Q5Z1E9 (GUAA_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	guaA
Ordered Locus Names:	NFA_8970

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00344
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00344
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00344
Subunit structure	Homodimer By similarity. HAMAP MF_00344
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	GMP synthase [glutamine-hydrolyzing] HAMAP MF_00344		PRO_0000229449
Regions
	Domain	7 – 196	190	Glutamine amidotransferase type-1
	Domain	197 – 398	202	GMPS ATP-PPase
	Nucleotide binding	224 – 230	7	ATP By similarity
Sites
	Active site	84	1	Nucleophile By similarity
	Active site	170	1	By similarity
	Active site	172	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z1E9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 37506C116CFF9C8C	FASTA	524	56,334
	10 20 30 40 50 60 MAETQRPVLV VDFGAQYAQL IARRVREASV YSEVVPHTAT VEEIAAKKPL AVILSGGPAS 70 80 90 100 110 120 VYAEGAPQLD PRLFDLNLPV FGICYGFQAM AQALGGTVAH TGTREYGRTE LNIDGGVLHG 130 140 150 160 170 180 GLPTIQPVWM SHGDAVTDAP EGFEVTGTTA GAPVAAFEDR ARRLAGVQYH PEVLHSPHGQ 190 200 210 220 230 240 QVLSRFLHEL AGIPASWTPS NIADVLVEQV RAQVGDGHAI CGLSGGVDSA VAAALVQRAI 250 260 270 280 290 300 GDRLTCVFVD HGLLRAGERE QVQQDFVAST GAKLVTVDAV DKFLGELKGV TDPEEKRKII 310 320 330 340 350 360 GREFIRSFED AVRQIVDEQG AAEGKQPEVE FLVQGTLYPD VVESGGGTGT ANIKSHHNVG 370 380 390 400 410 420 GLPEDLEFEL VEPLRLLFKD EVRAVGRELG LPEEIVARQP FPGPGLAIRI IGEVTADRLA 430 440 450 460 470 480 TLRQADAIAR EELTAAGLDR QIWQCPVVLL ADVRSVGVQG DGRTYGHPIV LRPVSSEDAM 490 500 510 520 TADWTRLPYE VLERISTRIT NEVAEVNRVV LDVTSKPPGT IEWE

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55742.1.
RefSeq	YP_117106.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z1E9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109622.
GenomeReviews	Gene locus NFA_8970 in contig AP006618_GR.
KEGG	nfa:nfa8970.
NMPDR	fig|247156.1.peg.940.
PATRIC	22731336. VBINocFar94200_0927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	DGRTYEY.
ProtClustDB	PRK00074.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA8970-MONOMER.
Family and domain databases
HAMAP	MF_00344. GMP_synthase. [Tree]
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_NOCFA

Accession

Primary (citable) accession number: Q5Z1E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program