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Q5YTI1 (MDH_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:NFA_36620
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Malate dehydrogenase HAMAP-Rule MF_01517
PRO_0000113383

Regions

Nucleotide binding16 – 227NAD By similarity
Nucleotide binding134 – 1363NAD By similarity

Sites

Active site1921Proton acceptor By similarity
Binding site971Substrate By similarity
Binding site1031Substrate By similarity
Binding site1101NAD By similarity
Binding site1171NAD By similarity
Binding site1361Substrate By similarity
Binding site1671Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YTI1 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: C79493FA1D8CCE8C

FASTA33434,910
        10         20         30         40         50         60 
MSTADRTAPV TVTVTGAAGQ IAYGLLFRIA SGAMLGPHTP IRLRLLEIPA AVASLEGVAM 

        70         80         90        100        110        120 
ELEDGAFPLL DAIDISDDPW TGFAGANVAL LVGARPRTAG MERADLLAAN GPIFTDQGQA 

       130        140        150        160        170        180 
INAVAADDVK VLVVGNPANT NAFIAMSNAP DVPAERFTAM TRLDHNRAIA QLAKKTGAPA 

       190        200        210        220        230        240 
TEIHRIAVWG NHSATQYPDL THATVAGRPA RELVDEQWLR EDFIPTVQQR GTAIIQARGA 

       250        260        270        280        290        300 
SSAASAASAA IDHIHDWVLG TPAGEWTSMA VPSDGSYGVP DGLISSFPVT CADGAYRIVE 

       310        320        330 
GLEVDAFSRE RIDASVAELT AEREAVVELG FAKG 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD58510.1.
RefSeqYP_119874.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YTI1.
SMRQ5YTI1. Positions 8-331.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa36620.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD58510; BAD58510; NFA_36620.
GeneID3111271.
KEGGnfa:nfa36620.
PATRIC22736946. VBINocFar94200_3709.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0039.
KOK00024.
OMAVNDQAWL.
OrthoDBEOG6PP9Q2.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-3712-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDH_NOCFA
AccessionPrimary (citable) accession number: Q5YTI1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 23, 2004
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families