Q5Z0M4 (CLPP1_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 1
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	NFA_11720

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.
Caution	Ala-101 is present instead of the conserved Ser which is expected to be an active site residue.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179604
Sites
	Active site	126	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z0M4 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 2399620DBC956EF8	FASTA	197	21,275
	10 20 30 40 50 60 MSTYTIPNVI AQHPRGGERI TDIYSHLLAE RIVYLGTPID SGVANALIAQ LLHLESESPD 70 80 90 100 110 120 QEINFYINCE GGDLPSMLAV YDTMQHIGAP VHTTCVGQAI AVGAVLLAGG APGQRAMLPH 130 140 150 160 170 180 ARVVLHQPAA RGQGPIPDLI LQADELVRMR SEIEAILSRH TGRSPEQLRE DTDRDRVFTA 190 TAALEYGLID TVLSPRG

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56017.1.
RefSeq	YP_117381.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z0M4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109838.
GenomeReviews	Gene locus NFA_11720 in contig AP006618_GR.
KEGG	nfa:nfa11720.
NMPDR	fig|247156.1.peg.1204.
PATRIC	22731916. VBINocFar94200_1211.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	TTCVGQA.
ProtClustDB	CLSK2523669.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA11720-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. Divergent sequence. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. False negative. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_NOCFA

Accession

Primary (citable) accession number: Q5Z0M4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program