Reviewed, UniProtKB/Swiss-Prot Q5YYF8 (ARGJ_NOCFA)

Names and origin

Protein names

Recommended name:
    Arginine biosynthesis bifunctional protein argJ
Cleaved into the following 2 chains:
    1- Recommended name:
            Arginine biosynthesis bifunctional protein argJ alpha chain
    2- Recommended name:
            Arginine biosynthesis bifunctional protein argJ beta chain
Including the following 2 domains:
    1- Recommended name:
            Glutamate N-acetyltransferase
              EC=2.3.1.35
        Alternative name(s):
            Ornithine acetyltransferase
              Short name=OATase
            Ornithine transacetylase
    2- Recommended name:
            Amino-acid acetyltransferase
              EC=2.3.1.1
        Alternative name(s):
            N-acetylglutamate synthase
              Short name=AGS

Gene names

Name:	argJ
Ordered Locus Names:	NFA_19370

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity. HAMAP MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity. HAMAP MF_01106
Subcellular location	Cytoplasm Probable HAMAP MF_01106.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 200	200	Arginine biosynthesis bifunctional protein argJ alpha chain By similarity		PRO_0000227240
	Chain	201 – 405	205	Arginine biosynthesis bifunctional protein argJ beta chain By similarity		PRO_0000227241
Sites
	Site	200 – 201	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YYF8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 7BDD4A46F496A4F8	FASTA	405	41,798
	10 20 30 40 50 60 MTTTDSGTGK LVRTQGVTAP LGFRAAGIAA GIKASGKPDL ALVFNEGPEY AAAGVFTRNK 70 80 90 100 110 120 VKAAPVLWSQ QVLTSRRLRA VILNSGGANA CTGPEGFQDT HRTAEELARA LSNWGTETGA 130 140 150 160 170 180 GEIAVCSTGL IGDRLPMDKL IPAVTEIVHE MGGGLSGGTD AAHAIMTTDT VPKEAAFHHR 190 200 210 220 230 240 DKWNVGGMAK GAGMLAPSLA TMLVVLTTDA AVTAEQLDTA LRKATRLTFD RLDVDGSCST 250 260 270 280 290 300 NDTVLLLANG ASEVTPSQEE LEAAVFAVCD DLAAQLMADA EGVTKRVRVT VTGAANEDEA 310 320 330 340 350 360 VTAARAIARD SLVKTALFGS DPNWGRVLAA VGIAPITLDP DRISVSFNGN PVCVNGVGAP 370 380 390 400 GARQVDLSGA DIDVLVELNV GDGEAMIRTT DLSHGYVEEN SAYSS

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD56783.1.
RefSeq	YP_118147.1.
3D structure databases
SMR	Q5YYF8. Positions 12-200, 19-401, 201-403.
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	3109863.
GenomeReviews	Gene locus NFA_19370 in contig AP006618_GR.
KEGG	nfa:nfa19370.
NMPDR	fig|247156.1.peg.1942.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284202.
OMA	VHENSAY.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA19370-MONOMER.
BRENDA	2.3.1.1. 290697. 2.3.1.35. 290697.
Family and domain databases
HAMAP	MF_01106. ArgJ. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_NOCFA

Accession

Primary (citable) accession number: Q5YYF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)