Reviewed, UniProtKB/Swiss-Prot Q5Z0G2 (HUTI_NOCFA)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	NFA_12340

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Imidazolonepropionase HAMAP MF_00372		PRO_0000306475
Sites
	Metal binding	70	1	Zinc or iron By similarity
	Metal binding	72	1	Zinc or iron By similarity
	Metal binding	227	1	Zinc or iron By similarity
	Metal binding	301	1	Zinc or iron By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z0G2-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D3B6F77810B9482C	FASTA	392	41,273
	10 20 30 40 50 60 MPTTALTGIG QLVTNDPALG EGPLGLRRDA AIVFEDGVVA WVGDSAHVPA TDTAHDLDGR 70 80 90 100 110 120 AVLPGFVESH SHLVFAGDRA EEFAARMSGR PYGAGGIRTT IEATRAATDE QLGANVRRLL 130 140 150 160 170 180 DESLRAGSTT VECKSGYGQS VEHELRSVRV AGRYTDEVTL LAAHVPPPEY AGRVDDYVAM 190 200 210 220 230 240 ACAEMIPRCA PHAKWIDVFC EQGAFDRDQA HAVLTAGIAH GLVPRVHGNQ LHRGPGVQLA 250 260 270 280 290 300 VEVGAASVDH VTYIDDADIE ALAHSDTVAT LLPGADFCTR NSYPDARALL DAGVTVALGA 310 320 330 340 350 360 DCNPGTSYTT SLPFCIALAV RELRMTPDEA VWAATAGGAR ALRRGDVGVL TPGARADALA 370 380 390 LDAPSHLHLA YRPGVPLISR VWREGTLAYA TN

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006618 Genomic DNA. Translation: BAD56079.1.
RefSeq	YP_117443.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3109583.
GenomeReviews	Gene locus NFA_12340 in contig AP006618_GR.
KEGG	nfa:nfa12340.
NMPDR	fig|247156.1.peg.1265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5Z0G2.
OMA	Q5Z0G2. MNMACTL.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA12340-MON.
BRENDA	3.5.2.7. 290697.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_NOCFA

Accession

Primary (citable) accession number: Q5Z0G2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	November 23, 2004
Last modified:	June 16, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)