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Q5YXD6 (BLAC_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Beta-lactamase FAR-1
EC=3.5.2.6
Gene names
Name:bla
Synonyms:far1
Ordered Locus Names:NFA_23080
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Confers high levels of resitance to amoxicillin, benzylpenicillin, piperacillin, ticarcillin and cephalothin. Also hydrolyzes aztreonam at a low level. Not active against ceftazidime, cefotaxime and imipenem.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Inhibited by clavulanic acid, and at a low level by tazobactam and sulbactam. Ref.1

Subcellular location

Cell membrane; Lipid-anchor Probable.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Biophysicochemical properties

Kinetic parameters:

KM=30 µM for benzylpenicillin Ref.1

KM=50 µM for amoxicillin

KM=31 µM for ticarcillin

KM=45 µM for piperacillin

KM=104 µM for cephalothin

KM=400 µM for aztreonam

Vmax=5.5 µmol/sec/mg enzyme with benzylpenicillin as substrate

Vmax=3.8 µmol/sec/mg enzyme with amoxicillin as substrate

Vmax=1.6 µmol/sec/mg enzyme with ticarcillin as substrate

Vmax=9.2 µmol/sec/mg enzyme with piperacillin as substrate

Vmax=0.13 µmol/sec/mg enzyme with cephalothin as substrate

Sequence caution

The sequence AAB81957.1 differs from that shown. Reason: Frameshift at position 2.

The sequence BAD57155.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentCell membrane
Membrane
   DomainSignal
   Molecular functionHydrolase
   PTMLipoprotein
Palmitate
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionbeta-lactamase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 313285Beta-lactamase FAR-1
PRO_0000313798

Regions

Region258 – 2603Substrate binding By similarity

Sites

Active site941Acyl-ester intermediate By similarity

Amino acid modifications

Lipidation291N-palmitoyl cysteine Potential
Lipidation291S-diacylglycerol cysteine Potential

Experimental info

Sequence conflict221V → A in AAB81957. Ref.1
Sequence conflict491A → T in AAB81957. Ref.1
Sequence conflict651F → S in AAB81957. Ref.1
Sequence conflict811V → E in AAB81957. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5YXD6 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: EB60AB1DC46B841D

FASTA31332,566
        10         20         30         40         50         60 
MPGVDISFLK KSGRRTMAAA AVIALLGGCG ADAGSEPATT AASTTAPSAA TDAATAEFAA 

        70         80         90        100        110        120 
LEQRFGARLG VYAVDTTSGA VVAYRADERF GMASTFKGLA CGALLREHPL SSGYFDQVVR 

       130        140        150        160        170        180 
YSREEVVSYS PVTETRVDTG MTVAELCHAT ITVSDNTAGN QILKLLGGPA GFTAFLRSLG 

       190        200        210        220        230        240 
DEVSRLDRWE TELNEVPPGE ERDTTTPAAV AANYRALVLG DVLAEPERAQ LRDWLVANTT 

       250        260        270        280        290        300 
GDQRIRAGVP AGWTVGDKTG GGSHGGNNDV AVAWTETGDP IVIALLSHRT DPAAKADNAL 

       310 
LAEATRAVVT ALR

« Hide

References

« Hide 'large scale' references
[1]"Biochemical-genetic analysis and distribution of FAR-1, a class A beta-lactamase from Nocardia farcinica."
Laurent F., Poirel L., Naas T., Chaibi E.B., Labia R., Boiron P., Nordmann P.
Antimicrob. Agents Chemother. 43:1644-1650(1999) [PubMed: 10390216] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: VIC.
[2]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.
[3]"Partial characterization of Nocardia farcinica beta-lactamases."
Steingrube V.A., Wallace R.J., Brown B.A., Zhang Y., Steele L.C., Young G., Nash D.R.
Antimicrob. Agents Chemother. 37:1850-1855(1993) [PubMed: 8239595] [Abstract]
Cited for: EVIDENCE OF MEMBRANE-BOUND LOCALIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF024601 Genomic DNA. Translation: AAB81957.1. Frameshift.
AP006618 Genomic DNA. Translation: BAD57155.1. Different initiation.
RefSeqYP_118519.1. NC_006361.1.

3D structure databases

HSSPHSSP built from PDB template 2GDN based on UniProtKB P0A5I6.
ProteinModelPortalQ5YXD6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3106090.
GenomeReviewsGene locus NFA_23080 in contig AP006618_GR.
KEGGnfa:nfa23080.
NMPDRfig|247156.1.peg.2286.
PATRIC22734236. VBINocFar94200_2355.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG535713.
OMAATYDNQL.
ProtClustDBCLSK711795.

Enzyme and pathway databases

BioCycNFAR247156:NFA23080-MONOMER.

Family and domain databases

InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
Gene3DG3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
KOK01467.
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. PBP_transp_fold. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAC_NOCFA
AccessionPrimary (citable) accession number: Q5YXD6
Secondary accession number(s): O30987
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: January 25, 2012
This is version 50 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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