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Q5YYR3 (BIOB_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:NFA_18320
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Biotin synthase HAMAP-Rule MF_01694
PRO_0000381508

Sites

Metal binding731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1491Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2081Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2781Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YYR3 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: C37FE622D5AD63B4

FASTA33736,485
        10         20         30         40         50         60 
MTQAPVQTDI LEIAREQVLE RGEGLTQDQT LAVLRLGDDR LEELLGLAHE VRMKWCGPEV 

        70         80         90        100        110        120 
EVEGIISLKT GGCPEDCHFC SQSGLFQSPV RAAWLDIPSL VEAAKQTAKT GATEFCIVAA 

       130        140        150        160        170        180 
VRGPDARLMA QVAAGVEAIR NEVDIQVACS LGMLTQEQVD QLAAMGVHRY NHNLETAKSH 

       190        200        210        220        230        240 
FPNVVTTHTW EERWDTLRMV REAGMEVCCG GILGMGETLE QRAEFAAQLA ELEPDEVPLN 

       250        260        270        280        290        300 
FLNPRPGTPF GDLEVLPASE ALKAVAAFRL ALPRTILRFA GGREITLGDL GAKQGILGGI 

       310        320        330 
NAVIVGNYLT TLGRPAESDL DLLGELKMPI KALNETL 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD56678.1.
RefSeqYP_118042.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YYR3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa18320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD56678; BAD56678; NFA_18320.
GeneID3109829.
KEGGnfa:nfa18320.
PATRIC22733280. VBINocFar94200_1882.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMAETHHNID.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-1873-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NOCFA
AccessionPrimary (citable) accession number: Q5YYR3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways