Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5YXD6

- BLAC_NOCFA

UniProt

Q5YXD6 - BLAC_NOCFA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-lactamase FAR-1

Gene

bla

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Also hydrolyzes aztreonam at a low level. Not active against ceftazidime, cefotaxime and imipenem.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid, and at a low level by tazobactam and sulbactam.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin1 Publication
  3. KM=31 µM for ticarcillin1 Publication
  4. KM=45 µM for piperacillin1 Publication
  5. KM=104 µM for cephalothin1 Publication
  6. KM=400 µM for aztreonam1 Publication

Vmax=5.5 µmol/sec/mg enzyme with benzylpenicillin as substrate1 Publication

Vmax=3.8 µmol/sec/mg enzyme with amoxicillin as substrate1 Publication

Vmax=1.6 µmol/sec/mg enzyme with ticarcillin as substrate1 Publication

Vmax=9.2 µmol/sec/mg enzyme with piperacillin as substrate1 Publication

Vmax=0.13 µmol/sec/mg enzyme with cephalothin as substrate1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei94 – 941Acyl-ester intermediateBy similarity
Sitei97 – 971Increases nucleophilicity of active site SerBy similarity
Binding sitei154 – 1541SubstrateBy similarity
Active sitei190 – 1901Proton acceptorBy similarity

GO - Molecular functioni

  1. beta-lactamase activity Source: UniProtKB-EC

GO - Biological processi

  1. beta-lactam antibiotic catabolic process Source: InterPro
  2. response to antibiotic Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-2355-MONOMER.
SABIO-RKQ5YXD6.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase FAR-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:far1
Ordered Locus Names:NFA_23080
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820: Chromosome

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828Sequence AnalysisAdd
BLAST
Chaini29 – 313285Beta-lactamase FAR-1PRO_0000313798Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi29 – 291N-palmitoyl cysteineSequence Analysis
Lipidationi29 – 291S-diacylglycerol cysteineSequence Analysis

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi247156.nfa23080.

Structurei

3D structure databases

ProteinModelPortaliQ5YXD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni258 – 2603Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2367.
KOiK17836.
OMAiGETHDTS.
OrthoDBiEOG6K9QFS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5YXD6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPGVDISFLK KSGRRTMAAA AVIALLGGCG ADAGSEPATT AASTTAPSAA
60 70 80 90 100
TDAATAEFAA LEQRFGARLG VYAVDTTSGA VVAYRADERF GMASTFKGLA
110 120 130 140 150
CGALLREHPL SSGYFDQVVR YSREEVVSYS PVTETRVDTG MTVAELCHAT
160 170 180 190 200
ITVSDNTAGN QILKLLGGPA GFTAFLRSLG DEVSRLDRWE TELNEVPPGE
210 220 230 240 250
ERDTTTPAAV AANYRALVLG DVLAEPERAQ LRDWLVANTT GDQRIRAGVP
260 270 280 290 300
AGWTVGDKTG GGSHGGNNDV AVAWTETGDP IVIALLSHRT DPAAKADNAL
310
LAEATRAVVT ALR
Length:313
Mass (Da):32,566
Last modified:January 15, 2008 - v2
Checksum:iEB60AB1DC46B841D
GO

Sequence cautioni

The sequence AAB81957.1 differs from that shown. Reason: Frameshift at position 2. Curated
The sequence BAD57155.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221V → A in AAB81957. (PubMed:10390216)Curated
Sequence conflicti49 – 491A → T in AAB81957. (PubMed:10390216)Curated
Sequence conflicti65 – 651F → S in AAB81957. (PubMed:10390216)Curated
Sequence conflicti81 – 811V → E in AAB81957. (PubMed:10390216)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqiYP_118519.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
GeneIDi3106090.
KEGGinfa:nfa23080.
PATRICi22734236. VBINocFar94200_2355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1 . Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1 . Different initiation.
RefSeqi YP_118519.1. NC_006361.1.

3D structure databases

ProteinModelPortali Q5YXD6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 247156.nfa23080.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD57155 ; BAD57155 ; NFA_23080 .
GeneIDi 3106090.
KEGGi nfa:nfa23080.
PATRICi 22734236. VBINocFar94200_2355.

Phylogenomic databases

eggNOGi COG2367.
KOi K17836.
OMAi GETHDTS.
OrthoDBi EOG6K9QFS.

Enzyme and pathway databases

BioCyci NFAR247156:GJ9T-2355-MONOMER.
SABIO-RK Q5YXD6.

Family and domain databases

Gene3Di 3.40.710.10. 1 hit.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view ]
PRINTSi PR00118. BLACTAMASEA.
SUPFAMi SSF56601. SSF56601. 1 hit.
PROSITEi PS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Biochemical-genetic analysis and distribution of FAR-1, a class A beta-lactamase from Nocardia farcinica."
    Laurent F., Poirel L., Naas T., Chaibi E.B., Labia R., Boiron P., Nordmann P.
    Antimicrob. Agents Chemother. 43:1644-1650(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: VIC.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.
  3. Cited for: EVIDENCE OF MEMBRANE-BOUND LOCALIZATION.

Entry informationi

Entry nameiBLAC_NOCFA
AccessioniPrimary (citable) accession number: Q5YXD6
Secondary accession number(s): O30987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3