Reviewed, UniProtKB/Swiss-Prot Q5YRP8 (COX1A_NOCFA)

Names and origin

Protein names

Recommended name:
    Probable cytochrome c oxidase subunit 1-alpha
    EC=1.9.3.1
Alternative name(s):
    Cytochrome c oxidase polypeptide I-alpha
    Cytochrome aa3 subunit 1-alpha

Gene names

Name:	ctaD1
Ordered Locus Names:	NFA_42940

Organism

Nocardia farcinica [Complete proteome] [HAMAP]

Taxonomic identifier

37329 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B By similarity.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Binds 1 copper B per subunit By similarity. Binds 2 heme groups per subunit By similarity.
Pathway	Energy metabolism; oxidative phosphorylation.
Subunit structure	Associates with subunits II, III and IV to form cytochrome c oxidase By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the heme-copper respiratory oxidase family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Copper Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Probable cytochrome c oxidase subunit 1-alpha		PRO_0000183445
Regions
	Transmembrane	43 – 63	21	Potential
	Transmembrane	90 – 110	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	171 – 191	21	Potential
	Transmembrane	214 – 234	21	Potential
	Transmembrane	259 – 279	21	Potential
	Transmembrane	291 – 311	21	Potential
	Transmembrane	320 – 340	21	Potential
	Transmembrane	360 – 380	21	Potential
	Transmembrane	399 – 419	21	Potential
	Transmembrane	434 – 454	21	Potential
	Transmembrane	477 – 497	21	Potential
Sites
	Metal binding	87	1	Iron (heme A axial ligand) By similarity
	Metal binding	265	1	Copper B By similarity
	Metal binding	269	1	Copper B By similarity
	Metal binding	314	1	Copper B By similarity
	Metal binding	315	1	Copper B By similarity
	Metal binding	398	1	Iron (heme A3 axial ligand) By similarity
	Metal binding	400	1	Iron (heme A axial ligand) By similarity
Amino acid modifications
	Cross-link	265 ↔ 269		1'-histidyl-3'-tyrosine (His-Tyr) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5YRP8-1 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 80C88D7D9C8C2516	FASTA	581	64,744
	10 20 30 40 50 60 MTAVEPRPVP QLEATRPYPA RTGPKGSFLY KMVTTTDPKV LGTMYLVTAM SFFMIGGLMA 70 80 90 100 110 120 LLMRGELARP GLQFLSPEQF NQLFTMHGTI MLLFYATAIV FGFANIILPL QIGAPDVAFP 130 140 150 160 170 180 RLNAFSYWLY AFGATMATAG FITPGGAADF GWTAYVPLTD ILHSPGVGTD LWILGLAVSG 190 200 210 220 230 240 LGTILGGVNM LTTVVCLRAP GMTMFRMPIF TWNIAVTSVL VLLAFPLLTA ALFGLAYDRH 250 260 270 280 290 300 LGGHIYDPAT GGILLYQHLF WFFGHPEVYI IALPFFGIVS EIFPVFSRKP IFGYTTLVYA 310 320 330 340 350 360 TLGIAALSIA VWAHHMYATG AVLLPYFSFM TFLIAVPTGV KFFNWIGTMW RGQLTFESPM 370 380 390 400 410 420 LWSLGFLVTF LFGGLSGVIL ASPPLDFHVT DSYFVVAHFH YVLFGTIVFA TFAGIYFWFP 430 440 450 460 470 480 KITGRMVDER LAKWHFWATF VGFHTTFLVQ HWLGAEGMPR RYADYLPSDG FTTLNTISTI 490 500 510 520 530 540 GAFVLGASML PFIWNIFKSY RYGEVVTVDD PWGYGNSLEW ATTCPPPRHN FYELPRIRSE 550 560 570 580 RPAFELHYPH MIERMRAEAH VGWGSGKHGT PQDDDALLAK K

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD59143.1.
RefSeq	YP_120507.1.
3D structure databases
SMR	Q5YRP8. Positions 37-531.
ModBase	Search...
Genome annotation databases
GeneID	3106270.
GenomeReviews	Gene locus NFA_42940 in contig AP006618_GR.
KEGG	nfa:nfa42940.
NMPDR	fig|247156.1.peg.4137.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG603668.
OMA	GADLWIM.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA42940-MONOMER.
BRENDA	1.9.3.1. 290697.
Family and domain databases
InterPro	IPR000883. Cyt_c_oxidase_su1. IPR014241. Cyt_c_oxidase_su1_bac. [Graphical view]
Gene3D	G3DSA:1.20.210.10. COX1. 1 hit.
PANTHER	PTHR10422. COX1. 1 hit.
Pfam	PF00115. COX1. 1 hit. [Graphical view]
PRINTS	PR01165. CYCOXIDASEI.
TIGRFAMs	TIGR02891. CtaD_CoxA. 1 hit.
PROSITE	PS50855. COX1. 1 hit. PS00077. COX1_CUB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX1A_NOCFA

Accession

Primary (citable) accession number: Q5YRP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	November 23, 2004
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)