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Q5Z3Z3

- Q5Z3Z3_NOCFA

UniProt

Q5Z3Z3 - Q5Z3Z3_NOCFA

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Protein
DNA gyrase subunit B
Gene
gyrB, NFA_60
Organism
Nocardia farcinica (strain IFM 10152)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity.UniRule annotation

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotationSAAS annotations

Cofactori

Magnesium. Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ and Ca2+ By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi480 – 4801Magnesium 1; catalytic By similarityUniRule annotation
Sitei505 – 5051Interaction with DNA By similarityUniRule annotation
Sitei508 – 5081Interaction with DNA By similarityUniRule annotation
Metal bindingi553 – 5531Magnesium 1; catalytic By similarityUniRule annotation
Metal bindingi553 – 5531Magnesium 2 By similarityUniRule annotation
Metal bindingi555 – 5551Magnesium 2 By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. DNA binding Source: UniProtKB-HAMAP
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: InterPro
  2. DNA-dependent DNA replication Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, DNA-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-6-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA gyrase subunit BUniRule annotation (EC:5.99.1.3UniRule annotation)
Gene namesi
Name:gyrBUniRule annotationImported
Ordered Locus Names:NFA_60Imported
OrganismiNocardia farcinica (strain IFM 10152)Imported
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa60.

Structurei

3D structure databases

ProteinModelPortaliQ5Z3Z3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini474 – 588115Toprim By similarityUniRule annotation
Add
BLAST

Sequence similaritiesi

Contains 1 Toprim domain.UniRule annotation
Contains Toprim domain.SAAS annotations

Phylogenomic databases

KOiK02470.
OMAiCCAIATR.
OrthoDBiEOG6P334W.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPiMF_01898. GyrB.
InterProiIPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view]
PfamiPF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00418. TPI2FAMILY.
SMARTiSM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsiTIGR01059. gyrB. 1 hit.
PROSITEiPS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5Z3Z3-1 [UniParc]FASTAAdd to Basket

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MCCAIATRRS ASKESYRPVA AKHSNESGSK STNYGASSIT VLEGLEAVRK    50
RPGMYIGSTG ERGLHHLIWE VVDNSVDEAM AGYATRVDVT LLADGGVEVV 100
DDGRGIPVEM HAQGIPTVEV VMTQLHAGGK FDSESYAVSG GLHGVGISVV 150
NALSSRVEVE IDVDGYHWSQ TYKEATPSKL VKGEPTDRTG TTVRFWADPE 200
IFETTTYNFE TVARRLQEMA FLNKGLTITL TDERVSESDV TDEIVSETAE 250
APKHGEPTGE AASEHKVKTR TYHYPGGLED FVRHINRTKQ AIHNSVVSFT 300
GKGTGHELEV AMQWNSGYSE SVHTFANTIN THEGGTHEEG FRAALTTVVN 350
KYAKDKKLLK EKDGNLTGDD IREGLAAIVS VKVAEPQFEG QTKTKLGNTE 400
VKSFVQRTCN EHLTHWFEAN PADAKTIVQK AVSSAQARVA ARKARELVRR 450
KSATDLGGLP GKLADCRSKD PSKSEIYIVE GDSAGGSAKS GRDSMYQAIL 500
PLRGKIINVE KARIDKVLKN NEVQSIITAF GTGIHDEFDI SKLRYHKIVL 550
MADADVDGQH ISTLLLTLLF RFMRPLVEHG HVYLAQPPLY KLKWQRSEPE 600
FAYSDRERDG LLERGLAAGK KINKDDGIQR YKGLGEMNPK ELWETTMDPS 650
VRVLRQVTLD DAAAADELFS VLMGEDVEAR RSFITRNAKD VRFLDV 696
Length:696
Mass (Da):76,678
Last modified:November 23, 2004 - v1
Checksum:i26B9306DC1221E59
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD54848.1.
RefSeqiYP_116212.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD54848; BAD54848; NFA_60.
GeneIDi3109626.
KEGGinfa:nfa60.
PATRICi22729460. VBINocFar94200_0006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD54848.1 .
RefSeqi YP_116212.1. NC_006361.1.

3D structure databases

ProteinModelPortali Q5Z3Z3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 247156.nfa60.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD54848 ; BAD54848 ; NFA_60 .
GeneIDi 3109626.
KEGGi nfa:nfa60.
PATRICi 22729460. VBINocFar94200_0006.

Phylogenomic databases

KOi K02470.
OMAi CCAIATR.
OrthoDBi EOG6P334W.

Enzyme and pathway databases

BioCyci NFAR247156:GJ9T-6-MONOMER.

Family and domain databases

Gene3Di 3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
3.40.50.670. 1 hit.
HAMAPi MF_01898. GyrB.
InterProi IPR002288. DNA_gyrase_B_C.
IPR011557. GyrB.
IPR003594. HATPase_ATP-bd.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR001241. Topo_IIA.
IPR013506. Topo_IIA_bsu_dom2.
IPR013759. Topo_IIA_cen_dom.
IPR013760. Topo_IIA_like_dom.
IPR018522. TopoIIA_CS.
IPR006171. Toprim_domain.
[Graphical view ]
Pfami PF00204. DNA_gyraseB. 1 hit.
PF00986. DNA_gyraseB_C. 1 hit.
PF02518. HATPase_c. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00418. TPI2FAMILY.
SMARTi SM00387. HATPase_c. 1 hit.
SM00433. TOP2c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF56719. SSF56719. 1 hit.
TIGRFAMsi TIGR01059. gyrB. 1 hit.
PROSITEi PS00177. TOPOISOMERASE_II. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152Imported.

Entry informationi

Entry nameiQ5Z3Z3_NOCFA
AccessioniPrimary (citable) accession number: Q5Z3Z3
Entry historyi
Integrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

Similar proteinsi