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Q5YYR3

- BIOB_NOCFA

UniProt

Q5YYR3 - BIOB_NOCFA

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Protein

Biotin synthase

Gene

bioB

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi149 – 1491Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi208 – 2081Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi278 – 2781Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-1873-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:NFA_18320
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Biotin synthasePRO_0000381508Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa18320.

Structurei

3D structure databases

ProteinModelPortaliQ5YYR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiETHHNID.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5YYR3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQAPVQTDI LEIAREQVLE RGEGLTQDQT LAVLRLGDDR LEELLGLAHE
60 70 80 90 100
VRMKWCGPEV EVEGIISLKT GGCPEDCHFC SQSGLFQSPV RAAWLDIPSL
110 120 130 140 150
VEAAKQTAKT GATEFCIVAA VRGPDARLMA QVAAGVEAIR NEVDIQVACS
160 170 180 190 200
LGMLTQEQVD QLAAMGVHRY NHNLETAKSH FPNVVTTHTW EERWDTLRMV
210 220 230 240 250
REAGMEVCCG GILGMGETLE QRAEFAAQLA ELEPDEVPLN FLNPRPGTPF
260 270 280 290 300
GDLEVLPASE ALKAVAAFRL ALPRTILRFA GGREITLGDL GAKQGILGGI
310 320 330
NAVIVGNYLT TLGRPAESDL DLLGELKMPI KALNETL
Length:337
Mass (Da):36,485
Last modified:November 23, 2004 - v1
Checksum:iC37FE622D5AD63B4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD56678.1.
RefSeqiYP_118042.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD56678; BAD56678; NFA_18320.
GeneIDi3109829.
KEGGinfa:nfa18320.
PATRICi22733280. VBINocFar94200_1882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD56678.1 .
RefSeqi YP_118042.1. NC_006361.1.

3D structure databases

ProteinModelPortali Q5YYR3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 247156.nfa18320.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD56678 ; BAD56678 ; NFA_18320 .
GeneIDi 3109829.
KEGGi nfa:nfa18320.
PATRICi 22733280. VBINocFar94200_1882.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi ETHHNID.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci NFAR247156:GJ9T-1873-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.

Entry informationi

Entry nameiBIOB_NOCFA
AccessioniPrimary (citable) accession number: Q5YYR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 23, 2004
Last modified: October 1, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3