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Q5YYW9

- SYI_NOCFA

UniProt

Q5YYW9 - SYI_NOCFA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei622 – 6221ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciNFAR247156:GJ9T-1814-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:NFA_17760
    OrganismiNocardia farcinica (strain IFM 10152)
    Taxonomic identifieri247156 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
    ProteomesiUP000006820: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10421042Isoleucine--tRNA ligasePRO_0000098551Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi247156.nfa17760.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YYW9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi619 – 6235"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5YYW9-1 [UniParc]FASTAAdd to Basket

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    MADDSNSAYP RVDYGAGTGA AFPDLERRVL EAWAADDTFR ASIENRSGAA     50
    EFVFYDGPPF ANGLPHYGHL LTGYVKDVIP RFQTMRGKRV DRRFGWDCHG 100
    LPAEIEAEKQ LGITDKSQID AMGLAEFNAA CKSSVLRYTG EWRDYVTRQA 150
    RWVDFDNDYK TLDLDFMESV MWAFKSLYDK GLIYQGFRVL PYSWYEQTPL 200
    SNQETRLDDA YKMRQDPAVT VDMVLSVPGE HPLRELDGAN ALIWTTTPWT 250
    LPSNLAIAVH PDVRYVHLRA ADGTRYVLAA ERVSHYSREF GEDATVLAEF 300
    EGAALVGLSY RPPFDFFLGH PNAHRVLAAD YVTTDSGTGV VHMAPAFGEE 350
    DMEVCSANDI ELVQPLDPGG RFTSMVPPYE GLMVFDANPV IIKDLKAAGK 400
    LLRHETIEHS YPHSWRSGQP LIYMAVPSWF VAVTKFRDRM VELNKQITWV 450
    PEHIRDGQFG KWLEGARDWN ISRNRYWGSP IPVWVSDDPA YPRVDVYGSL 500
    EELERDFGVR PTDLHRPAID QLTRPNPDDP TGRSMMRRVP EVLDCWFESG 550
    SMPYAQVHYP FENKEWFDSH FPGDFIVEYN GQTRGWFYTL HVLATALFDS 600
    PAFKTVAAHG IVLGDDGLKM SKSKGNYPDV NEVFDRDGSD AMRWFLMSSP 650
    ILRGGNLIVT ERGIREGVSH ALRPLWNAWT FLQLYASKPG EWRTDSTHVL 700
    DRYILAKLAQ TRDGMTEALE VYDIAGACEE LRTFADALTN WYVRRSRSRF 750
    WSEDRDAVDT LHTVLEVATR LAAPLLPLIS EVIWRGLTGG RSVHLADWPA 800
    AADLPADPEL VSTMDEVRTV CSTVLSLRKA KNLRVRLPLA EVTIAAPDAE 850
    RLAPYADIVA DEVNVKKVDL TTDVAVHGRF ELAVNARAAG PRLGKDVQRV 900
    IKAVKAGDWT ESADGVVSAA GITLLPEEYT QRLVAAEPES TAALPGNAGL 950
    VVLDSVVTEE LEAEGWARDL VRELQETRKS LGLDVSDRIH VVLEVPEARR 1000
    SWAQTHRDLI AGEILATSLE FGTAGEPAAE LAGGVRASVR KA 1042
    Length:1,042
    Mass (Da):116,567
    Last modified:November 23, 2004 - v1
    Checksum:iA0BEB64BBD448E4E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD56622.1.
    RefSeqiWP_011208307.1. NC_006361.1.
    YP_117986.1. NC_006361.1.

    Genome annotation databases

    EnsemblBacteriaiBAD56622; BAD56622; NFA_17760.
    GeneIDi3109586.
    KEGGinfa:nfa17760.
    PATRICi22733160. VBINocFar94200_1825.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD56622.1 .
    RefSeqi WP_011208307.1. NC_006361.1.
    YP_117986.1. NC_006361.1.

    3D structure databases

    ProteinModelPortali Q5YYW9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 247156.nfa17760.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD56622 ; BAD56622 ; NFA_17760 .
    GeneIDi 3109586.
    KEGGi nfa:nfa17760.
    PATRICi 22733160. VBINocFar94200_1825.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci NFAR247156:GJ9T-1814-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IFM 10152.

    Entry informationi

    Entry nameiSYI_NOCFA
    AccessioniPrimary (citable) accession number: Q5YYW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3