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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei257ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNFAR247156:G1G24-4207-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:NFA_42160
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196151 – 489Glutamate--tRNA ligaseAdd BLAST489

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa42160

Structurei

3D structure databases

ProteinModelPortaliQ5YRX6
SMRiQ5YRX6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi254 – 258"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
KOiK01885
OMAiHYINTLP
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.1160.10, 1 hit
1.10.8.70, 1 hit
3.40.50.620, 2 hits
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020061 Glu_tRNA_lig_a-bdl
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Q5YRX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEVRVRFCP SPTGTPHVGL IRTALFNWAY ARHTGGTFVF RIEDTDAARD
60 70 80 90 100
SEESYHAILD ALRWLGLTWD EGPEVGGPYG PYRQSQRREL YLDVVRRLLE
110 120 130 140 150
AGEAYESFST PEEVEARHRA AGRDPKLGYD NYDRDLTPEA IEAYRAAGRP
160 170 180 190 200
AVVRLRMPDE DLTWTDLVRG ETTFKAGTVP DFALTRGNGD PLYTLVNPVD
210 220 230 240 250
DASMKITHVL RGEDLLSSTP RQLALYAALR RIGVADFTPE FGHLPFVMGQ
260 270 280 290 300
GNKKLSKRDP ESNLFAHRDR GFIPEGLLNY LALLGWSLAD DRDVFSMSEM
310 320 330 340 350
VAAFDISKVN SNPARFDQKK ADALNAEHIR LLEPGDFAHR LRQFLTEHGH
360 370 380 390 400
IGADVDEKVF AAAAELVQTR IVVLGDAWDL LRFLFAPADE FTVDEAAAAK
410 420 430 440 450
NLGADAVPVL EAAIAALEPL PGWATPVIED ALKTALIDDL GLKPRKAFAP
460 470 480
VRVAVTGSHI SPPLYESLEL LGRELTLARL RSGLEWARQ
Length:489
Mass (Da):54,251
Last modified:November 23, 2004 - v1
Checksum:i1D9403992B960FBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA Translation: BAD59065.1
RefSeqiWP_011210750.1, NC_006361.1

Genome annotation databases

EnsemblBacteriaiBAD59065; BAD59065; NFA_42160
KEGGinfa:NFA_42160

Similar proteinsi

Entry informationi

Entry nameiSYE_NOCFA
AccessioniPrimary (citable) accession number: Q5YRX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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