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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathway:igluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Metal bindingi233 – 2331ManganeseUniRule annotation
Metal bindingi253 – 2531Manganese; via tele nitrogenUniRule annotation
Binding sitei275 – 2751SubstrateUniRule annotation
Active sitei277 – 2771UniRule annotation
Metal bindingi300 – 3001ManganeseUniRule annotation
Binding sitei393 – 3931GTPUniRule annotation
Binding sitei424 – 4241GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi276 – 2816GTPUniRule annotation
Nucleotide bindingi519 – 5224GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNFAR247156:GJ9T-5551-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:NFA_54790
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
ProteomesiUP000006820 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Phosphoenolpyruvate carboxykinase [GTP]PRO_0000103612Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa54790.

Structurei

3D structure databases

ProteinModelPortaliQ5YNB0.
SMRiQ5YNB0. Positions 16-609.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni224 – 2263Substrate bindingUniRule annotation
Regioni391 – 3933Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5YNB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATIPGLR GSDGQAPTEH SELLAWVQEV AELTQPERVV WADGSDEEWE
60 70 80 90 100
RLTDQLVEAG TFKRLDPKKK PNSFLALSDP SDVARVESRT FICSRSEADA
110 120 130 140 150
GPTNNWVDPA EMRATMTELY RGCMKGRTMY VVPFCMGPLG AEDPKLGVEI
160 170 180 190 200
TDSEYVVVSM RVMTRMGKAA LEKMGTDRPF VKALHSVGAP LAEGQQDVPW
210 220 230 240 250
PCNDTKYITH FPEDREIWSY GSGYGGNALL GKKCYSLRIA SAMAHDEGWL
260 270 280 290 300
AEHMLILKLI SPENKAYYIA AAFPSACGKT NLAMIQPTVP GWRAETLGDD
310 320 330 340 350
IAWMRFGKDG RLYAVNPEFG FFGVAPGTNH SSNPNAMATM EAGNTVYTNV
360 370 380 390 400
ALTDDNDVWW EGLEGEPDHL IDWKGNDWYL RETETLAAHP NSRYCTPMSQ
410 420 430 440 450
CPILAPEWDD PQGVPISAIL FGGRRKTTVP LVTESFDWQH GVFMGATLSS
460 470 480 490 500
EQTAAAEGKV GTVRRDPMAM LPFLGYHVGD YLDHWIQLGK SADATKLPKI
510 520 530 540 550
FYVNWFRRGE DGRFLWPGFG ENSRVLEWIV GRIEGSADAE ETAIGNVPRA
560 570 580 590 600
AHLDLDGLDV DPADVDEALA VKAEEWRKEI PLIEEWFEFV GDKLPSGVRD
610
EFEALKQRLG
Length:610
Mass (Da):67,710
Last modified:November 23, 2004 - v1
Checksum:iCE462046AC540D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD60331.1.
RefSeqiWP_011212013.1. NC_006361.1.

Genome annotation databases

EnsemblBacteriaiBAD60331; BAD60331; NFA_54790.
KEGGinfa:nfa54790.
PATRICi22740579. VBINocFar94200_5507.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA. Translation: BAD60331.1.
RefSeqiWP_011212013.1. NC_006361.1.

3D structure databases

ProteinModelPortaliQ5YNB0.
SMRiQ5YNB0. Positions 16-609.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi247156.nfa54790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD60331; BAD60331; NFA_54790.
KEGGinfa:nfa54790.
PATRICi22740579. VBINocFar94200_5507.

Phylogenomic databases

eggNOGiCOG1274.
KOiK01596.
OMAiWMRFGED.
OrthoDBiEOG6MPWQS.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciNFAR247156:GJ9T-5551-MONOMER.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: IFM 10152.

Entry informationi

Entry nameiPCKG_NOCFA
AccessioniPrimary (citable) accession number: Q5YNB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 23, 2004
Last modified: July 22, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.