Q5YQ94 (ISPH_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Synonyms:	lytB
Ordered Locus Names:	NFA_47950

Organism

Nocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]

Taxonomic identifier

247156 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_0000128847

Sequences

	Sequence		Length	Mass (Da)
	Q5YQ94 [UniParc]. Last modified November 23, 2004. Version 1. Checksum: 9046996C20B43D4D	FASTA	336	36,486
	10 20 30 40 50 60 MSSAIPLNVG IARSAGTATV DADTAKRVLL AEPRGYCAGV DRAVETVEKA LEKHGAPIYV 70 80 90 100 110 120 RKEIVHNRHV VETLRERGVV FVDDTAEVPE GAVVVFSAHG VSPAVHEAAA ARNLHTIDAT 130 140 150 160 170 180 CPLVTKVHQE AKRFARDDYD ILLIGHEGHE EVEGTAGEAP EHVQLVDGPD AVDRVRVRDE 190 200 210 220 230 240 NKVIWLSQTT LSVDETMETV QRLRERFPSL QDPPSDDICY ATQNRQVAVK AMAPECDLVI 250 260 270 280 290 300 VVGSRNSSNS VRLVEVALNA GAKAAHLVDY AREVDPAWLE GVRTIGITSG ASVPEILVRG 310 320 330 VLDMLAEHGY ADVQPVTTAN ETLVFALPRE LRTTRR

References

[1]

"The complete genomic sequence of Nocardia farcinica IFM 10152." Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M. Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed: 15466710] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: IFM 10152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006618 Genomic DNA. Translation: BAD59647.1.
RefSeq	YP_121011.1. NC_006361.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3111229.
GenomeReviews	Gene locus NFA_47950 in contig AP006618_GR.
KEGG	nfa:nfa47950.
NMPDR	fig|247156.1.peg.4609.
PATRIC	22739169. VBINocFar94200_4809.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335228.
OMA	AMAPECD.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	NFAR247156:NFA47950-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_NOCFA

Accession

Primary (citable) accession number: Q5YQ94

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	November 23, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program