Q5YS47 (RNH2_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	NFA_41450

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Ribonuclease HII HAMAP MF_00052_B		PRO_0000235743
Sites
	Metal binding	38	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	132	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YS47 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: E9A010B615720CB2	FASTA	251	26,572
	10 20 30 40 50 60 MTARTGWPPR MVMRKAGGLR TLEAALIRGG LGPVAGVDEA GRGPCAGPLV VAACLLAPKA 70 80 90 100 110 120 YDRLAGLDDS KKLTEAAREE LYPVITRLAL AWEVVVIPAW EIDAIGIHVA NIEGMRRAVA 130 140 150 160 170 180 GLRQRPGYVL TDGFRVPGLA APSLPVIGGD AAAACIAAAS ILAKVTRDRI MVELDSRHPG 190 200 210 220 230 240 YGFAAHKGYN TPEHTAALQR LGPCSEHRRS WRNVRERLGL RPLDPTVEYA ETVLADGVAD 250 SRVAADAAHA G

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD58994.1.
RefSeq	YP_120358.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YS47.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111464.
GenomeReviews	Gene locus NFA_41450 in contig AP006618_GR.
KEGG	nfa:nfa41450.
NMPDR	fig|247156.1.peg.3999.
PATRIC	22737822. VBINocFar94200_4140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA41450-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_NOCFA

Accession

Primary (citable) accession number: Q5YS47

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program