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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Nocardia farcinica (strain IFM 10152)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85SubstrateUniRule annotation1
Metal bindingi233ManganeseUniRule annotation1
Metal bindingi253Manganese; via tele nitrogenUniRule annotation1
Binding sitei275SubstrateUniRule annotation1
Active sitei277UniRule annotation1
Metal bindingi300ManganeseUniRule annotation1
Binding sitei393GTPUniRule annotation1
Binding sitei424GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi276 – 281GTPUniRule annotation6
Nucleotide bindingi519 – 522GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:NFA_54790
OrganismiNocardia farcinica (strain IFM 10152)
Taxonomic identifieri247156 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesNocardiaceaeNocardia
Proteomesi
  • UP000006820 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036121 – 610Phosphoenolpyruvate carboxykinase [GTP]Add BLAST610

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi247156.nfa54790

Structurei

3D structure databases

ProteinModelPortaliQ5YNB0
SMRiQ5YNB0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni224 – 226Substrate bindingUniRule annotation3
Regioni391 – 393Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M Bacteria
COG1274 LUCA
KOiK01596
OMAiCWLALTD
OrthoDBiPOG091H0B4F

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5YNB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSATIPGLR GSDGQAPTEH SELLAWVQEV AELTQPERVV WADGSDEEWE
60 70 80 90 100
RLTDQLVEAG TFKRLDPKKK PNSFLALSDP SDVARVESRT FICSRSEADA
110 120 130 140 150
GPTNNWVDPA EMRATMTELY RGCMKGRTMY VVPFCMGPLG AEDPKLGVEI
160 170 180 190 200
TDSEYVVVSM RVMTRMGKAA LEKMGTDRPF VKALHSVGAP LAEGQQDVPW
210 220 230 240 250
PCNDTKYITH FPEDREIWSY GSGYGGNALL GKKCYSLRIA SAMAHDEGWL
260 270 280 290 300
AEHMLILKLI SPENKAYYIA AAFPSACGKT NLAMIQPTVP GWRAETLGDD
310 320 330 340 350
IAWMRFGKDG RLYAVNPEFG FFGVAPGTNH SSNPNAMATM EAGNTVYTNV
360 370 380 390 400
ALTDDNDVWW EGLEGEPDHL IDWKGNDWYL RETETLAAHP NSRYCTPMSQ
410 420 430 440 450
CPILAPEWDD PQGVPISAIL FGGRRKTTVP LVTESFDWQH GVFMGATLSS
460 470 480 490 500
EQTAAAEGKV GTVRRDPMAM LPFLGYHVGD YLDHWIQLGK SADATKLPKI
510 520 530 540 550
FYVNWFRRGE DGRFLWPGFG ENSRVLEWIV GRIEGSADAE ETAIGNVPRA
560 570 580 590 600
AHLDLDGLDV DPADVDEALA VKAEEWRKEI PLIEEWFEFV GDKLPSGVRD
610
EFEALKQRLG
Length:610
Mass (Da):67,710
Last modified:November 23, 2004 - v1
Checksum:iCE462046AC540D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006618 Genomic DNA Translation: BAD60331.1
RefSeqiWP_011212013.1, NC_006361.1

Genome annotation databases

EnsemblBacteriaiBAD60331; BAD60331; NFA_54790
KEGGinfa:NFA_54790

Similar proteinsi

Entry informationi

Entry nameiPCKG_NOCFA
AccessioniPrimary (citable) accession number: Q5YNB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: November 23, 2004
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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