Q5YRX6 (SYE_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	NFA_42160

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 489	489	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119615
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	254 – 258	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	257	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YRX6 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 1D9403992B960FBD	FASTA	489	54,251
	10 20 30 40 50 60 MTEVRVRFCP SPTGTPHVGL IRTALFNWAY ARHTGGTFVF RIEDTDAARD SEESYHAILD 70 80 90 100 110 120 ALRWLGLTWD EGPEVGGPYG PYRQSQRREL YLDVVRRLLE AGEAYESFST PEEVEARHRA 130 140 150 160 170 180 AGRDPKLGYD NYDRDLTPEA IEAYRAAGRP AVVRLRMPDE DLTWTDLVRG ETTFKAGTVP 190 200 210 220 230 240 DFALTRGNGD PLYTLVNPVD DASMKITHVL RGEDLLSSTP RQLALYAALR RIGVADFTPE 250 260 270 280 290 300 FGHLPFVMGQ GNKKLSKRDP ESNLFAHRDR GFIPEGLLNY LALLGWSLAD DRDVFSMSEM 310 320 330 340 350 360 VAAFDISKVN SNPARFDQKK ADALNAEHIR LLEPGDFAHR LRQFLTEHGH IGADVDEKVF 370 380 390 400 410 420 AAAAELVQTR IVVLGDAWDL LRFLFAPADE FTVDEAAAAK NLGADAVPVL EAAIAALEPL 430 440 450 460 470 480 PGWATPVIED ALKTALIDDL GLKPRKAFAP VRVAVTGSHI SPPLYESLEL LGRELTLARL RSGLEWARQ

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD59065.1.
RefSeq	YP_120429.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YRX6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3109603.
GenomeReviews	Gene locus NFA_42160 in contig AP006618_GR.
KEGG	nfa:nfa42160.
NMPDR	fig|247156.1.peg.4065.
PATRIC	22737976. VBINocFar94200_4215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA42160-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_NOCFA

Accession

Primary (citable) accession number: Q5YRX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program