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Q5YWU1 (MASZ_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G

EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:NFA_25030
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length726 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity. HAMAP-Rule MF_00641

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   PTMOxidation
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 726726Malate synthase G HAMAP-Rule MF_00641
PRO_1000056915

Regions

Region125 – 1262Acetyl-CoA binding By similarity
Region457 – 4604Glyoxylate binding By similarity

Sites

Active site3401Proton acceptor By similarity
Active site6321Proton donor By similarity
Metal binding4321Magnesium By similarity
Metal binding4601Magnesium By similarity
Binding site1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding site2761Acetyl-CoA By similarity
Binding site3131Acetyl-CoA By similarity
Binding site3401Glyoxylate By similarity
Binding site4321Glyoxylate By similarity
Binding site5411Acetyl-CoA; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue6181Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YWU1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 633308E4D3C79077

FASTA72679,260
        10         20         30         40         50         60 
MTERIQVGGL QVAKVLHDFI ENEALPGSGV DSAAFWAGAE QVITDLAPRN RALLAERDEI 

        70         80         90        100        110        120 
QGKLDAWHAE HPGANYDKAA YKSFLTEIGY LRPEPADFQI TTQNVDSEIA ETAGPQLVVP 

       130        140        150        160        170        180 
VMNARFAINA ANARWGSLYD ALYGTDAIPE DNGAEKGTGY NKVRGDKVIE WARNFLDDAV 

       190        200        210        220        230        240 
TLITGSHIGS TSYAIVDGEL EVGLEDGTTI GLADASQLVG YQGDPAKPTS ILLKHNGLHI 

       250        260        270        280        290        300 
DIQIDPESPI GSTDTAGIKD IVLESAVTTI MDFEDSVAAV DAEDKVLCYH NWLGLMQGTL 

       310        320        330        340        350        360 
AEEVSKGGKT FTRTMNPDRV YTALDGSELR LHGRSLLFVR NVGHLMTSDA ILDAQGNEVP 

       370        380        390        400        410        420 
EGIMDGLITS LIAKHSLRED VELKNSRTGS VYIVKPKMHG PDEVAFTNEL FGRIEDVLGL 

       430        440        450        460        470        480 
ARNTLKVGIM DEERRTTVNL KACIEAAKER VVFINTGFLD RTGDEIHTSM EAGPMVRKAD 

       490        500        510        520        530        540 
MKSQQWIASY EDWNVDTGLA TGLPGKAQIG KGMWAMPDLM ADMLAQKIGH PKSGANTAWV 

       550        560        570        580        590        600 
PSPTAATLHA THYHLVDVFK RQAEIAKGGR RASVDEILEI PLAANPNWSP EEIRQELDNN 

       610        620        630        640        650        660 
SQSILGYVVR WIDQGVGCSK VPDIKDVALM EDRATLRISS QLMANWLRHG IVTADEVVAS 

       670        680        690        700        710        720 
LERMAPVVDR QNAGDPNYRP MAPDFDGSIA FQAAKELILE GTKQPNGYTE PILHRRRREA 


KALSRV 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD57350.1.
RefSeqYP_118714.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YWU1.
SMRQ5YWU1. Positions 2-722.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa25030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD57350; BAD57350; NFA_25030.
GeneID3109647.
KEGGnfa:nfa25030.
PATRIC22734632. VBINocFar94200_2553.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
KOK01638.
OMASTIMDFE.
OrthoDBEOG6HJ286.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-2550-MONOMER.
UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_NOCFA
AccessionPrimary (citable) accession number: Q5YWU1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways