Q5YSB9 (SYP_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	NFA_40730

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity. HAMAP MF_01569
Subcellular location	Cytoplasm By similarity HAMAP MF_01569.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Proline--tRNA ligase HAMAP MF_01569		PRO_0000248732

Sequences

	Sequence		Length	Mass (Da)
	Q5YSB9 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: D66020AD50444187	FASTA	585	63,550
	10 20 30 40 50 60 MITRLSHLFL RTLRDDPADA EVPSHKLLVR AGYVRRVAPG VYSWLPLGLR VLRKVEDVVR 70 80 90 100 110 120 AEMNAIGGQE ISLPALLPRD PYETTNRWTE YGDSLFRLHD RKGADYLLGP THEELFALTV 130 140 150 160 170 180 KGEYNSYKDL PVTLYQIQTK YRDEERPRAG ILRGREFVMK DSYSFDLDED GLKASYNAHR 190 200 210 220 230 240 EAYQRIFDRL GVKYVIVAAT SGAMGGSASE EFLADSPVGE DTYVICRESG YAANVEAVVT 250 260 270 280 290 300 PAPEPQPIEG RPAAVVHDTP DTPTIASLVE WANAAGIAEQ FGRPVTAADT LKNVMVKLRH 310 320 330 340 350 360 PDGKTEIVGI GVPGDREVDD KRLGASVEPA EVELLTDADF AANPFLVKGY IGPKALQENG 370 380 390 400 410 420 IRYLVDPRVG TGTAWITGAD SPGKHVVGLI AGRDFTPDGT IEAAEVRDGD PSPDGRGTLE 430 440 450 460 470 480 AARGIEIGHI FQLGYKYTDA FEVDVLGENG KPVRLIMGSY GIGVSRMVAV IAEQQHDEKG 490 500 510 520 530 540 LRWPAAVAPF DVHVVVANKD EAARTGAEQV VAGLDAQGLD VLFDDRTASP GVKFKDAELL 550 560 570 580 GMPWVVVIGR GWADGTVELR NRFTGEAENV PAAEAVATVV ARIRG

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD58922.1.
RefSeq	YP_120286.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YSB9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111394.
GenomeReviews	Gene locus NFA_40730 in contig AP006618_GR.
KEGG	nfa:nfa40730.
NMPDR	fig|247156.1.peg.3932.
PATRIC	22737686. VBINocFar94200_4072.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403504.
OMA	IQPAELW.
ProtClustDB	PRK09194.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA40730-MONOMER.
Family and domain databases
HAMAP	MF_01569. Pro_tRNA_synth_type1. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004500. Pro-tRNA-synth_IIa_bac-type. IPR023717. Pro-tRNA-Synthase_IIa_type1. IPR007214. YbaK/aa-tRNA-synth-assoc-dom. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMs	TIGR00409. ProS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_NOCFA

Accession

Primary (citable) accession number: Q5YSB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program