Q5Z2Y2 (Q5Z2Y2_NOCFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL BAD55209.1
Ordered Locus Names:	NFA_3670

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	976 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5Z2Y2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 4917627D9F9D743D	FASTA	976	106,195
	10 20 30 40 50 60 MYSPVATRDR GAADQGRPLR RLVIVESPTK ARKIAPYLGR NYTVEASVGH IRDLPRGAAD 70 80 90 100 110 120 VPAKYKGQPW ARLGVDVDND FEPIYVVSPD KKAKVTELKS LLADADELYL ATDPDREGEA 130 140 150 160 170 180 IAWHLLETLK PKVPVRRMVF HEITEPAIRA AAADTRELDP DLVDAQETRR ILDRLYGYEV 190 200 210 220 230 240 SPVLWKKVMP KLSAGRVQSV ATRVIVQRER ERMAFRSAEY WDIAAKLDAG ADEQSDAANP 250 260 270 280 290 300 RTFGARLVNV DGARVASGRD FGPDGQLKSA SGVVVLDEAR ARRLAEALDG VDLAVTSAES 310 320 330 340 350 360 KPYSRKPYPP FMTSTLQQEA GRKLRFTSER TMRVAQRLYE NGYITYMRTD STTLSESAIA 370 380 390 400 410 420 AARSQATQLY GAEYVHPSPR QYNRKVKNAQ EAHEAIRPAG DTFATPGQLH SRLDNDEFRL 430 440 450 460 470 480 YELIWQRTVA SQMADARGTT LTLRITGVAG TGEECVFSAS GRTITFPGFL KAYVESVDEE 490 500 510 520 530 540 AGGQSDDAES RLPALEEGQN VTAVELNPDG HSTNPPARYT EASLIKALEE LGIGRPSTYS 550 560 570 580 590 600 SIIKTILDRG YVYKRGSALV PSWVAFAVVG LLEAYFGRLV DFDFTAAMED DLDAIASGRE 610 620 630 640 650 660 RRGNWLSSFY FGGDSGVEGS VARSGGLKKM VGEQLDDIDA REVNSIKLFT DAEGRDVVVR 670 680 690 700 710 720 VGRYGPYLER LVTDPDDPDG DPISQRANLP DDLPPDELTP EVAEKLFATP QEGRSLGVDP 730 740 750 760 770 780 ATGHEIVAKE GRFGPYVTEI LPEPATEEGA KKTAAKKTAP KPRTGSLFKS MDLASITLDD 790 800 810 820 830 840 ALKLLSLPRV VGTDPATGEE ITAQNGRYGP YLKKGTDSRS LASEDQIFTI TLDEALKIYA 850 860 870 880 890 900 EPKRRGGQSA SAAPLRELGT DPVSGKPMVI KDGRFGPYVT DGETNASLRK GDEVESITDE 910 920 930 940 950 960 RAAELLADRR ARGPVKKTAK KAAKKTAAKK TTATKTAATK TAATKTTAKK TTAKKTTAAK 970 KTAAKKAPAK STAENS

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55209.1.
RefSeq	YP_116573.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z2Y2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111603.
GenomeReviews	Gene locus NFA_3670 in contig AP006618_GR.
KEGG	nfa:nfa3670.
NMPDR	fig|247156.1.peg.434.
PATRIC	22730208. VBINocFar94200_0370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q5Z2Y2_NOCFA

Accession

Primary (citable) accession number: Q5Z2Y2

Entry history

Integrated into UniProtKB/TrEMBL:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)