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Q5YVL0 (MSHC_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Short name=L-Cys:GlcN-Ins ligase
EC=6.3.1.13
Alternative name(s):
Mycothiol ligase
Short name=MSH ligase
Gene names
Name:mshC
Synonyms:cysS2
Ordered Locus Names:NFA_29340
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins By similarity. HAMAP MF_01697

Catalytic activity

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate. HAMAP MF_01697

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01697

Subunit structure

Monomer By similarity. HAMAP MF_01697

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 412412L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase HAMAP MF_01697
PRO_0000159447

Regions

Region43 – 464Cysteinyl adenylate binding By similarity
Region81 – 833Cysteinyl adenylate binding By similarity
Region249 – 2513Cysteinyl adenylate binding By similarity
Motif45 – 5511"HIGH" region HAMAP MF_01697
Motif187 – 1926"ERGGDP" region HAMAP MF_01697
Motif289 – 2935"KMSKS" region HAMAP MF_01697

Sites

Metal binding431Zinc By similarity
Metal binding2311Zinc By similarity
Metal binding2561Zinc By similarity
Binding site581Cysteinyl adenylate By similarity
Binding site2271Cysteinyl adenylate By similarity
Binding site2831Cysteinyl adenylate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YVL0 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: D3AF3A381E8B3262

FASTA41244,914
        10         20         30         40         50         60 
MQSWSDTALP TVPGAGPPLR LYDTADRQVR PVAPGATATM YVCGITPYDA THLGHAATYL 

        70         80         90        100        110        120 
TFDLVNRVLR DGGHDVHYVQ NVTDVDDPLF ERAARDGVDW RELGSREIEL FRTDMAALRV 

       130        140        150        160        170        180 
LPPREYVGAV ESVEEVVEFV QKLLANGAAY VVDDPDFPDI YFRTDATEQF GYESGYDRAT 

       190        200        210        220        230        240 
MERLFAERGG DPDRPGKRDP LDALLWRAAR PGEPSWPAPF GAGRPGWHIE CSAIALNRLG 

       250        260        270        280        290        300 
PEFDIQGGGS DLIYPHHEYS AAHAESVVAG RRFARHYVHA GLIGLDGEKM SKSRGNLVLV 

       310        320        330        340        350        360 
STLRKDGVDP AAIRLGLLDG HYRQDRMWTD AVLEAALARL ARWRSATALS AGPAAHDTIA 

       370        380        390        400        410 
RLRQHLADDL DSPKALAAVD NWVTEALDYG GSDSGAPAAI AEAVDALLGV RL

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006618 Genomic DNA. Translation: BAD57781.1.
RefSeqYP_119145.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YVL0.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3109802.
GenomeReviewsGene locus NFA_29340 in contig AP006618_GR.
KEGGnfa:nfa29340.
NMPDRfig|247156.1.peg.2874.
PATRIC22735472. VBINocFar94200_2972.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG327651.
OMAALFREDM.
ProtClustDBPRK12418.

Enzyme and pathway databases

BioCycNFAR247156:NFA29340-MONOMER.

Family and domain databases

HAMAPMF_01697. MshC.
[Tree]
InterProIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK15526.
PANTHERPTHR10890. Cys_tRNA-synt_1a. 1 hit.
PfamPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
TIGRFAMsTIGR03447. Mycothiol_MshC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMSHC_NOCFA
AccessionPrimary (citable) accession number: Q5YVL0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: November 23, 2004
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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