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Q5YNB0

- PCKG_NOCFA

UniProt

Q5YNB0 - PCKG_NOCFA

Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (23 Nov 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

    Cofactori

    Binds 1 manganese ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei85 – 851SubstrateUniRule annotation
    Binding sitei226 – 2261Substrate; via amide nitrogenUniRule annotation
    Metal bindingi233 – 2331ManganeseUniRule annotation
    Binding sitei233 – 2331SubstrateUniRule annotation
    Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
    Binding sitei275 – 2751SubstrateUniRule annotation
    Active sitei277 – 2771UniRule annotation
    Metal bindingi300 – 3001ManganeseUniRule annotation
    Binding sitei393 – 3931GTPUniRule annotation
    Binding sitei424 – 4241GTPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi276 – 2816GTPUniRule annotation
    Nucleotide bindingi519 – 5224GTPUniRule annotation

    GO - Molecular functioni

    1. GTP binding Source: UniProtKB-HAMAP
    2. manganese ion binding Source: UniProtKB-HAMAP
    3. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. gluconeogenesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNFAR247156:GJ9T-5551-MONOMER.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
    Short name:
    PEP carboxykinaseUniRule annotation
    Short name:
    PEPCKUniRule annotation
    Gene namesi
    Name:pckGUniRule annotation
    Ordered Locus Names:NFA_54790
    OrganismiNocardia farcinica (strain IFM 10152)
    Taxonomic identifieri247156 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia
    ProteomesiUP000006820: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103612Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi247156.nfa54790.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5YNB0.
    SMRiQ5YNB0. Positions 16-609.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni391 – 3933Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1274.
    KOiK01596.
    OMAiVIEVYTP.
    OrthoDBiEOG6MPWQS.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5YNB0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSATIPGLR GSDGQAPTEH SELLAWVQEV AELTQPERVV WADGSDEEWE    50
    RLTDQLVEAG TFKRLDPKKK PNSFLALSDP SDVARVESRT FICSRSEADA 100
    GPTNNWVDPA EMRATMTELY RGCMKGRTMY VVPFCMGPLG AEDPKLGVEI 150
    TDSEYVVVSM RVMTRMGKAA LEKMGTDRPF VKALHSVGAP LAEGQQDVPW 200
    PCNDTKYITH FPEDREIWSY GSGYGGNALL GKKCYSLRIA SAMAHDEGWL 250
    AEHMLILKLI SPENKAYYIA AAFPSACGKT NLAMIQPTVP GWRAETLGDD 300
    IAWMRFGKDG RLYAVNPEFG FFGVAPGTNH SSNPNAMATM EAGNTVYTNV 350
    ALTDDNDVWW EGLEGEPDHL IDWKGNDWYL RETETLAAHP NSRYCTPMSQ 400
    CPILAPEWDD PQGVPISAIL FGGRRKTTVP LVTESFDWQH GVFMGATLSS 450
    EQTAAAEGKV GTVRRDPMAM LPFLGYHVGD YLDHWIQLGK SADATKLPKI 500
    FYVNWFRRGE DGRFLWPGFG ENSRVLEWIV GRIEGSADAE ETAIGNVPRA 550
    AHLDLDGLDV DPADVDEALA VKAEEWRKEI PLIEEWFEFV GDKLPSGVRD 600
    EFEALKQRLG 610
    Length:610
    Mass (Da):67,710
    Last modified:November 23, 2004 - v1
    Checksum:iCE462046AC540D88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD60331.1.
    RefSeqiWP_011212013.1. NC_006361.1.
    YP_121695.1. NC_006361.1.

    Genome annotation databases

    EnsemblBacteriaiBAD60331; BAD60331; NFA_54790.
    GeneIDi3111363.
    KEGGinfa:nfa54790.
    PATRICi22740579. VBINocFar94200_5507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP006618 Genomic DNA. Translation: BAD60331.1 .
    RefSeqi WP_011212013.1. NC_006361.1.
    YP_121695.1. NC_006361.1.

    3D structure databases

    ProteinModelPortali Q5YNB0.
    SMRi Q5YNB0. Positions 16-609.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 247156.nfa54790.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD60331 ; BAD60331 ; NFA_54790 .
    GeneIDi 3111363.
    KEGGi nfa:nfa54790.
    PATRICi 22740579. VBINocFar94200_5507.

    Phylogenomic databases

    eggNOGi COG1274.
    KOi K01596.
    OMAi VIEVYTP.
    OrthoDBi EOG6MPWQS.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    BioCyci NFAR247156:GJ9T-5551-MONOMER.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: IFM 10152.

    Entry informationi

    Entry nameiPCKG_NOCFA
    AccessioniPrimary (citable) accession number: Q5YNB0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 25, 2005
    Last sequence update: November 23, 2004
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3