Q5Z025 (PROA_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	NFA_13710

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate. HAMAP MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP MF_00412
Subcellular location	Cytoplasm By similarity HAMAP MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	Gamma-glutamyl phosphate reductase HAMAP MF_00412		PRO_0000189758

Sequences

	Sequence		Length	Mass (Da)
	Q5Z025 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 6A026BB649292A87	FASTA	421	44,021
	10 20 30 40 50 60 MTVETTAEFD VREAVHEAAR RARVASRALA QLTTAQKNDA LHAAADTLLA AADTVLAANA 70 80 90 100 110 120 EDIAAAEAAG TEASLLDRLR LTKARIDGIA SGLRQVAGLP DPVGGVVRGS TLPNGLEIRQ 130 140 150 160 170 180 VRVPLGVVGM VYEARPNVTV DAFGLALKSG NAALLRGSSS AAKSNAALVE ILREALRAQQ 190 200 210 220 230 240 IPADAVQLLP SHDRSSVTHL IQARGLVDVV IPRGGAGLIN AVVRDAIVPT IETGTGNCHV 250 260 270 280 290 300 YVHAAADLDM AEQILINAKT RRPSVCNAAE TVLIDRAVAD TAVPRLTAAL REHGVTIHGD 310 320 330 340 350 360 LPGLVPATDT DWGEEYLTLD IALKVVDNLD AAVEHINTWG TGHTEAIVTG DLAAAREFTS 370 380 390 400 410 420 RVDAAAVMVN ASTAFTDGEQ FGFGAEIGIS TQKLHARGPM ALPELTSTKW IVWGEGQIRP V

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56216.1.
RefSeq	YP_117580.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5Z025.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111391.
GenomeReviews	Gene locus NFA_13710 in contig AP006618_GR.
KEGG	nfa:nfa13710.
NMPDR	fig|247156.1.peg.1399.
PATRIC	22732358. VBINocFar94200_1427.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG318080.
OMA	QYPAACN.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA13710-MONOMER.
Family and domain databases
HAMAP	MF_00412. ProA. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 2 hits.
KO	K00147.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. ProA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_NOCFA

Accession

Primary (citable) accession number: Q5Z025

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program