Reviewed, UniProtKB/Swiss-Prot Q5YX61 (ILVD2_NOCFA)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	NFA_23830

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	629 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 629	629	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000225402
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5YX61-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 663037E1E3625E20	FASTA	629	65,710
	10 20 30 40 50 60 MPELRSRTVT HGRNMAGARA LMRASGVPAA DIGAKPVVAV ANSFTEFVPG HTHLQPVGRI 70 80 90 100 110 120 VGDAIRRAGG IPREFNTIAV DDGIAMGHQG MLYSLPSRDL IADSIEYMVQ AHCADALVCI 130 140 150 160 170 180 SNCDKITPGM LLAAMRLDIP TVFVSGGPME GGRATLADGT VRRLDLITAM SEAVNDATSD 190 200 210 220 230 240 ADLATIEENA CPTCGSCAGM FTANSMNCLV EALGLALPGN GTTLATHTAR RDLYEAAGAT 250 260 270 280 290 300 IMAITRRYYD RDDATVLPRA IASRAAFDNA MALDLAMGGS TNTVLHLLAA AHEAGLDYTL 310 320 330 340 350 360 ADIEKRSRAV PCLCKVAPNG SHLMEDVHRA GGIPAILGEL RRGGHLHTTV RAVHSESLDG 370 380 390 400 410 420 WLAEWDVRGP NPAQAAVDLF HAAPGGVRSA TAFSQSARWA ALDLDAESGC IRDVAHAYSE 430 440 450 460 470 480 DGGLAVLRGN LAVDGAVVKS AGVPADLHVF TGEAVVAESQ EEAVTAVLSG RVRPGTVLVI 490 500 510 520 530 540 RYEGPRGGPG MQEMLYPTAY LKGRGLAGSV AVVTDGRFSG GSSGLSIGHV SPEAAAGGTI 550 560 570 580 590 600 AAVTDGDPIT IDIPSRTLRL EVDDAEIARR LAHRRRTGYR PRSRHRPLST ALRAYALLAQ 610 620 SADKGGVRRL PPDELGGPEA AFDTQTRAG

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006618 Genomic DNA. Translation: BAD57230.1.
RefSeq	YP_118594.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3111186.
GenomeReviews	Gene locus NFA_23830 in contig AP006618_GR.
KEGG	nfa:nfa23830.
NMPDR	fig|247156.1.peg.2350.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5YX61.
OMA	NSHNEII.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA23830-MON.
BRENDA	4.2.1.9. 290697.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_NOCFA

Accession

Primary (citable) accession number: Q5YX61

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)