Q5Z1D1 (DNAE2_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	NFA_9150

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	1146 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1146	1146	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103386

Sequences

	Sequence		Length	Mass (Da)
	Q5Z1D1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D02899A853403CEF	FASTA	1,146	123,632
	10 20 30 40 50 60 MGWGTGPPSW SELERVLSGR PGRTDPEAMY PGDGGDSPAW SRKREPYLAE PIRPVDRPTV 70 80 90 100 110 120 PYAELHAHSA YSFLDGASQP EELVEEAVRL GLEAIALTDH DGFYGTVRFA EAAKEWGIAT 130 140 150 160 170 180 VFGAELSLGV ARPAPRRSAR KAARGRGRAV RYAATPEFEH AAAPDSAPRT GEPDPPGPHL 190 200 210 220 230 240 LVLARGQEGY RRLSREIAAA HLAAGEKGVL RYDIDALTAA AGGHWHILTG CRKGHLRTAL 250 260 270 280 290 300 ADDLAAGETG LPRAEAALRD LVERFGADRV SVELTHHGVP ADDERNALLI ALADRVGLPV 310 320 330 340 350 360 LATTGAHFAG PEQRRRAMAL AAIRARRSLD EMAGWLAPAG GAHLRSGAEM ARLFAACPDA 370 380 390 400 410 420 VANAVALSRE CAFDLRLIAP QLPPFAVPDG HDENSWLREL VLRGAARRYG PPHANPPAYR 430 440 450 460 470 480 QLEHELEVIA TLGFPGYFLV VHDIVSFCER NGILCQGRGS AANSAVCFAI GITNVDPVAN 490 500 510 520 530 540 ELLFERFLSP ERDGPPDIDI DIESDRREEA IQHVYARYGR EYAAQVANVI TYRGKSAVRD 550 560 570 580 590 600 AAKALGFSPG QQDAWSKQVS RWTGVGAETG TDIPEQVLRL AADLEGLPRH MGIHSGGMVI 610 620 630 640 650 660 CDRPIADVCP VEWARMAGRS VLQWDKDDCA AVGLVKFDML GLGMLSALHY MIDLVREHEG 670 680 690 700 710 720 VEVRLHELDL KEPAVYEMLS RADSVGVFQV ESRAQMATLP RLRPREFYDL VVEVALIRPG 730 740 750 760 770 780 PIQGGSVHPY IRRRNGTEPV TYDHPALENS LGRTLGVPLF QEQLMQMAVD VAGFTAAEAD 790 800 810 820 830 840 QLRRAMGSKR SPERMRRLKE RLYRGMAELH GITGEVADRI YEKLYAFANF GFPESHSQSF 850 860 870 880 890 900 AALVFYSAWF KLHHPAAFCA GLLRAQPMGF YSPQSLVADA RRHGVPVHGP DVNASLAEAT 910 920 930 940 950 960 LENGGREVRL GLAGVRHIGS ELAERIVAAR VEHGPYTSVL DLTGRVELTV AQAEALATAG 970 980 990 1000 1010 1020 AFDSVTGPSG AERSGGRRAA LWAAGAAARE RAHLLPGTGP AADAPALPGM SALELAAADV 1030 1040 1050 1060 1070 1080 WATGISPGSY PTEFLRARMD ELGVIPAGRL LEVRDGSRVL VGGAVTHRQR PATAAGVTFL 1090 1100 1110 1120 1130 1140 NLEDETGMVN VVCSVGLWTR YRALAQSASA LLVRGRVQNA EGAVSVVAEH LQLLSLGMGS RSRDFR

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55760.1.
RefSeq	YP_117124.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z1D1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109823.
GenomeReviews	Gene locus NFA_9150 in contig AP006618_GR.
KEGG	nfa:nfa9150.
NMPDR	fig|247156.1.peg.955.
PATRIC	22731368. VBINocFar94200_0943.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	MFQIESP.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA9150-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_NOCFA

Accession

Primary (citable) accession number: Q5Z1D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program