

       130        140        150        160        170        180 
RSGSESFPEM YKAYAHLRLK NWVVRSGLQY GADFVAYRHH PALVHSEFAV VVVPEGAEFG 

       190        200        210        220        230        240 
NRCGRLEVWS DLLCALRASG SVAKTLLVLT ISSSSKCELS SPDCLEQLVV HERTITRWIL 

       250        260        270        280        290 
QQCREQRCEP SRDEVNREEL IIEKESVVFN HWGVILGFTV LSGLLVYRLK FRQ 

« Hide

References

[1]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP004749 Genomic DNA. Translation: BAD54220.1.
AP005450 Genomic DNA. Translation: BAD54480.1.
AK059057 mRNA. No translation available.
AK102915 mRNA. No translation available.
RefSeqNP_001057781.1. NM_001064316.1.
UniGeneOs.24713.

3D structure databases

ProteinModelPortalQ5Z6B1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING39947.LOC_Os06g33980.2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS06T0530700-02; OS06T0530700-02; OS06G0530700.
GeneID4341203.
KEGGosa:4341203.

Organism-specific databases

GrameneQ5Z6B1.

Phylogenomic databases

eggNOGCOG1676.
KOK15322.
OMATITRWSP.

Family and domain databases

Gene3D3.40.1350.10. 1 hit.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
[Graphical view]
PfamPF01974. tRNA_int_endo. 1 hit.
PF02778. tRNA_int_endo_N. 1 hit.
[Graphical view]
SUPFAMSSF53032. SSF53032. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSEN2_ORYSJ
AccessionPrimary (citable) accession number: Q5Z6B1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations