Q5YYY7 (RSMH_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	NFA_17580

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108673
Regions
	Region	41 – 43	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	60	1	S-adenosyl-L-methionine By similarity
	Binding site	87	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	111	1	S-adenosyl-L-methionine By similarity
	Binding site	118	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YYY7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 53A02FD6487CF5DA	FASTA	324	35,039
	10 20 30 40 50 60 MNREQHGARH VPVLLERADE LLGPALTEPG AVYVDATLGL GGHAEHFLTR YPGLRLVGLD 70 80 90 100 110 120 RDTEALRLAG ERLAPFAERI TLVHTRYDGI ADALGQAGLP PTGSVSAILM DLGVSSMQLD 130 140 150 160 170 180 EAERGFAYSV DAPLDMRMDP TSGITAADVL NTYSHGDLAR VLKNYGEERF AGKIASEVIR 190 200 210 220 230 240 RRAHKPFETS GELVELLYAT IPAAARRTGG HPAKRTFQAL RVEVNGELDS LRAALPAALD 250 260 270 280 290 300 ALRVGGRIVV MSYQSLEDRV VKQELAARTS SRTPVDLPVE LPGMGPEFRL LTRGAEKAGE 310 320 REIAENPRAA PVRMRAAERI EAAS

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56604.1.
RefSeq	YP_117968.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YYY7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3107131.
GenomeReviews	Gene locus NFA_17580 in contig AP006618_GR.
KEGG	nfa:nfa17580.
NMPDR	fig|247156.1.peg.1771.
PATRIC	22733126. VBINocFar94200_1808.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	NPATRAF.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA17580-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_NOCFA

Accession

Primary (citable) accession number: Q5YYY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program