Q5YYH7 (SYFA_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	NFA_19180

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000232002
Sites
	Metal binding	260	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YYH7 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D9D4539465F8355E	FASTA	342	37,257
	10 20 30 40 50 60 MDLSEEALAA AAADAEKAFA AAADLDALAV AKTEHLGGKA PLALAQRALG ALPKEEKKDA 70 80 90 100 110 120 GKRVNVARGR VAEAFEARRA VLLAERDAAV LVAEAIDVTL PARRQQVGAR HPITVISERI 130 140 150 160 170 180 ADVFVAMGWE VAEGPEVETE HFNFDALNFL PDHPARTMQD TFHIAPEGSR QVLRTHTSPV 190 200 210 220 230 240 QVRSMLERDL PIYVVCPGRT FRTDELDATH TPVFSQVEGL AVDKGLTMAH LRGTLDAFAR 250 260 270 280 290 300 ALFGPETRTR MRPNYFPFTE PSAEVDVWFP DKKGGAGWVE WGGCGMVNPK VLIASGIDPE 310 320 330 340 VYSGFAFGMG LERTLQFRNG IPDMRDIVEG DVRFTLPFGI QS

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56764.1.
RefSeq	YP_118128.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YYH7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111369.
GenomeReviews	Gene locus NFA_19180 in contig AP006618_GR.
KEGG	nfa:nfa19180.
NMPDR	fig|247156.1.peg.1924.
PATRIC	22733464. VBINocFar94200_1973.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA19180-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_NOCFA

Accession

Primary (citable) accession number: Q5YYH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program