Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5Z0W8 (GLGB_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:NFA_10780
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7447441,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188721

Sites

Active site4241Nucleophile By similarity
Active site4761Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z0W8 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: EAF4657E36A7DAD4

FASTA74482,575
        10         20         30         40         50         60 
MSGPEDPADR RHGEVPAPRR DIPRPLVERR DLMLLAAGTH RDPHTVLGAH PHPDGTVVRV 

        70         80         90        100        110        120 
LRPHAAKVSV RVGGVDHPLD SVGYGVFAAV LPHPELMDYR IVTEYPGGPT VIAADGFRFL 

       130        140        150        160        170        180 
PTLGELDLHL IGEGRHEHLW HVLGAHPRRY TTLDGPVSGT SFAVWAPNAR GVTVIGDFDG 

       190        200        210        220        230        240 
WSGNTAPMRA LGTSGVWEVF VPDVGSGTKY KYRVHGADGR VVDHADPLAF ATELPPATAS 

       250        260        270        280        290        300 
VVTGSDYTWQ DAQWLERRRS TDPTRSPMSV YEVHLGSWRP GLGYRELAEQ LADYVRAAGY 

       310        320        330        340        350        360 
THVELLPVAE HPFGGSWGYQ VTSYYAPTAR FGSPDDFRAF VDHLHGAGIG VLLDWVPAHF 

       370        380        390        400        410        420 
PRDEWALARF DGTPLYEHPD PRRGEQLDWG TYVFDFGRNE VRNFLVANAR FWIEEFHIDG 

       430        440        450        460        470        480 
LRVDAVASML YLDYSRGENW EPNIHGGREN LEAVAFLREL NDVVHRAHPG VVTIAEESTT 

       490        500        510        520        530        540 
WPGVTRGTEV GGLGFTMKWN MGWMHDTLGF LARDPIHRSW HHNEITFSLV YAWSENYVLP 

       550        560        570        580        590        600 
ISHDEVVHGK GTLWTRMPGD DFAKAAGVRA LLAYMWAHPG KQLLFMGQDF GQFREWSHDR 

       610        620        630        640        650        660 
GLDWGELDNP LHRGIATAVR DLNRVYRATP ALWSQDTTPG GYAWIEADDR DRNVLAFLRY 

       670        680        690        700        710        720 
GSDGSTVACV FNFSGALHGE YRVGLPIAGE WTEILNTDAA DYGGSGVGNY GVVRTEEIPW 

       730        740 
HGRPVSATVA LAPNSAVWLA APRA 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD55923.1.
RefSeqYP_117287.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5Z0W8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa10780.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD55923; BAD55923; NFA_10780.
GeneID3109649.
KEGGnfa:nfa10780.
PATRIC22731722. VBINocFar94200_1114.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
KOK00700.
OMAVACVFNF.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-1106-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_NOCFA
AccessionPrimary (citable) accession number: Q5Z0W8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: November 23, 2004
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries