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Q5YWU1

- MASZ_NOCFA

UniProt

Q5YWU1 - MASZ_NOCFA

Protein

Malate synthase G

Gene

glcB

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei632 – 6321Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciNFAR247156:GJ9T-2550-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:NFA_25030
    OrganismiNocardia farcinica (strain IFM 10152)
    Taxonomic identifieri247156 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
    ProteomesiUP000006820: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 726726Malate synthase GPRO_1000056915Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei618 – 6181Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi247156.nfa25030.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YWU1.
    SMRiQ5YWU1. Positions 2-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    KOiK01638.
    OMAiSTIMDFE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5YWU1-1 [UniParc]FASTAAdd to Basket

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    MTERIQVGGL QVAKVLHDFI ENEALPGSGV DSAAFWAGAE QVITDLAPRN    50
    RALLAERDEI QGKLDAWHAE HPGANYDKAA YKSFLTEIGY LRPEPADFQI 100
    TTQNVDSEIA ETAGPQLVVP VMNARFAINA ANARWGSLYD ALYGTDAIPE 150
    DNGAEKGTGY NKVRGDKVIE WARNFLDDAV TLITGSHIGS TSYAIVDGEL 200
    EVGLEDGTTI GLADASQLVG YQGDPAKPTS ILLKHNGLHI DIQIDPESPI 250
    GSTDTAGIKD IVLESAVTTI MDFEDSVAAV DAEDKVLCYH NWLGLMQGTL 300
    AEEVSKGGKT FTRTMNPDRV YTALDGSELR LHGRSLLFVR NVGHLMTSDA 350
    ILDAQGNEVP EGIMDGLITS LIAKHSLRED VELKNSRTGS VYIVKPKMHG 400
    PDEVAFTNEL FGRIEDVLGL ARNTLKVGIM DEERRTTVNL KACIEAAKER 450
    VVFINTGFLD RTGDEIHTSM EAGPMVRKAD MKSQQWIASY EDWNVDTGLA 500
    TGLPGKAQIG KGMWAMPDLM ADMLAQKIGH PKSGANTAWV PSPTAATLHA 550
    THYHLVDVFK RQAEIAKGGR RASVDEILEI PLAANPNWSP EEIRQELDNN 600
    SQSILGYVVR WIDQGVGCSK VPDIKDVALM EDRATLRISS QLMANWLRHG 650
    IVTADEVVAS LERMAPVVDR QNAGDPNYRP MAPDFDGSIA FQAAKELILE 700
    GTKQPNGYTE PILHRRRREA KALSRV 726
    Length:726
    Mass (Da):79,260
    Last modified:November 23, 2004 - v1
    Checksum:i633308E4D3C79077
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD57350.1.
    RefSeqiWP_011209035.1. NC_006361.1.
    YP_118714.1. NC_006361.1.

    Genome annotation databases

    EnsemblBacteriaiBAD57350; BAD57350; NFA_25030.
    GeneIDi3109647.
    KEGGinfa:nfa25030.
    PATRICi22734632. VBINocFar94200_2553.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD57350.1 .
    RefSeqi WP_011209035.1. NC_006361.1.
    YP_118714.1. NC_006361.1.

    3D structure databases

    ProteinModelPortali Q5YWU1.
    SMRi Q5YWU1. Positions 2-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 247156.nfa25030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD57350 ; BAD57350 ; NFA_25030 .
    GeneIDi 3109647.
    KEGGi nfa:nfa25030.
    PATRICi 22734632. VBINocFar94200_2553.

    Phylogenomic databases

    eggNOGi COG2225.
    KOi K01638.
    OMAi STIMDFE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci NFAR247156:GJ9T-2550-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IFM 10152.

    Entry informationi

    Entry nameiMASZ_NOCFA
    AccessioniPrimary (citable) accession number: Q5YWU1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3