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Q5YYW9 (SYI_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:NFA_17760
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length1042 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02003

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02003

Cofactor

Zinc By similarity. HAMAP-Rule MF_02003

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02003

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02003.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02003

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10421042Isoleucine--tRNA ligase HAMAP-Rule MF_02003
PRO_0000098551

Regions

Motif59 – 6911"HIGH" region HAMAP-Rule MF_02003
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_02003

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YYW9 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: A0BEB64BBD448E4E

FASTA1,042116,567
        10         20         30         40         50         60 
MADDSNSAYP RVDYGAGTGA AFPDLERRVL EAWAADDTFR ASIENRSGAA EFVFYDGPPF 

        70         80         90        100        110        120 
ANGLPHYGHL LTGYVKDVIP RFQTMRGKRV DRRFGWDCHG LPAEIEAEKQ LGITDKSQID 

       130        140        150        160        170        180 
AMGLAEFNAA CKSSVLRYTG EWRDYVTRQA RWVDFDNDYK TLDLDFMESV MWAFKSLYDK 

       190        200        210        220        230        240 
GLIYQGFRVL PYSWYEQTPL SNQETRLDDA YKMRQDPAVT VDMVLSVPGE HPLRELDGAN 

       250        260        270        280        290        300 
ALIWTTTPWT LPSNLAIAVH PDVRYVHLRA ADGTRYVLAA ERVSHYSREF GEDATVLAEF 

       310        320        330        340        350        360 
EGAALVGLSY RPPFDFFLGH PNAHRVLAAD YVTTDSGTGV VHMAPAFGEE DMEVCSANDI 

       370        380        390        400        410        420 
ELVQPLDPGG RFTSMVPPYE GLMVFDANPV IIKDLKAAGK LLRHETIEHS YPHSWRSGQP 

       430        440        450        460        470        480 
LIYMAVPSWF VAVTKFRDRM VELNKQITWV PEHIRDGQFG KWLEGARDWN ISRNRYWGSP 

       490        500        510        520        530        540 
IPVWVSDDPA YPRVDVYGSL EELERDFGVR PTDLHRPAID QLTRPNPDDP TGRSMMRRVP 

       550        560        570        580        590        600 
EVLDCWFESG SMPYAQVHYP FENKEWFDSH FPGDFIVEYN GQTRGWFYTL HVLATALFDS 

       610        620        630        640        650        660 
PAFKTVAAHG IVLGDDGLKM SKSKGNYPDV NEVFDRDGSD AMRWFLMSSP ILRGGNLIVT 

       670        680        690        700        710        720 
ERGIREGVSH ALRPLWNAWT FLQLYASKPG EWRTDSTHVL DRYILAKLAQ TRDGMTEALE 

       730        740        750        760        770        780 
VYDIAGACEE LRTFADALTN WYVRRSRSRF WSEDRDAVDT LHTVLEVATR LAAPLLPLIS 

       790        800        810        820        830        840 
EVIWRGLTGG RSVHLADWPA AADLPADPEL VSTMDEVRTV CSTVLSLRKA KNLRVRLPLA 

       850        860        870        880        890        900 
EVTIAAPDAE RLAPYADIVA DEVNVKKVDL TTDVAVHGRF ELAVNARAAG PRLGKDVQRV 

       910        920        930        940        950        960 
IKAVKAGDWT ESADGVVSAA GITLLPEEYT QRLVAAEPES TAALPGNAGL VVLDSVVTEE 

       970        980        990       1000       1010       1020 
LEAEGWARDL VRELQETRKS LGLDVSDRIH VVLEVPEARR SWAQTHRDLI AGEILATSLE 

      1030       1040 
FGTAGEPAAE LAGGVRASVR KA 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD56622.1.
RefSeqYP_117986.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YYW9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa17760.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD56622; BAD56622; NFA_17760.
GeneID3109586.
KEGGnfa:nfa17760.
PATRIC22733160. VBINocFar94200_1825.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMADWNLSRS.
OrthoDBEOG644ZM1.
ProtClustDBPRK06039.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-1814-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02003. Ile_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_NOCFA
AccessionPrimary (citable) accession number: Q5YYW9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries