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Q5YXD6

- BLAC_NOCFA

UniProt

Q5YXD6 - BLAC_NOCFA

Protein

Beta-lactamase FAR-1

Gene

bla

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 2 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Also hydrolyzes aztreonam at a low level. Not active against ceftazidime, cefotaxime and imipenem.

    Catalytic activityi

    A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

    Enzyme regulationi

    Inhibited by clavulanic acid, and at a low level by tazobactam and sulbactam.1 Publication

    Kineticsi

    1. KM=30 µM for benzylpenicillin1 Publication
    2. KM=50 µM for amoxicillin1 Publication
    3. KM=31 µM for ticarcillin1 Publication
    4. KM=45 µM for piperacillin1 Publication
    5. KM=104 µM for cephalothin1 Publication
    6. KM=400 µM for aztreonam1 Publication

    Vmax=5.5 µmol/sec/mg enzyme with benzylpenicillin as substrate1 Publication

    Vmax=3.8 µmol/sec/mg enzyme with amoxicillin as substrate1 Publication

    Vmax=1.6 µmol/sec/mg enzyme with ticarcillin as substrate1 Publication

    Vmax=9.2 µmol/sec/mg enzyme with piperacillin as substrate1 Publication

    Vmax=0.13 µmol/sec/mg enzyme with cephalothin as substrate1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei94 – 941Acyl-ester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. beta-lactamase activity Source: UniProtKB-EC

    GO - Biological processi

    1. beta-lactam antibiotic catabolic process Source: InterPro
    2. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance

    Enzyme and pathway databases

    BioCyciNFAR247156:GJ9T-2355-MONOMER.
    SABIO-RKQ5YXD6.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-lactamase FAR-1 (EC:3.5.2.6)
    Gene namesi
    Name:bla
    Synonyms:far1
    Ordered Locus Names:NFA_23080
    OrganismiNocardia farcinica (strain IFM 10152)
    Taxonomic identifieri247156 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
    ProteomesiUP000006820: Chromosome

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 313285Beta-lactamase FAR-1PRO_0000313798Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi29 – 291N-palmitoyl cysteineSequence Analysis
    Lipidationi29 – 291S-diacylglycerol cysteineSequence Analysis

    Keywords - PTMi

    Lipoprotein, Palmitate

    Interactioni

    Protein-protein interaction databases

    STRINGi247156.nfa23080.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YXD6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni258 – 2603Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the class-A beta-lactamase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2367.
    KOiK17836.
    OMAiGETHDTS.
    OrthoDBiEOG6K9QFS.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view]
    PRINTSiPR00118. BLACTAMASEA.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5YXD6-1 [UniParc]FASTAAdd to Basket

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    MPGVDISFLK KSGRRTMAAA AVIALLGGCG ADAGSEPATT AASTTAPSAA    50
    TDAATAEFAA LEQRFGARLG VYAVDTTSGA VVAYRADERF GMASTFKGLA 100
    CGALLREHPL SSGYFDQVVR YSREEVVSYS PVTETRVDTG MTVAELCHAT 150
    ITVSDNTAGN QILKLLGGPA GFTAFLRSLG DEVSRLDRWE TELNEVPPGE 200
    ERDTTTPAAV AANYRALVLG DVLAEPERAQ LRDWLVANTT GDQRIRAGVP 250
    AGWTVGDKTG GGSHGGNNDV AVAWTETGDP IVIALLSHRT DPAAKADNAL 300
    LAEATRAVVT ALR 313
    Length:313
    Mass (Da):32,566
    Last modified:January 15, 2008 - v2
    Checksum:iEB60AB1DC46B841D
    GO

    Sequence cautioni

    The sequence AAB81957.1 differs from that shown. Reason: Frameshift at position 2.
    The sequence BAD57155.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221V → A in AAB81957. (PubMed:10390216)Curated
    Sequence conflicti49 – 491A → T in AAB81957. (PubMed:10390216)Curated
    Sequence conflicti65 – 651F → S in AAB81957. (PubMed:10390216)Curated
    Sequence conflicti81 – 811V → E in AAB81957. (PubMed:10390216)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
    AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
    RefSeqiYP_118519.1. NC_006361.1.

    Genome annotation databases

    EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
    GeneIDi3106090.
    KEGGinfa:nfa23080.
    PATRICi22734236. VBINocFar94200_2355.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF024601 Genomic DNA. Translation: AAB81957.1 . Frameshift.
    AP006618 Genomic DNA. Translation: BAD57155.1 . Different initiation.
    RefSeqi YP_118519.1. NC_006361.1.

    3D structure databases

    ProteinModelPortali Q5YXD6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 247156.nfa23080.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD57155 ; BAD57155 ; NFA_23080 .
    GeneIDi 3106090.
    KEGGi nfa:nfa23080.
    PATRICi 22734236. VBINocFar94200_2355.

    Phylogenomic databases

    eggNOGi COG2367.
    KOi K17836.
    OMAi GETHDTS.
    OrthoDBi EOG6K9QFS.

    Enzyme and pathway databases

    BioCyci NFAR247156:GJ9T-2355-MONOMER.
    SABIO-RK Q5YXD6.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000871. Beta-lactam_class-A/D.
    IPR023650. Beta-lactam_class-A_AS.
    [Graphical view ]
    PRINTSi PR00118. BLACTAMASEA.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Biochemical-genetic analysis and distribution of FAR-1, a class A beta-lactamase from Nocardia farcinica."
      Laurent F., Poirel L., Naas T., Chaibi E.B., Labia R., Boiron P., Nordmann P.
      Antimicrob. Agents Chemother. 43:1644-1650(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: VIC.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IFM 10152.
    3. Cited for: EVIDENCE OF MEMBRANE-BOUND LOCALIZATION.

    Entry informationi

    Entry nameiBLAC_NOCFA
    AccessioniPrimary (citable) accession number: Q5YXD6
    Secondary accession number(s): O30987
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 67 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3