Q5Z2R1 (SYC_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Synonyms:	cysS1
Ordered Locus Names:	NFA_4380

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159446
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	263 – 267	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	207	1	Zinc By similarity
	Metal binding	232	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Binding site	266	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z2R1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: F9213319BE0349E9	FASTA	467	52,087
	10 20 30 40 50 60 MTLRLFDTES RTMREFAPLV PGRASVYLCG ATVQGEPHIG HVRSGVAFDV LRRWLQAHDY 70 80 90 100 110 120 DVWFIRNVTD IDDKILNKAA EAGRPWWEWA ATYERAFDRA YRLLGVQPPS AEPRATGHIT 130 140 150 160 170 180 QMVELMRRLI ERGHAYASAG NVYFDVQSYP EYGALSGHKL DDVHQGESAG EGKRDPRDFT 190 200 210 220 230 240 LWKAAKPGEP SWPSPWGPGR PGWHLECSAM AEFYLGAEFD IHCGGMDLVF PHHENEIAQS 250 260 270 280 290 300 KAAGDGFARY WLHNGWVTMG GEKMSKSLGN VLSVPNMLTK VRAVELRFYL GSAHYRSMLE 310 320 330 340 350 360 YSDKALDDAV AGYQRIEAFL HRTAERVGEI PVGKWTDAFA EAMDDDLAVP RALAEVFRLV 370 380 390 400 410 420 TEGNKALEAG AVDTARELGG QVRAMLGILG VCPFDPQWDH KQDDSVAQTA LDVLVRAELD 430 440 450 460 RRQQARADKD WATADAVRDR LHAAGIDVTD TPNGPEWSLR TARGKAN

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55280.1.
RefSeq	YP_116644.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z2R1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109801.
GenomeReviews	Gene locus NFA_4380 in contig AP006618_GR.
KEGG	nfa:nfa4380.
NMPDR	fig|247156.1.peg.502.
PATRIC	22730358. VBINocFar94200_0444.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA4380-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_NOCFA

Accession

Primary (citable) accession number: Q5Z2R1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program