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Q5YWU1 (MASZ_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:NFA_25030
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia ›

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 726726Malate synthase G HAMAP-Rule MF_00641
PRO_1000056915

Sites

Active site3401Proton acceptor By similarity
Active site6321Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YWU1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 633308E4D3C79077

FASTA72679,260
        10         20         30         40         50         60 
MTERIQVGGL QVAKVLHDFI ENEALPGSGV DSAAFWAGAE QVITDLAPRN RALLAERDEI 

        70         80         90        100        110        120 
QGKLDAWHAE HPGANYDKAA YKSFLTEIGY LRPEPADFQI TTQNVDSEIA ETAGPQLVVP 

       130        140        150        160        170        180 
VMNARFAINA ANARWGSLYD ALYGTDAIPE DNGAEKGTGY NKVRGDKVIE WARNFLDDAV 

       190        200        210        220        230        240 
TLITGSHIGS TSYAIVDGEL EVGLEDGTTI GLADASQLVG YQGDPAKPTS ILLKHNGLHI 

       250        260        270        280        290        300 
DIQIDPESPI GSTDTAGIKD IVLESAVTTI MDFEDSVAAV DAEDKVLCYH NWLGLMQGTL 

       310        320        330        340        350        360 
AEEVSKGGKT FTRTMNPDRV YTALDGSELR LHGRSLLFVR NVGHLMTSDA ILDAQGNEVP 

       370        380        390        400        410        420 
EGIMDGLITS LIAKHSLRED VELKNSRTGS VYIVKPKMHG PDEVAFTNEL FGRIEDVLGL 

       430        440        450        460        470        480 
ARNTLKVGIM DEERRTTVNL KACIEAAKER VVFINTGFLD RTGDEIHTSM EAGPMVRKAD 

       490        500        510        520        530        540 
MKSQQWIASY EDWNVDTGLA TGLPGKAQIG KGMWAMPDLM ADMLAQKIGH PKSGANTAWV 

       550        560        570        580        590        600 
PSPTAATLHA THYHLVDVFK RQAEIAKGGR RASVDEILEI PLAANPNWSP EEIRQELDNN 

       610        620        630        640        650        660 
SQSILGYVVR WIDQGVGCSK VPDIKDVALM EDRATLRISS QLMANWLRHG IVTADEVVAS 

       670        680        690        700        710        720 
LERMAPVVDR QNAGDPNYRP MAPDFDGSIA FQAAKELILE GTKQPNGYTE PILHRRRREA 


KALSRV

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006618 Genomic DNA. Translation: BAD57350.1.
RefSeqYP_118714.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YWU1.
SMRQ5YWU1. Positions 2-722.
ModBaseSearch...

Protein-protein interaction databases

STRING247156.nfa25030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD57350; BAD57350; NFA_25030.
GeneID3109647.
KEGGnfa:nfa25030.
PATRIC22734632. VBINocFar94200_2553.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
KOK01638.
OMAVVKPKMH.
ProtClustDBPRK02999.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-2550-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_NOCFA
AccessionPrimary (citable) accession number: Q5YWU1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: May 29, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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