Reviewed, UniProtKB/Swiss-Prot Q5Z380 (GLUQ_NOCFA)

Names and origin

Protein names

Recommended name:
    Glutamyl-Q tRNA(Asp) synthetase
      Short name=Glu-Q-RSs
    EC=6.1.1.-

Gene names

Name:	gluQ
Ordered Locus Names:	NFA_2690

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble position of the QUC anticodon By similarity. HAMAP MF_01428
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01428
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily.

Ontologies

Keywords
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Glutamyl-Q tRNA(Asp) synthetase HAMAP MF_01428		PRO_0000208309
Regions
	Region	14 – 18	5	Glutamate binding By similarity
	Motif	17 – 27	11	"HIGH" region HAMAP MF_01428
	Motif	242 – 246	5	"KMSKS" region HAMAP MF_01428
Sites
	Metal binding	104	1	Zinc By similarity
	Metal binding	106	1	Zinc By similarity
	Metal binding	125	1	Zinc By similarity
	Metal binding	129	1	Zinc By similarity
	Binding site	50	1	Glutamate By similarity
	Binding site	186	1	Glutamate By similarity
	Binding site	204	1	Glutamate By similarity
	Binding site	245	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z380-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 309C2A1C243AE179	FASTA	311	34,076
	10 20 30 40 50 60 MADVQAPTIG GAGRYAPSPS GDLHLGNLRT ALLAWLFARA SGRRFLLRVE DLDRVRPGAQ 70 80 90 100 110 120 QRQLADLRAI GLDWDGPVVC QSQRRTRYEA AITRLREAGL TYECYCTRRE IQQAATAPHG 130 140 150 160 170 180 PLGAYPGTCR DLSDADRAER RAQGRPAALR LRAERTDFEI VDQLHGRYRG PVDDVVLRRG 190 200 210 220 230 240 DGLPAYNLAV VVDDAAQGVD QVVRGDDLLP STPRQAYLAT LLGLPVPAYA HVPLVLNKDG 250 260 270 280 290 300 QRLAKRDGAV TLADQQRAGK SPEQVLALLT DSLGLPPRPP RELLADFDPA RLPREPWILT 310 DNGLLQPSEC P

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55111.1.
RefSeq	YP_116475.1.
3D structure databases
SMR	Q5Z380. Positions 12-298.
ModBase	Search...
Genome annotation databases
GeneID	3106998.
GenomeReviews	Gene locus NFA_2690 in contig AP006618_GR.
KEGG	nfa:nfa2690.
NMPDR	fig|247156.1.peg.339.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DAPSSYH.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA2690-MONOMER.
Family and domain databases
HAMAP	MF_01428. Glu_Q_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLUQ_NOCFA

Accession

Primary (citable) accession number: Q5Z380

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	November 23, 2004
Last modified:	January 19, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)