Q5YYU0 (Q5YYU0_NOCFA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

NFA_18050

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	577 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	242	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	279	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	375	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q5YYU0 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1D77BD8136BBB29D	FASTA	577	63,528
	10 20 30 40 50 60 MFEVWAPLPR TVRVEVDGVG HAMTRSPDGW WRAEVAAGEG ARYGFLLDDD PTVLPDPRSP 70 80 90 100 110 120 RQPDGVHGRS AVHALDPAAW TDADWTGRPL AGAVFYELHI GTFTPQGTFD AAIDRLDHLV 130 140 150 160 170 180 DLGVTVVEIM PVNAFDGTHN WGYDGVLWYA VHEGYGGPDG LQRFVNACHA RGLAVCLDVV 190 200 210 220 230 240 YNHLGPSGNY LPRFGPYLAS GRNTWGQSLN LDGAGSDHVR RYILDNALRW LRDFHIDALR 250 260 270 280 290 300 LDAVHALVDR TATHLLAELA AETERLSAHV RRPLSLIAES DLNDPRLITP RAAGGYGLTG 310 320 330 340 350 360 QWNDDLHHAV HTAVSGERQG YYADFGTLAC LAETLTSGFF HAGTYSSFRG RTHGRPLDRD 370 380 390 400 410 420 LIPGFRLLGY TCTHDQIGNR ALGDRPGAYL TDGQLAVKAA LVLCSPFTPM LFMGEEWGAR 430 440 450 460 470 480 TPFQFFTAHT DPQIGAATAA GRKAEFAEHG WTAEEVPDPQ DPATFERSKL DWSDLDDPDR 490 500 510 520 530 540 ARLLACYRAL LALRRGRPEL TDPSLRHVGV DYDEQARWLV LHRGSLHLVC NLSEDPVTVP 550 560 570 VTGRSLLYWE PPTLGPTATT VPAHSFTLLE DRPVGER

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56651.1.
RefSeq	YP_118015.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YYU0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3106208.
GenomeReviews	Gene locus NFA_18050 in contig AP006618_GR.
KEGG	nfa:nfa18050.
NMPDR	fig|247156.1.peg.1816.
PATRIC	22733220. VBINocFar94200_1855.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q5YYU0_NOCFA

Accession

Primary (citable) accession number: Q5YYU0

Entry history

Integrated into UniProtKB/TrEMBL:	November 23, 2004
Last sequence update:	November 23, 2004
Last modified:	December 14, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)