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Q5Z2R1 (SYC_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cysteine--tRNA ligase

EC=6.1.1.16
Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS
Gene names
Name:cysS
Synonyms:cysS1
Ordered Locus Names:NFA_4380
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00041

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00041

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00041.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcysteinyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cysteine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467Cysteine--tRNA ligase HAMAP-Rule MF_00041
PRO_0000159446

Regions

Motif31 – 4111"HIGH" region HAMAP-Rule MF_00041
Motif263 – 2675"KMSKS" region HAMAP-Rule MF_00041

Sites

Metal binding291Zinc By similarity
Metal binding2071Zinc By similarity
Metal binding2321Zinc By similarity
Metal binding2361Zinc By similarity
Binding site2661ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z2R1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: F9213319BE0349E9

FASTA46752,087
        10         20         30         40         50         60 
MTLRLFDTES RTMREFAPLV PGRASVYLCG ATVQGEPHIG HVRSGVAFDV LRRWLQAHDY 

        70         80         90        100        110        120 
DVWFIRNVTD IDDKILNKAA EAGRPWWEWA ATYERAFDRA YRLLGVQPPS AEPRATGHIT 

       130        140        150        160        170        180 
QMVELMRRLI ERGHAYASAG NVYFDVQSYP EYGALSGHKL DDVHQGESAG EGKRDPRDFT 

       190        200        210        220        230        240 
LWKAAKPGEP SWPSPWGPGR PGWHLECSAM AEFYLGAEFD IHCGGMDLVF PHHENEIAQS 

       250        260        270        280        290        300 
KAAGDGFARY WLHNGWVTMG GEKMSKSLGN VLSVPNMLTK VRAVELRFYL GSAHYRSMLE 

       310        320        330        340        350        360 
YSDKALDDAV AGYQRIEAFL HRTAERVGEI PVGKWTDAFA EAMDDDLAVP RALAEVFRLV 

       370        380        390        400        410        420 
TEGNKALEAG AVDTARELGG QVRAMLGILG VCPFDPQWDH KQDDSVAQTA LDVLVRAELD 

       430        440        450        460 
RRQQARADKD WATADAVRDR LHAAGIDVTD TPNGPEWSLR TARGKAN 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD55280.1.
RefSeqYP_116644.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5Z2R1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa4380.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD55280; BAD55280; NFA_4380.
GeneID3109801.
KEGGnfa:nfa4380.
PATRIC22730358. VBINocFar94200_0444.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0215.
KOK01883.
OMAQIDIHAG.
OrthoDBEOG6RVFXC.
ProtClustDBPRK00260.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-450-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00041. Cys_tRNA_synth.
InterProIPR015803. Cys-tRNA-ligase.
IPR015273. Cys-tRNA-synt_Ia_DALR.
IPR024909. Cys-tRNA/MSH_ligase.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERPTHR10890. PTHR10890. 1 hit.
PfamPF09190. DALR_2. 1 hit.
PF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SMARTSM00840. DALR_2. 1 hit.
[Graphical view]
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00435. cysS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYC_NOCFA
AccessionPrimary (citable) accession number: Q5Z2R1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 23, 2004
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries