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Protein

Imidazole glycerol phosphate synthase subunit HisH

Gene

hisH

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to HisF for the synthesis of IGP and AICAR.UniRule annotation

Catalytic activityi

5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O.UniRule annotation

Pathwayi: L-histidine biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate.UniRule annotation
Proteins known to be involved in the 9 steps of the subpathway in this organism are:
  1. ATP phosphoribosyltransferase (hisG)
  2. Phosphoribosyl-ATP pyrophosphatase (hisE)
  3. Phosphoribosyl-AMP cyclohydrolase (hisI)
  4. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA)
  5. Imidazole glycerol phosphate synthase subunit HisH (hisH), Imidazole glycerol phosphate synthase subunit HisF (hisF)
  6. Imidazoleglycerol-phosphate dehydratase (hisB)
  7. Histidinol-phosphate aminotransferase (hisC)
  8. no protein annotated in this organism
  9. Histidinol dehydrogenase (hisD)
This subpathway is part of the pathway L-histidine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway L-histidine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei83NucleophileUniRule annotation1
Active sitei192UniRule annotation1
Active sitei194UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00031; UER00010.

Names & Taxonomyi

Protein namesi
Recommended name:
Imidazole glycerol phosphate synthase subunit HisHUniRule annotation (EC:2.4.2.-UniRule annotation)
Alternative name(s):
IGP synthase glutamine amidotransferase subunitUniRule annotation
IGP synthase subunit HisHUniRule annotation
ImGP synthase subunit HisHUniRule annotation
Short name:
IGPS subunit HisHUniRule annotation
Gene namesi
Name:hisHUniRule annotation
Ordered Locus Names:NFA_18480
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002317411 – 211Imidazole glycerol phosphate synthase subunit HisHAdd BLAST211

Interactioni

Subunit structurei

Heterodimer of HisH and HisF.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa18480.

Structurei

3D structure databases

ProteinModelPortaliQ5YYP7.
SMRiQ5YYP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 211Glutamine amidotransferase type-1UniRule annotationAdd BLAST207

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UJE. Bacteria.
COG0118. LUCA.
KOiK02501.
OMAiWVYFVHS.
OrthoDBiPOG091H03NT.

Family and domain databases

CDDicd01748. GATase1_IGP_Synthase. 1 hit.
Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00278. HisH. 1 hit.
InterProiView protein in InterPro
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010139. Imidazole-glycPsynth_HisH.
PfamiView protein in Pfam
PF00117. GATase. 1 hit.
PIRSFiPIRSF000495. Amidotransf_hisH. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01855. IMP_synth_hisH. 1 hit.
PROSITEiView protein in PROSITE
PS51273. GATASE_TYPE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5YYP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRKSVALLD YGSGNLHSAE RALVRAGADV RVTADPDAAL AVDGLVVPGV
60 70 80 90 100
GAFAACMAGL RAVRGEKIIG QRLAGGRPVL GICVGMQILF ERGVEFGVET
110 120 130 140 150
EGCAEWPGVV ERLDAPVLPH MGWNTVSAPA DSVLFAGMDA DTRFYFVHSY
160 170 180 190 200
AAQRWELPPN EHFAAPKLTW AEHGVRFLAA VENGPLSATQ FHPEKSGDAG
210
AQLLRNWVDS L
Length:211
Mass (Da):22,476
Last modified:November 23, 2004 - v1
Checksum:i297D2A531CC576ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD56694.1.
RefSeqiWP_011208379.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD56694; BAD56694; NFA_18480.
KEGGinfa:NFA_18480.

Similar proteinsi

Entry informationi

Entry nameiHIS5_NOCFA
AccessioniPrimary (citable) accession number: Q5YYP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 23, 2004
Last modified: June 7, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways