Q5Z0V0 (NTPA_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

NFA_10960

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178202
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	75 – 76	2	Substrate binding By similarity
Sites
	Metal binding	75	1	Magnesium or manganese By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	181	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z0V0 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 8AAED92038D652B3	FASTA	206	21,245
	10 20 30 40 50 60 MTMARVLVAS RNAKKLAELR RILDDAGVAG VQIVGLDDVP PYDEAPETGA TFEENALAKA 70 80 90 100 110 120 RDGAAATGLP CVADDSGLAV DALNGMPGVL SARWSGTHGD DAANNALLLA QLRDVPDERR 130 140 150 160 170 180 GARFVSACAL VVPGGTETVV RGEWPGTIGR KPMGEGGFGY DPLFVPDGGD VTAAQLTPAA 190 200 KDAASHRGRA LRHLLPALAA LADRTE

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD55941.1.
RefSeq	YP_117305.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z0V0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3106565.
GenomeReviews	Gene locus NFA_10960 in contig AP006618_GR.
KEGG	nfa:nfa10960.
NMPDR	fig|247156.1.peg.1129.
PATRIC	22731760. VBINocFar94200_1133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA10960-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_NOCFA

Accession

Primary (citable) accession number: Q5Z0V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program