Q5Z062 (CLPP3_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 3
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp 3

Gene names

Name:	clpP3
Ordered Locus Names:	NFA_13340

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	ATP-dependent Clp protease proteolytic subunit 3 HAMAP MF_00444		PRO_0000179606
Sites
	Active site	118	1	By similarity
	Active site	143	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5Z062 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1E8AF599C65C4F5D	FASTA	221	24,147
	10 20 30 40 50 60 MALIDPRAGL SGIAPSSPQA RYILPSFIEH SSFGVKESNP YNKLFEERII FLGVQVDDAS 70 80 90 100 110 120 ANDIMAQLLV LESLDPDRDI TMYINSPGGS FTSLMAIYDT MQYVRADVAT VCLGQAASAA 130 140 150 160 170 180 AVLLAAGTPG KRACLPNARV LIHQPSLEGG IQGQVSDLEI QAAEIERMRR LMETTLARHT 190 200 210 220 GKDPDTIRKD TDRDKILTAE DAKEYGIIDT VFDYRKLSAQ K

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56179.1.
RefSeq	YP_117543.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z062.
ModBase	Search...
Protein family/group databases
MEROPS	S14.009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109841.
GenomeReviews	Gene locus NFA_13340 in contig AP006618_GR.
KEGG	nfa:nfa13340.
NMPDR	fig|247156.1.peg.1363.
PATRIC	22732278. VBINocFar94200_1387.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	QDPYTKL.
ProtClustDB	PRK12553.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA13340-MONOMER.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP3_NOCFA

Accession

Primary (citable) accession number: Q5Z062

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program