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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 2111D-inositol 3-phosphateUniRule annotation
Binding sitei35 – 351UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei90 – 9011D-inositol 3-phosphateUniRule annotation
Binding sitei123 – 12311D-inositol 3-phosphateUniRule annotation
Binding sitei147 – 14711D-inositol 3-phosphateUniRule annotation
Binding sitei167 – 16711D-inositol 3-phosphateUniRule annotation
Binding sitei241 – 2411UDP-GlcNAcUniRule annotation
Binding sitei246 – 2461UDP-GlcNAcUniRule annotation
Binding sitei305 – 3051UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi314 – 3141Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi315 – 3151Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi317 – 3171Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei327 – 3271UDP-GlcNAcUniRule annotation
Binding sitei335 – 3351UDP-GlcNAcUniRule annotation
Metal bindingi341 – 3411MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-5263-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:NFA_51920
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451D-inositol 3-phosphate glycosyltransferasePRO_0000400148Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa51920.

Structurei

3D structure databases

ProteinModelPortaliQ5YP47.
SMRiQ5YP47. Positions 14-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni27 – 282UDP-GlcNAc bindingUniRule annotation
Regioni32 – 3761D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5YP47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGVSQRVDQ RPNRIAVLSV HTSPLAQPGT GDAGGMNVYV LRTAVELAQR
60 70 80 90 100
GIEVEIFTRA TASHIPPVQE AAPGVLVRNV VAGPFEGLDK QDLPTQLCPF
110 120 130 140 150
TAEVLRQEAR QLPGYYDLVH SHYWLSGQVG WLTRDRWRVP LVHTAHTLAA
160 170 180 190 200
VKNAALAEGD APEPVSREIG EKQIIAEADR MVANTAEEAR QLVELYGADR
210 220 230 240 250
DRIDVVPPGA DLTRYRPGDR AAARAELGLA PGEPIVAFVG RIQPLKAPDV
260 270 280 290 300
LVRAAAELLR RDPGRALRVL IVGGPSGSGL QRPDALIELA AELGISERVT
310 320 330 340 350
FLPPQPPERL VQVYRAADLV AVPSYSESFG LVAIEAQASG TPVLAADVGG
360 370 380 390 400
LSTAVRDGAT GLLVRGHETA DWADALGALL GDRDRLRRMG LRAVAHAAGF
410 420 430 440 450
SWAHTAEGLL ESYSAALWEF HGARAGVGAG RMTPNQARSR ALWRRRMGVR

R
Length:451
Mass (Da):48,434
Last modified:November 23, 2004 - v1
Checksum:i501C25C57290984F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD60044.1.
RefSeqiWP_011211726.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD60044; BAD60044; NFA_51920.
KEGGinfa:NFA_51920.
PATRICi22739973. VBINocFar94200_5205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD60044.1.
RefSeqiWP_011211726.1. NC_006361.1.

3D structure databases

ProteinModelPortaliQ5YP47.
SMRiQ5YP47. Positions 14-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi247156.nfa51920.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD60044; BAD60044; NFA_51920.
KEGGinfa:NFA_51920.
PATRICi22739973. VBINocFar94200_5205.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-5263-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_NOCFA
AccessioniPrimary (citable) accession number: Q5YP47
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 23, 2004
Last modified: September 7, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.