Q5YS72 (DXR_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose 5-phosphate reductoisomerase
Short name=DXP reductoisomerase
EC=1.1.1.267

Alternative name(s):
1-deoxyxylulose-5-phosphate reductoisomerase
2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	NFA_41200

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00183
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity. HAMAP MF_00183
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: EC NADPH binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_0000163685
Regions
	Nucleotide binding	25 – 54	30	NADP By similarity
Sites
	Metal binding	157	1	Divalent metal cation By similarity
	Metal binding	159	1	Divalent metal cation By similarity
	Metal binding	228	1	Divalent metal cation By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	159	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	206	1	Substrate By similarity
	Binding site	228	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YS72 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 6BBD590CC879AE70	FASTA	394	41,055
	10 20 30 40 50 60 MRPPSRCPGM RHNDGVSDIV RVLLLGSTGS IGTQALEVIA ANPDKFEVVG LAARGGNPAL 70 80 90 100 110 120 LAEQMAATGT RNVAVADPAA GAALDIKLAG PHAVTELVRR TEADVVLNAL VGSLGLEPTL 130 140 150 160 170 180 ATLTAGTRLA LANKESLVAG GSLVTRAAAP GQIVPVDSEH SALAQCLRGG RADEVDRLVL 190 200 210 220 230 240 TASGGPFRGW TAQMLESVNP EAAKAHPTWS MGLMNTLNSA SLVNKGLELI ETHLLFGIPY 250 260 270 280 290 300 DRIDVTVHPQ SIVHSMVTFV DGSTLAQASP PDMKLPIALA LGWPDRVPGA AAACDFSTAS 310 320 330 340 350 360 TWTFEPVDTE VFPAVELARQ AGQAGGSVTA VYNAANEVAV QAFLDGRIRF PEIVRTVARA 370 380 390 VEAADRWRAE PETVEDVLAA DRWARGRAAE LVGA

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD58969.1.
RefSeq	YP_120333.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YS72.
SMR	Q5YS72. Positions 20-392.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109765.
GenomeReviews	Gene locus NFA_41200 in contig AP006618_GR.
KEGG	nfa:nfa41200.
NMPDR	fig|247156.1.peg.3977.
PATRIC	22737778. VBINocFar94200_4118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG430762.
OMA	IHSMVEY.
ProtClustDB	PRK05447.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA41200-MONOMER.
Family and domain databases
HAMAP	MF_00183. DXP_reductoisom. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00099.
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_NOCFA

Accession

Primary (citable) accession number: Q5YS72

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program