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Protein

Beta-lactamase FAR-1

Gene

bla

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Also hydrolyzes aztreonam at a low level. Not active against ceftazidime, cefotaxime and imipenem.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid, and at a low level by tazobactam and sulbactam.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin1 Publication
  3. KM=31 µM for ticarcillin1 Publication
  4. KM=45 µM for piperacillin1 Publication
  5. KM=104 µM for cephalothin1 Publication
  6. KM=400 µM for aztreonam1 Publication
  1. Vmax=5.5 µmol/sec/mg enzyme with benzylpenicillin as substrate1 Publication
  2. Vmax=3.8 µmol/sec/mg enzyme with amoxicillin as substrate1 Publication
  3. Vmax=1.6 µmol/sec/mg enzyme with ticarcillin as substrate1 Publication
  4. Vmax=9.2 µmol/sec/mg enzyme with piperacillin as substrate1 Publication
  5. Vmax=0.13 µmol/sec/mg enzyme with cephalothin as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941Acyl-ester intermediateBy similarity
Sitei97 – 971Increases nucleophilicity of active site SerBy similarity
Binding sitei154 – 1541SubstrateBy similarity
Active sitei190 – 1901Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-2355-MONOMER.
SABIO-RKQ5YXD6.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase FAR-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:far1
Ordered Locus Names:NFA_23080
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 313285Beta-lactamase FAR-1PRO_0000313798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi29 – 291N-palmitoyl cysteineSequence Analysis
Lipidationi29 – 291S-diacylglycerol cysteineSequence Analysis

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi247156.nfa23080.

Structurei

3D structure databases

ProteinModelPortaliQ5YXD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni258 – 2603Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2367.
KOiK17836.
OMAiKTYAAGA.
OrthoDBiEOG6K9QFS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5YXD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVDISFLK KSGRRTMAAA AVIALLGGCG ADAGSEPATT AASTTAPSAA
60 70 80 90 100
TDAATAEFAA LEQRFGARLG VYAVDTTSGA VVAYRADERF GMASTFKGLA
110 120 130 140 150
CGALLREHPL SSGYFDQVVR YSREEVVSYS PVTETRVDTG MTVAELCHAT
160 170 180 190 200
ITVSDNTAGN QILKLLGGPA GFTAFLRSLG DEVSRLDRWE TELNEVPPGE
210 220 230 240 250
ERDTTTPAAV AANYRALVLG DVLAEPERAQ LRDWLVANTT GDQRIRAGVP
260 270 280 290 300
AGWTVGDKTG GGSHGGNNDV AVAWTETGDP IVIALLSHRT DPAAKADNAL
310
LAEATRAVVT ALR
Length:313
Mass (Da):32,566
Last modified:January 15, 2008 - v2
Checksum:iEB60AB1DC46B841D
GO

Sequence cautioni

The sequence AAB81957.1 differs from that shown. Reason: Frameshift at position 2. Curated
The sequence BAD57155.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221V → A in AAB81957 (PubMed:10390216).Curated
Sequence conflicti49 – 491A → T in AAB81957 (PubMed:10390216).Curated
Sequence conflicti65 – 651F → S in AAB81957 (PubMed:10390216).Curated
Sequence conflicti81 – 811V → E in AAB81957 (PubMed:10390216).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqiWP_011208840.1. NC_006361.1.
YP_118519.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
KEGGinfa:nfa23080.
PATRICi22734236. VBINocFar94200_2355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqiWP_011208840.1. NC_006361.1.
YP_118519.1. NC_006361.1.

3D structure databases

ProteinModelPortaliQ5YXD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi247156.nfa23080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
KEGGinfa:nfa23080.
PATRICi22734236. VBINocFar94200_2355.

Phylogenomic databases

eggNOGiCOG2367.
KOiK17836.
OMAiKTYAAGA.
OrthoDBiEOG6K9QFS.

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-2355-MONOMER.
SABIO-RKQ5YXD6.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical-genetic analysis and distribution of FAR-1, a class A beta-lactamase from Nocardia farcinica."
    Laurent F., Poirel L., Naas T., Chaibi E.B., Labia R., Boiron P., Nordmann P.
    Antimicrob. Agents Chemother. 43:1644-1650(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: VIC.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.
  3. Cited for: EVIDENCE OF MEMBRANE-BOUND LOCALIZATION.

Entry informationi

Entry nameiBLAC_NOCFA
AccessioniPrimary (citable) accession number: Q5YXD6
Secondary accession number(s): O30987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: May 27, 2015
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.