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Q5YNB0

- PCKG_NOCFA

UniProt

Q5YNB0 - PCKG_NOCFA

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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Binds 1 manganese ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Binding sitei233 – 2331SubstrateUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi300 – 3001ManganeseUniRule annotation
Binding sitei393 – 3931GTPUniRule annotation
Binding sitei424 – 4241GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi519 – 5224GTPUniRule annotation

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. manganese ion binding Source: UniProtKB-HAMAP
  3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-5551-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:NFA_54790
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
ProteomesiUP000006820: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103612Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa54790.

Structurei

3D structure databases

ProteinModelPortaliQ5YNB0.
SMRiQ5YNB0. Positions 16-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni391 – 3933Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiVIEVYTP.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YNB0 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSATIPGLR GSDGQAPTEH SELLAWVQEV AELTQPERVV WADGSDEEWE
60 70 80 90 100
RLTDQLVEAG TFKRLDPKKK PNSFLALSDP SDVARVESRT FICSRSEADA
110 120 130 140 150
GPTNNWVDPA EMRATMTELY RGCMKGRTMY VVPFCMGPLG AEDPKLGVEI
160 170 180 190 200
TDSEYVVVSM RVMTRMGKAA LEKMGTDRPF VKALHSVGAP LAEGQQDVPW
210 220 230 240 250
PCNDTKYITH FPEDREIWSY GSGYGGNALL GKKCYSLRIA SAMAHDEGWL
260 270 280 290 300
AEHMLILKLI SPENKAYYIA AAFPSACGKT NLAMIQPTVP GWRAETLGDD
310 320 330 340 350
IAWMRFGKDG RLYAVNPEFG FFGVAPGTNH SSNPNAMATM EAGNTVYTNV
360 370 380 390 400
ALTDDNDVWW EGLEGEPDHL IDWKGNDWYL RETETLAAHP NSRYCTPMSQ
410 420 430 440 450
CPILAPEWDD PQGVPISAIL FGGRRKTTVP LVTESFDWQH GVFMGATLSS
460 470 480 490 500
EQTAAAEGKV GTVRRDPMAM LPFLGYHVGD YLDHWIQLGK SADATKLPKI
510 520 530 540 550
FYVNWFRRGE DGRFLWPGFG ENSRVLEWIV GRIEGSADAE ETAIGNVPRA
560 570 580 590 600
AHLDLDGLDV DPADVDEALA VKAEEWRKEI PLIEEWFEFV GDKLPSGVRD
610
EFEALKQRLG
Length:610
Mass (Da):67,710
Last modified:November 23, 2004 - v1
Checksum:iCE462046AC540D88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD60331.1.
RefSeqiYP_121695.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD60331; BAD60331; NFA_54790.
GeneIDi3111363.
KEGGinfa:nfa54790.
PATRICi22740579. VBINocFar94200_5507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006618 Genomic DNA. Translation: BAD60331.1 .
RefSeqi YP_121695.1. NC_006361.1.

3D structure databases

ProteinModelPortali Q5YNB0.
SMRi Q5YNB0. Positions 16-609.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 247156.nfa54790.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD60331 ; BAD60331 ; NFA_54790 .
GeneIDi 3111363.
KEGGi nfa:nfa54790.
PATRICi 22740579. VBINocFar94200_5507.

Phylogenomic databases

eggNOGi COG1274.
KOi K01596.
OMAi VIEVYTP.
OrthoDBi EOG6MPWQS.

Enzyme and pathway databases

UniPathwayi UPA00138 .
BioCyci NFAR247156:GJ9T-5551-MONOMER.

Family and domain databases

Gene3Di 3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPi MF_00452. PEPCK_GTP.
InterProi IPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view ]
PANTHERi PTHR11561. PTHR11561. 1 hit.
Pfami PF00821. PEPCK. 1 hit.
[Graphical view ]
PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMi SSF68923. SSF68923. 1 hit.
PROSITEi PS00505. PEPCK_GTP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.

Entry informationi

Entry nameiPCKG_NOCFA
AccessioniPrimary (citable) accession number: Q5YNB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3