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Protein

Catalase-peroxidase

Gene

katG

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity.UniRule annotation

Catalytic activityi

Donor + H2O2 = oxidized donor + 2 H2O.UniRule annotation
2 H2O2 = O2 + 2 H2O.UniRule annotation

Cofactori

heme bUniRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per dimer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei103 – 1031Transition state stabilizerUniRule annotation
Active sitei107 – 1071Proton acceptorUniRule annotation
Metal bindingi270 – 2701Iron (heme axial ligand)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-2999-MONOMER.

Protein family/group databases

PeroxiBasei2380. NfaCP01_IFM10152.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-peroxidaseUniRule annotation (EC:1.11.1.21UniRule annotation)
Short name:
CPUniRule annotation
Alternative name(s):
Peroxidase/catalaseUniRule annotation
Gene namesi
Name:katGUniRule annotation
Ordered Locus Names:NFA_29500
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 739739Catalase-peroxidasePRO_0000354851Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki106 ↔ 229Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-255)UniRule annotation
Cross-linki229 ↔ 255Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-106)UniRule annotation

Post-translational modificationi

The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme.UniRule annotation

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa29500.

Structurei

3D structure databases

ProteinModelPortaliQ5YVJ4.
SMRiQ5YVJ4. Positions 26-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Peroxidase/catalase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
KOiK03782.
OMAiTESKCPF.
OrthoDBiEOG6RRKKM.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YVJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEHPPIGE ANTEPAAGGC PVTGRLRHPL QGGGNHEWWP NQLNLKVLAK
60 70 80 90 100
NPAEGNPLGD FDYKAAFNSL DLAAVKADIA EVLTTSQDWW PADFGNYGPL
110 120 130 140 150
MIRMAWHSAG TYRSSDGRGG ANTGQQRFAP LNSWPDNGNL DKARRLLWPV
160 170 180 190 200
KKKYGQNISW ADLMILAGNV ALETMGFKTF GFAGGRVDVW EPEEDVYWGP
210 220 230 240 250
EAEWLGDKRY SGERDLENPL AAVQMGLIYV NPEGPNGNPD PLAAAVDIKD
260 270 280 290 300
TFGRMGMTVE ETVALIAGGH TFGKTHGAGD AALVGAEPEA APLEQMGLGW
310 320 330 340 350
KSSHGTGKGA DAITSGLEVV WTTKPTQWSN DFFEILFGYE WELTKSPAGA
360 370 380 390 400
NQWVAKDAQP IIPDPFDPAK KRLPTMLTTD LSLRVDPEME VISRRFKDNP
410 420 430 440 450
DEFADAFARA WFKLTHRDLG PVTRYLGPEV PSEVQLWQDP IPAVDHELVD
460 470 480 490 500
AADIAALKEQ ILASELSISQ LVKTAWAAAS SFRGSDYRGG ANGGRIRLQP
510 520 530 540 550
QLGWEVNEPD ELARVIAVLE GIQEQFNAAQ TGNKKVSFAD LVVLGGVAAV
560 570 580 590 600
EQAARNAGVA VEVPFTPGRA DTTQELTDPE GFAVLEPKAD GFRNYLGKAN
610 620 630 640 650
PLPAEYLLVD KANLLTLTAP EMTVLVGGLR VLNANYQRSP LGVLTETPES
660 670 680 690 700
LTNDFFVNLL DMGVVWEPSP ADDGTYVGKD AATGAQKWTG SRVDLLFGSN
710 720 730
SVLRSLAEVY ATDDAKPKFV QDFVAAWNKV MNLDRFDLA
Length:739
Mass (Da):80,210
Last modified:November 23, 2004 - v1
Checksum:i51B7275E2C4651CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD57797.1.
RefSeqiWP_011209482.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD57797; BAD57797; NFA_29500.
KEGGinfa:NFA_29500.
PATRICi22735504. VBINocFar94200_2988.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD57797.1.
RefSeqiWP_011209482.1. NC_006361.1.

3D structure databases

ProteinModelPortaliQ5YVJ4.
SMRiQ5YVJ4. Positions 26-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi247156.nfa29500.

Protein family/group databases

PeroxiBasei2380. NfaCP01_IFM10152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD57797; BAD57797; NFA_29500.
KEGGinfa:NFA_29500.
PATRICi22735504. VBINocFar94200_2988.

Phylogenomic databases

eggNOGiENOG4105C1X. Bacteria.
COG0376. LUCA.
KOiK03782.
OMAiTESKCPF.
OrthoDBiEOG6RRKKM.

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-2999-MONOMER.

Family and domain databases

HAMAPiMF_01961. Catal_peroxid.
InterProiIPR000763. Catalase_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 2 hits.
[Graphical view]
PRINTSiPR00460. BPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 2 hits.
TIGRFAMsiTIGR00198. cat_per_HPI. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.

Entry informationi

Entry nameiKATG_NOCFA
AccessioniPrimary (citable) accession number: Q5YVJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 23, 2004
Last modified: March 16, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.