Q5YRW2 (ILVC_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	NFA_42300

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000226186
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5YRW2 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 25850F8DF5DE2314	FASTA	337	36,103
	10 20 30 40 50 60 MAVEMFYDDD ADLSIIQGRK VAVIGYGSQG HAHSLSLRDS GVEVRVGLAE GSKSRPKAEE 70 80 90 100 110 120 AGLTVGTPAE VSAWADVIML LAPDTAQASI FTNDIEPNLK DGDALFFGHG LNIHFGLIKP 130 140 150 160 170 180 PANVTIGMVA PKGPGHLVRR QFVDGKGVPA LIAIDQDPKG EGQALALSYA KGIGGTRAGV 190 200 210 220 230 240 IKTTFKEETE TDLFGEQAVL CGGTEELVKT GFEVMVEAGY APEMAYFEVL HELKLIVDLM 250 260 270 280 290 300 YEGGIARMNY SVSDTAEFGG YLSGPRVIDA GTKERMKEIL KDIQDGTFVK RLVANVEGGN 310 320 330 KELEGLRKQN AEHPIEVTGA KLRGLMSWVD RPITETA

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD59079.1.
RefSeq	YP_120443.1. NC_006361.1.
3D structure databases
ProteinModelPortal	Q5YRW2.
SMR	Q5YRW2. Positions 3-329.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111184.
GenomeReviews	Gene locus NFA_42300 in contig AP006618_GR.
KEGG	nfa:nfa42300.
NMPDR	fig|247156.1.peg.4078.
PATRIC	22738000. VBINocFar94200_4227.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286525.
OMA	EYANGRG.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA42300-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_NOCFA

Accession

Primary (citable) accession number: Q5YRW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program