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Q5Z0G2 (HUTI_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Imidazolonepropionase
EC=3.5.2.7
Alternative name(s):
Imidazolone-5-propionate hydrolase
Gene names
Name:hutI
Ordered Locus Names:NFA_12340
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372

Cofactor

Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372

Pathway

Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372

Subcellular location

Cytoplasm Potential HAMAP MF_00372.

Sequence similarities

Belongs to the HutI family.

Ontologies

Keywords
   Biological processHistidine metabolism
   Cellular componentCytoplasm
   LigandIron
Metal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processhistidine catabolic process to glutamate and formamide

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionimidazolonepropionase activity

Inferred from electronic annotation. Source: EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 392392Imidazolonepropionase HAMAP MF_00372
PRO_0000306475

Sites

Metal binding701Zinc or iron By similarity
Metal binding721Zinc or iron By similarity
Metal binding2271Zinc or iron By similarity
Metal binding3011Zinc or iron By similarity
Binding site791Substrate By similarity
Binding site921Substrate By similarity
Binding site1371Substrate By similarity
Binding site1641Substrate By similarity
Binding site2301Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5Z0G2 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: D3B6F77810B9482C

FASTA39241,273
        10         20         30         40         50         60 
MPTTALTGIG QLVTNDPALG EGPLGLRRDA AIVFEDGVVA WVGDSAHVPA TDTAHDLDGR 

        70         80         90        100        110        120 
AVLPGFVESH SHLVFAGDRA EEFAARMSGR PYGAGGIRTT IEATRAATDE QLGANVRRLL 

       130        140        150        160        170        180 
DESLRAGSTT VECKSGYGQS VEHELRSVRV AGRYTDEVTL LAAHVPPPEY AGRVDDYVAM 

       190        200        210        220        230        240 
ACAEMIPRCA PHAKWIDVFC EQGAFDRDQA HAVLTAGIAH GLVPRVHGNQ LHRGPGVQLA 

       250        260        270        280        290        300 
VEVGAASVDH VTYIDDADIE ALAHSDTVAT LLPGADFCTR NSYPDARALL DAGVTVALGA 

       310        320        330        340        350        360 
DCNPGTSYTT SLPFCIALAV RELRMTPDEA VWAATAGGAR ALRRGDVGVL TPGARADALA 

       370        380        390 
LDAPSHLHLA YRPGVPLISR VWREGTLAYA TN

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006618 Genomic DNA. Translation: BAD56079.1.
RefSeqYP_117443.1. NC_006361.1.

3D structure databases

HSSPHSSP built from PDB template 2GOK based on UniProtKB Q8U8Z6.
ProteinModelPortalQ5Z0G2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3109583.
GenomeReviewsGene locus NFA_12340 in contig AP006618_GR.
KEGGnfa:nfa12340.
NMPDRfig|247156.1.peg.1265.
PATRIC22732052. VBINocFar94200_1279.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG686142.
OMAMNMACTL.
ProtClustDBPRK14085.

Enzyme and pathway databases

BioCycNFAR247156:NFA12340-MONOMER.

Family and domain databases

HAMAPMF_00372. HutI.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]
KOK01468.
PANTHERPTHR22642. PTHR22642. 1 hit.
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMSSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMsTIGR01224. HutI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameHUTI_NOCFA
AccessionPrimary (citable) accession number: Q5Z0G2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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