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Q5YYW9

- SYI_NOCFA

UniProt

Q5YYW9 - SYI_NOCFA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-1814-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:NFA_17760
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10421042Isoleucine--tRNA ligasePRO_0000098551Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa17760.

Structurei

3D structure databases

ProteinModelPortaliQ5YYW9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YYW9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADDSNSAYP RVDYGAGTGA AFPDLERRVL EAWAADDTFR ASIENRSGAA
60 70 80 90 100
EFVFYDGPPF ANGLPHYGHL LTGYVKDVIP RFQTMRGKRV DRRFGWDCHG
110 120 130 140 150
LPAEIEAEKQ LGITDKSQID AMGLAEFNAA CKSSVLRYTG EWRDYVTRQA
160 170 180 190 200
RWVDFDNDYK TLDLDFMESV MWAFKSLYDK GLIYQGFRVL PYSWYEQTPL
210 220 230 240 250
SNQETRLDDA YKMRQDPAVT VDMVLSVPGE HPLRELDGAN ALIWTTTPWT
260 270 280 290 300
LPSNLAIAVH PDVRYVHLRA ADGTRYVLAA ERVSHYSREF GEDATVLAEF
310 320 330 340 350
EGAALVGLSY RPPFDFFLGH PNAHRVLAAD YVTTDSGTGV VHMAPAFGEE
360 370 380 390 400
DMEVCSANDI ELVQPLDPGG RFTSMVPPYE GLMVFDANPV IIKDLKAAGK
410 420 430 440 450
LLRHETIEHS YPHSWRSGQP LIYMAVPSWF VAVTKFRDRM VELNKQITWV
460 470 480 490 500
PEHIRDGQFG KWLEGARDWN ISRNRYWGSP IPVWVSDDPA YPRVDVYGSL
510 520 530 540 550
EELERDFGVR PTDLHRPAID QLTRPNPDDP TGRSMMRRVP EVLDCWFESG
560 570 580 590 600
SMPYAQVHYP FENKEWFDSH FPGDFIVEYN GQTRGWFYTL HVLATALFDS
610 620 630 640 650
PAFKTVAAHG IVLGDDGLKM SKSKGNYPDV NEVFDRDGSD AMRWFLMSSP
660 670 680 690 700
ILRGGNLIVT ERGIREGVSH ALRPLWNAWT FLQLYASKPG EWRTDSTHVL
710 720 730 740 750
DRYILAKLAQ TRDGMTEALE VYDIAGACEE LRTFADALTN WYVRRSRSRF
760 770 780 790 800
WSEDRDAVDT LHTVLEVATR LAAPLLPLIS EVIWRGLTGG RSVHLADWPA
810 820 830 840 850
AADLPADPEL VSTMDEVRTV CSTVLSLRKA KNLRVRLPLA EVTIAAPDAE
860 870 880 890 900
RLAPYADIVA DEVNVKKVDL TTDVAVHGRF ELAVNARAAG PRLGKDVQRV
910 920 930 940 950
IKAVKAGDWT ESADGVVSAA GITLLPEEYT QRLVAAEPES TAALPGNAGL
960 970 980 990 1000
VVLDSVVTEE LEAEGWARDL VRELQETRKS LGLDVSDRIH VVLEVPEARR
1010 1020 1030 1040
SWAQTHRDLI AGEILATSLE FGTAGEPAAE LAGGVRASVR KA
Length:1,042
Mass (Da):116,567
Last modified:November 23, 2004 - v1
Checksum:iA0BEB64BBD448E4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD56622.1.
RefSeqiWP_011208307.1. NC_006361.1.
YP_117986.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD56622; BAD56622; NFA_17760.
GeneIDi3109586.
KEGGinfa:nfa17760.
PATRICi22733160. VBINocFar94200_1825.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD56622.1 .
RefSeqi WP_011208307.1. NC_006361.1.
YP_117986.1. NC_006361.1.

3D structure databases

ProteinModelPortali Q5YYW9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 247156.nfa17760.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD56622 ; BAD56622 ; NFA_17760 .
GeneIDi 3109586.
KEGGi nfa:nfa17760.
PATRICi 22733160. VBINocFar94200_1825.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci NFAR247156:GJ9T-1814-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.

Entry informationi

Entry nameiSYI_NOCFA
AccessioniPrimary (citable) accession number: Q5YYW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 23, 2004
Last modified: November 26, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3