Reviewed, UniProtKB/Swiss-Prot Q5YT43 (LEXA2_NOCFA)

Names and origin

Protein names

Recommended name:
    LexA repressor 2
    EC=3.4.21.88

Gene names

Name:	lexA2
Ordered Locus Names:	NFA_38000

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	LexA repressor 2 HAMAP MF_00015		PRO_0000170061
Regions
	DNA binding	48 – 68	21	H-T-H motif By similarity
Sites
	Active site	167	1	For autocatalytic cleavage activity By similarity
	Active site	204	1	For autocatalytic cleavage activity By similarity
	Site	132 – 133	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YT43-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 9212D48BF9016B79	FASTA	243	26,102
	10 20 30 40 50 60 MSRTDDTGEE SGVAVDPALN GADLTVRQRK VLEVIRTSVS ERGYPPSIRE IGDAVGLTST 70 80 90 100 110 120 SSVAHQLRAL ERKGYLRRDP NRPRAVDVRG LDEAVRAVTA LPGAALEEPD TLAEDTGRPT 130 140 150 160 170 180 PTFVPVLGRI AAGGPILAEQ AVEDVFPLPR ELVGDGSLFL LRVVGQSMVD AAICDGDWVV 190 200 210 220 230 240 VRQQNVAENG DIVAAMIDGE ATVKTFKRTG KDVWLMPHNP LFEPIPGNDA RILGKVVTVI RKI

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006618 Genomic DNA. Translation: BAD58648.1.
RefSeq	YP_120012.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	3111217.
GenomeReviews	Gene locus NFA_38000 in contig AP006618_GR.
KEGG	nfa:nfa38000.
NMPDR	fig|247156.1.peg.3701.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5YT43.
OMA	KVIGVFR.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA38000-MON.
BRENDA	3.4.21.88. 290697.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA2_NOCFA

Accession

Primary (citable) accession number: Q5YT43

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	November 23, 2004
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)