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Q5Z380 (GLUQ_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamyl-Q tRNA(Asp) synthetase
Short name=Glu-Q-RSs
EC=6.1.1.-
Gene names
Name:gluQ
Ordered Locus Names:NFA_2690
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity. HAMAP MF_01428

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01428

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428
PRO_0000208309

Regions

Region14 – 185Glutamate binding By similarity
Motif17 – 2711"HIGH" region HAMAP MF_01428
Motif242 – 2465"KMSKS" region HAMAP MF_01428

Sites

Metal binding1041Zinc By similarity
Metal binding1061Zinc By similarity
Metal binding1251Zinc By similarity
Metal binding1291Zinc By similarity
Binding site501Glutamate By similarity
Binding site1861Glutamate By similarity
Binding site2041Glutamate By similarity
Binding site2451ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z380 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 309C2A1C243AE179

FASTA31134,076
        10         20         30         40         50         60 
MADVQAPTIG GAGRYAPSPS GDLHLGNLRT ALLAWLFARA SGRRFLLRVE DLDRVRPGAQ 

        70         80         90        100        110        120 
QRQLADLRAI GLDWDGPVVC QSQRRTRYEA AITRLREAGL TYECYCTRRE IQQAATAPHG 

       130        140        150        160        170        180 
PLGAYPGTCR DLSDADRAER RAQGRPAALR LRAERTDFEI VDQLHGRYRG PVDDVVLRRG 

       190        200        210        220        230        240 
DGLPAYNLAV VVDDAAQGVD QVVRGDDLLP STPRQAYLAT LLGLPVPAYA HVPLVLNKDG 

       250        260        270        280        290        300 
QRLAKRDGAV TLADQQRAGK SPEQVLALLT DSLGLPPRPP RELLADFDPA RLPREPWILT 

       310 
DNGLLQPSEC P

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006618 Genomic DNA. Translation: BAD55111.1.
RefSeqYP_116475.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5Z380.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3106998.
GenomeReviewsGene locus NFA_2690 in contig AP006618_GR.
KEGGnfa:nfa2690.
NMPDRfig|247156.1.peg.339.
PATRIC22729998. VBINocFar94200_0267.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG628189.
OMADAPSSYH.
ProtClustDBPRK05710.

Enzyme and pathway databases

BioCycNFAR247156:NFA2690-MONOMER.

Family and domain databases

HAMAPMF_01428. Glu_Q_tRNA_synth.
[Tree]
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR022380. Glu-Q_TRNA(Asp)_Synthase.
IPR000924. Glu/Gln-tRNA-synth_Ib.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01885.
PANTHERPTHR10119. Glu_tRNA-synt_1c. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
TIGRFAMsTIGR03838. Queuosine_YadB. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLUQ_NOCFA
AccessionPrimary (citable) accession number: Q5Z380
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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