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Q5YNA5 (TRMB_NOCFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:NFA_54840
OrganismNocardia farcinica (strain IFM 10152) [Complete proteome] [HAMAP]
Taxonomic identifier247156 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeNocardiaceaeNocardia

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171365

Regions

Region247 – 2504Substrate binding By similarity

Sites

Active site1661 By similarity
Binding site911S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1661S-adenosyl-L-methionine By similarity
Binding site1701Substrate By similarity
Binding site2021Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5YNA5 [UniParc].

Last modified November 23, 2004. Version 1.
Checksum: 047088073EC83495

FASTA26928,933
        10         20         30         40         50         60 
MDGVNDAANH TVESVPGRPS TASAPLEAGR RSPTGSRLYP RVTSFRSRRG ALTPNQQGAW 

        70         80         90        100        110        120 
DRMWPRIGRE VGDEPLDADA WFGRQAPLVI EIGCGTGTAT AAMAAAEPHV NLIGIEVYQP 

       130        140        150        160        170        180 
GLAQLVQRIE RDGIDNIRLL RGDAVDVLEH MIAAESLTGV RVFFPDPWPK ARHHKRRLLQ 

       190        200        210        220        230        240 
PATVALIAGR LRPGGVLHVA TDHAGYAEHI AAVGAAEPLL VGLNEATGGD DERARTLAPI 

       250        260 
GLDRPVTKFE DKAHRAGSAI TEFVWGKIE 

« Hide

References

[1]"The complete genomic sequence of Nocardia farcinica IFM 10152."
Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K., Shiba T., Hattori M.
Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: IFM 10152.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006618 Genomic DNA. Translation: BAD60336.1.
RefSeqYP_121700.1. NC_006361.1.

3D structure databases

ProteinModelPortalQ5YNA5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING247156.nfa54840.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD60336; BAD60336; NFA_54840.
GeneID3110508.
KEGGnfa:nfa54840.
PATRIC22740593. VBINocFar94200_5514.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMALDKDHTI.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycNFAR247156:GJ9T-5556-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_NOCFA
AccessionPrimary (citable) accession number: Q5YNA5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: November 23, 2004
Last modified: April 16, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways