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Q5Z3Z3 (Q5Z3Z3_NOCFA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Protein attributes

Sequence length696 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. RuleBase RU003363 HAMAP-Rule MF_01898

Catalytic activity

ATP-dependent breakage, passage and rejoining of double-stranded DNA. RuleBase RU003363 HAMAP-Rule MF_01898 SAAS SAAS013506

Cofactor

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity. HAMAP-Rule MF_01898

Subunit structure

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01898

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01898.

Sequence similarities

Belongs to the type II topoisomerase family. HAMAP-Rule MF_01898 RuleBase RU000380

Contains 1 Toprim domain. HAMAP-Rule MF_01898

Contains Toprim domain. SAAS SAAS011557

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain474 – 588115Toprim By similarity HAMAP-Rule MF_01898

Sites

Metal binding4801Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5531Magnesium 1; catalytic By similarity HAMAP-Rule MF_01898
Metal binding5531Magnesium 2 By similarity HAMAP-Rule MF_01898
Metal binding5551Magnesium 2 By similarity HAMAP-Rule MF_01898
Site5051Interaction with DNA By similarity HAMAP-Rule MF_01898
Site5081Interaction with DNA By similarity HAMAP-Rule MF_01898

Sequences

Sequence LengthMass (Da)Tools
Q5Z3Z3 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 26B9306DC1221E59

FASTA69676,678
        10         20         30         40         50         60 
MCCAIATRRS ASKESYRPVA AKHSNESGSK STNYGASSIT VLEGLEAVRK RPGMYIGSTG 

        70         80         90        100        110        120 
ERGLHHLIWE VVDNSVDEAM AGYATRVDVT LLADGGVEVV DDGRGIPVEM HAQGIPTVEV 

       130        140        150        160        170        180 
VMTQLHAGGK FDSESYAVSG GLHGVGISVV NALSSRVEVE IDVDGYHWSQ TYKEATPSKL 

       190        200        210        220        230        240 
VKGEPTDRTG TTVRFWADPE IFETTTYNFE TVARRLQEMA FLNKGLTITL TDERVSESDV 

       250        260        270        280        290        300 
TDEIVSETAE APKHGEPTGE AASEHKVKTR TYHYPGGLED FVRHINRTKQ AIHNSVVSFT 

       310        320        330        340        350        360 
GKGTGHELEV AMQWNSGYSE SVHTFANTIN THEGGTHEEG FRAALTTVVN KYAKDKKLLK 

       370        380        390        400        410        420 
EKDGNLTGDD IREGLAAIVS VKVAEPQFEG QTKTKLGNTE VKSFVQRTCN EHLTHWFEAN 

       430        440        450        460        470        480 
PADAKTIVQK AVSSAQARVA ARKARELVRR KSATDLGGLP GKLADCRSKD PSKSEIYIVE 

       490        500        510        520        530        540 
GDSAGGSAKS GRDSMYQAIL PLRGKIINVE KARIDKVLKN NEVQSIITAF GTGIHDEFDI 

       550        560        570        580        590        600 
SKLRYHKIVL MADADVDGQH ISTLLLTLLF RFMRPLVEHG HVYLAQPPLY KLKWQRSEPE 

       610        620        630        640        650        660 
FAYSDRERDG LLERGLAAGK KINKDDGIQR YKGLGEMNPK ELWETTMDPS VRVLRQVTLD 

       670        680        690 
DAAAADELFS VLMGEDVEAR RSFITRNAKD VRFLDV 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152 EMBL BAD54848.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD54848.1.
RefSeqYP_116212.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5Z3Z3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa60.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD54848; BAD54848; NFA_60.
GeneID3109626.
KEGGnfa:nfa60.
PATRIC22729460. VBINocFar94200_0006.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK02470.
OMACCAIATR.
OrthoDBEOG6P334W.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-6-MONOMER.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPMF_01898. GyrB.
InterProIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00418. TPI2FAMILY.
SMARTSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsTIGR01059. gyrB. 1 hit.
PROSITEPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ5Z3Z3_NOCFA
AccessionPrimary (citable) accession number: Q5Z3Z3
Entry history
Integrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)