Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Beta-lactamase FAR-1

Gene

bla

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Confers high levels of resistance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Also hydrolyzes aztreonam at a low level. Not active against ceftazidime, cefotaxime and imipenem.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by clavulanic acid, and at a low level by tazobactam and sulbactam.1 Publication

Kineticsi

  1. KM=30 µM for benzylpenicillin1 Publication
  2. KM=50 µM for amoxicillin1 Publication
  3. KM=31 µM for ticarcillin1 Publication
  4. KM=45 µM for piperacillin1 Publication
  5. KM=104 µM for cephalothin1 Publication
  6. KM=400 µM for aztreonam1 Publication
  1. Vmax=5.5 µmol/sec/mg enzyme with benzylpenicillin as substrate1 Publication
  2. Vmax=3.8 µmol/sec/mg enzyme with amoxicillin as substrate1 Publication
  3. Vmax=1.6 µmol/sec/mg enzyme with ticarcillin as substrate1 Publication
  4. Vmax=9.2 µmol/sec/mg enzyme with piperacillin as substrate1 Publication
  5. Vmax=0.13 µmol/sec/mg enzyme with cephalothin as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94Acyl-ester intermediateBy similarity1
Sitei97Increases nucleophilicity of active site SerBy similarity1
Binding sitei154SubstrateBy similarity1
Active sitei190Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Enzyme and pathway databases

SABIO-RKQ5YXD6.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase FAR-1 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:far1
Ordered Locus Names:NFA_23080
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000031379829 – 313Beta-lactamase FAR-1Add BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi29N-palmitoyl cysteineSequence analysis1
Lipidationi29S-diacylglycerol cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Palmitate

Interactioni

Protein-protein interaction databases

STRINGi247156.nfa23080.

Structurei

3D structure databases

ProteinModelPortaliQ5YXD6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni258 – 260Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
KOiK17836.
OMAiMMSTFKT.
OrthoDBiPOG091H023N.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5YXD6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGVDISFLK KSGRRTMAAA AVIALLGGCG ADAGSEPATT AASTTAPSAA
60 70 80 90 100
TDAATAEFAA LEQRFGARLG VYAVDTTSGA VVAYRADERF GMASTFKGLA
110 120 130 140 150
CGALLREHPL SSGYFDQVVR YSREEVVSYS PVTETRVDTG MTVAELCHAT
160 170 180 190 200
ITVSDNTAGN QILKLLGGPA GFTAFLRSLG DEVSRLDRWE TELNEVPPGE
210 220 230 240 250
ERDTTTPAAV AANYRALVLG DVLAEPERAQ LRDWLVANTT GDQRIRAGVP
260 270 280 290 300
AGWTVGDKTG GGSHGGNNDV AVAWTETGDP IVIALLSHRT DPAAKADNAL
310
LAEATRAVVT ALR
Length:313
Mass (Da):32,566
Last modified:January 15, 2008 - v2
Checksum:iEB60AB1DC46B841D
GO

Sequence cautioni

The sequence AAB81957 differs from that shown. Reason: Frameshift at position 2.Curated
The sequence BAD57155 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22V → A in AAB81957 (PubMed:10390216).Curated1
Sequence conflicti49A → T in AAB81957 (PubMed:10390216).Curated1
Sequence conflicti65F → S in AAB81957 (PubMed:10390216).Curated1
Sequence conflicti81V → E in AAB81957 (PubMed:10390216).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqiWP_041560070.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
KEGGinfa:NFA_23080.
PATRICi22734236. VBINocFar94200_2355.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqiWP_041560070.1. NC_006361.1.

3D structure databases

ProteinModelPortaliQ5YXD6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi247156.nfa23080.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD57155; BAD57155; NFA_23080.
KEGGinfa:NFA_23080.
PATRICi22734236. VBINocFar94200_2355.

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
KOiK17836.
OMAiMMSTFKT.
OrthoDBiPOG091H023N.

Enzyme and pathway databases

SABIO-RKQ5YXD6.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLAC_NOCFA
AccessioniPrimary (citable) accession number: Q5YXD6
Secondary accession number(s): O30987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.