Q5YRV8 (ILVD3_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase 3
Short name=DAD 3
EC=4.2.1.9

Gene names

Name:	ilvD3
Ordered Locus Names:	NFA_42340

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase 3 HAMAP MF_00012		PRO_0000225403
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5YRV8 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: C99918EBA3667CA3	FASTA	613	64,330
	10 20 30 40 50 60 MPPLRSRTTT AGRNAAGARA LWRATGLTDS DFGKPIVAIA NSYTQFVPGH VHLKDVGEIV 70 80 90 100 110 120 AEAVRAAGGV PREFHTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMVNA HTADALVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNIPT VFVSGGPMEA GKAVVVGGVA QAPTDLITAI SASANSSVTD 190 200 210 220 230 240 EGLSEVERSA CPTCGSCSGM FTANSMNCLT EALGLALPGN GSTLATHAAR RALFEKAGRV 250 260 270 280 290 300 VVDIANRWYR EDDASVLPRN VANAKAFRNA MALDVAMGGS TNTVLHTLAA AQEGEIDFDL 310 320 330 340 350 360 ETIDEISRKV PCLSKVSPNS DYHMEDVHRA GGIPAILGEL RRAGLLHTDV TTVHTPTLEE 370 380 390 400 410 420 WLDTWDIRSG KASAEAIELF HAAPGGVRTT EPFSTDNRWS SLDTDAAGGC IRDKEHAYTV 430 440 450 460 470 480 EGGLVVLRGN IAPDGAILKT AGIDEDLFSF QGPAVVVESQ EEAVSVILGK KIKPGDVLVV 490 500 510 520 530 540 RYEGPAGGPG MQEMLHPTAF LKGAGLGKQC ALITDGRFSG GTSGLSIGHI SPEAASGGVI 550 560 570 580 590 600 GLVENGDQIR IDVATRTLEV LVDDAVLAER RAKMEASERP WQPVDRDRTV TTALRAYAAL 610 ATSADKGAVR RVP

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD59083.1.
RefSeq	YP_120447.1. NC_006361.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111185.
GenomeReviews	Gene locus NFA_42340 in contig AP006618_GR.
KEGG	nfa:nfa42340.
NMPDR	fig|247156.1.peg.4082.
PATRIC	22738008. VBINocFar94200_4231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA42340-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD3_NOCFA

Accession

Primary (citable) accession number: Q5YRV8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program